Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology

Abstract Skeletal muscle physiology and the mechanisms of muscle diseases can be effectively studied by an in-vitro tissue model produced by muscle tissue engineering. Engineered human cell-based tissues are required more than ever because of the advantages they bring as tissue models in research st...

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Autores principales:	Hironobu Takahashi, Tatsuya Shimizu, Teruo Okano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Myofibers Contractile Ability Native Muscle Tissue Tissue Model Pulse Electrical Stimulation (EPS) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02047d618fc8401c9c4c8a69fac90a4c
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spelling	oai:doaj.org-article:02047d618fc8401c9c4c8a69fac90a4c2021-12-02T15:08:13ZEngineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology10.1038/s41598-018-32163-12045-2322https://doaj.org/article/02047d618fc8401c9c4c8a69fac90a4c2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32163-1https://doaj.org/toc/2045-2322Abstract Skeletal muscle physiology and the mechanisms of muscle diseases can be effectively studied by an in-vitro tissue model produced by muscle tissue engineering. Engineered human cell-based tissues are required more than ever because of the advantages they bring as tissue models in research studies. This study reports on a production method of a human skeletal myofiber sheet that demonstrates biomimetic properties including the aligned structure of myofibers, basement membrane-like structure of the extracellular matrix, and unidirectional contractile ability. The contractile ability and drug responsibility shown in this study indicate that this engineered muscle tissue has potential as a human cell-based tissue model for clinically relevant in-vitro studies in muscle physiology and drug discovery. Moreover, this engineered tissue can be used to better understand the relationships between mechanical stress and myogenesis, including muscle growth and regeneration. In this study, periodic exercise induced by continuous electrical pulse stimulation enhanced the contractile ability of the engineered myofibers and the secretion of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) from the exercising myofibers. Since the physiology of skeletal muscle is directly related to mechanical stress, these features point to application as a tissue model and platform for future biological studies of skeletal muscle including muscle metabolism, muscle atrophy and muscle regeneration.Hironobu TakahashiTatsuya ShimizuTeruo OkanoNature PortfolioarticleMyofibersContractile AbilityNative Muscle TissueTissue ModelPulse Electrical Stimulation (EPS)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Myofibers Contractile Ability Native Muscle Tissue Tissue Model Pulse Electrical Stimulation (EPS) Medicine R Science Q
spellingShingle	Myofibers Contractile Ability Native Muscle Tissue Tissue Model Pulse Electrical Stimulation (EPS) Medicine R Science Q Hironobu Takahashi Tatsuya Shimizu Teruo Okano Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
description	Abstract Skeletal muscle physiology and the mechanisms of muscle diseases can be effectively studied by an in-vitro tissue model produced by muscle tissue engineering. Engineered human cell-based tissues are required more than ever because of the advantages they bring as tissue models in research studies. This study reports on a production method of a human skeletal myofiber sheet that demonstrates biomimetic properties including the aligned structure of myofibers, basement membrane-like structure of the extracellular matrix, and unidirectional contractile ability. The contractile ability and drug responsibility shown in this study indicate that this engineered muscle tissue has potential as a human cell-based tissue model for clinically relevant in-vitro studies in muscle physiology and drug discovery. Moreover, this engineered tissue can be used to better understand the relationships between mechanical stress and myogenesis, including muscle growth and regeneration. In this study, periodic exercise induced by continuous electrical pulse stimulation enhanced the contractile ability of the engineered myofibers and the secretion of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) from the exercising myofibers. Since the physiology of skeletal muscle is directly related to mechanical stress, these features point to application as a tissue model and platform for future biological studies of skeletal muscle including muscle metabolism, muscle atrophy and muscle regeneration.
format	article
author	Hironobu Takahashi Tatsuya Shimizu Teruo Okano
author_facet	Hironobu Takahashi Tatsuya Shimizu Teruo Okano
author_sort	Hironobu Takahashi
title	Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
title_short	Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
title_full	Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
title_fullStr	Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
title_full_unstemmed	Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology
title_sort	engineered human contractile myofiber sheets as a platform for studies of skeletal muscle physiology
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/02047d618fc8401c9c4c8a69fac90a4c
work_keys_str_mv	AT hironobutakahashi engineeredhumancontractilemyofibersheetsasaplatformforstudiesofskeletalmusclephysiology AT tatsuyashimizu engineeredhumancontractilemyofibersheetsasaplatformforstudiesofskeletalmusclephysiology AT teruookano engineeredhumancontractilemyofibersheetsasaplatformforstudiesofskeletalmusclephysiology
_version_	1718388231681081344

Engineered Human Contractile Myofiber Sheets as a Platform for Studies of Skeletal Muscle Physiology

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