Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae

Abstract Objectives In streptococci, the type M resistance to macrolides is due to the mef(A)–msr(D) efflux transport system of the ATP-Binding cassette (ABC) superfamily, where it is proposed that mef(A) codes for the transmembrane channel and msr(D) for the two ATP-binding domains. Phage ϕ1207.3 o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valeria Fox, Francesco Santoro, Gianni Pozzi, Francesco Iannelli
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Mef(A) Msr(D) Macrolide efflux Streptococcus pyogenes Streptococcus pneumoniae ABC-transporter Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/0204ba6d66fb4f25a21adf1da90dc25e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0204ba6d66fb4f25a21adf1da90dc25e
record_format	dspace
spelling	oai:doaj.org-article:0204ba6d66fb4f25a21adf1da90dc25e2021-11-28T12:25:18ZPredicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae10.1186/s13104-021-05856-61756-0500https://doaj.org/article/0204ba6d66fb4f25a21adf1da90dc25e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13104-021-05856-6https://doaj.org/toc/1756-0500Abstract Objectives In streptococci, the type M resistance to macrolides is due to the mef(A)–msr(D) efflux transport system of the ATP-Binding cassette (ABC) superfamily, where it is proposed that mef(A) codes for the transmembrane channel and msr(D) for the two ATP-binding domains. Phage ϕ1207.3 of Streptococcus pyogenes, carrying the mef(A)–msr(D) gene pair, is able to transfer the macrolide efflux phenotype to Streptococcus pneumoniae. Deletion of mef(A) in pneumococcal ϕ1207.3-carrying strains did not affect erythromycin efflux. In order to identify candidate genes likely involved in complementation of mef(A) deletion, the Mef(A) amino acid sequence was used as probe for database searching. Results In silico analysis identified 3 putative candidates in the S. pneumoniae R6 genome, namely spr0971, spr1023 and spr1932. Isogenic deletion mutants of each candidate gene were constructed and used in erythromycin sensitivity assays to investigate their contribution to mef(A) complementation. Since no change in erythromycin sensitivity was observed compared to the parental strain, we produced double and triple mutants to assess the potential synergic activity of the selected genes. Also these mutants did not complement the mef(A) function.Valeria FoxFrancesco SantoroGianni PozziFrancesco IannelliBMCarticleMef(A)Msr(D)Macrolide effluxStreptococcus pyogenesStreptococcus pneumoniaeABC-transporterMedicineRBiology (General)QH301-705.5Science (General)Q1-390ENBMC Research Notes, Vol 14, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mef(A) Msr(D) Macrolide efflux Streptococcus pyogenes Streptococcus pneumoniae ABC-transporter Medicine R Biology (General) QH301-705.5 Science (General) Q1-390
spellingShingle	Mef(A) Msr(D) Macrolide efflux Streptococcus pyogenes Streptococcus pneumoniae ABC-transporter Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Valeria Fox Francesco Santoro Gianni Pozzi Francesco Iannelli Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
description	Abstract Objectives In streptococci, the type M resistance to macrolides is due to the mef(A)–msr(D) efflux transport system of the ATP-Binding cassette (ABC) superfamily, where it is proposed that mef(A) codes for the transmembrane channel and msr(D) for the two ATP-binding domains. Phage ϕ1207.3 of Streptococcus pyogenes, carrying the mef(A)–msr(D) gene pair, is able to transfer the macrolide efflux phenotype to Streptococcus pneumoniae. Deletion of mef(A) in pneumococcal ϕ1207.3-carrying strains did not affect erythromycin efflux. In order to identify candidate genes likely involved in complementation of mef(A) deletion, the Mef(A) amino acid sequence was used as probe for database searching. Results In silico analysis identified 3 putative candidates in the S. pneumoniae R6 genome, namely spr0971, spr1023 and spr1932. Isogenic deletion mutants of each candidate gene were constructed and used in erythromycin sensitivity assays to investigate their contribution to mef(A) complementation. Since no change in erythromycin sensitivity was observed compared to the parental strain, we produced double and triple mutants to assess the potential synergic activity of the selected genes. Also these mutants did not complement the mef(A) function.
format	article
author	Valeria Fox Francesco Santoro Gianni Pozzi Francesco Iannelli
author_facet	Valeria Fox Francesco Santoro Gianni Pozzi Francesco Iannelli
author_sort	Valeria Fox
title	Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
title_short	Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
title_full	Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
title_fullStr	Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
title_full_unstemmed	Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae
title_sort	predicted transmembrane proteins with homology to mef(a) are not responsible for complementing mef(a) deletion in the mef(a)–msr(d) macrolide efflux system in streptococcus pneumoniae
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0204ba6d66fb4f25a21adf1da90dc25e
work_keys_str_mv	AT valeriafox predictedtransmembraneproteinswithhomologytomefaarenotresponsibleforcomplementingmefadeletioninthemefamsrdmacrolideeffluxsysteminstreptococcuspneumoniae AT francescosantoro predictedtransmembraneproteinswithhomologytomefaarenotresponsibleforcomplementingmefadeletioninthemefamsrdmacrolideeffluxsysteminstreptococcuspneumoniae AT giannipozzi predictedtransmembraneproteinswithhomologytomefaarenotresponsibleforcomplementingmefadeletioninthemefamsrdmacrolideeffluxsysteminstreptococcuspneumoniae AT francescoiannelli predictedtransmembraneproteinswithhomologytomefaarenotresponsibleforcomplementingmefadeletioninthemefamsrdmacrolideeffluxsysteminstreptococcuspneumoniae
_version_	1718407933111304192

Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)–msr(D) macrolide efflux system in Streptococcus pneumoniae

Ejemplares similares