Early cell death detection with digital holographic microscopy.

<h4>Background</h4>Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicolas Pavillon, Jonas Kühn, Corinne Moratal, Pascal Jourdain, Christian Depeursinge, Pierre J Magistretti, Pierre Marquet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/020735896bc74ea8970c7ce66e2759cc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:020735896bc74ea8970c7ce66e2759cc
record_format	dspace
spelling	oai:doaj.org-article:020735896bc74ea8970c7ce66e2759cc2021-11-18T07:29:06ZEarly cell death detection with digital holographic microscopy.1932-620310.1371/journal.pone.0030912https://doaj.org/article/020735896bc74ea8970c7ce66e2759cc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22303471/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM) dedicated to early and label-free detection of cell death.<h4>Methods and findings</h4>We provide quantitative measurements of phase signal obtained on mouse cortical neurons, and caused by early neuronal cell volume regulation triggered by excitotoxic concentrations of L-glutamate. We show that the efficiency of this early regulation of cell volume detected by DHM, is correlated with the occurrence of subsequent neuronal death assessed with the widely accepted trypan blue method for detection of cell viability.<h4>Conclusions</h4>The determination of the phase signal by DHM provides a simple and rapid optical method for the early detection of cell death.Nicolas PavillonJonas KühnCorinne MoratalPascal JourdainChristian DepeursingePierre J MagistrettiPierre MarquetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30912 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolas Pavillon Jonas Kühn Corinne Moratal Pascal Jourdain Christian Depeursinge Pierre J Magistretti Pierre Marquet Early cell death detection with digital holographic microscopy.
description	<h4>Background</h4>Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM) dedicated to early and label-free detection of cell death.<h4>Methods and findings</h4>We provide quantitative measurements of phase signal obtained on mouse cortical neurons, and caused by early neuronal cell volume regulation triggered by excitotoxic concentrations of L-glutamate. We show that the efficiency of this early regulation of cell volume detected by DHM, is correlated with the occurrence of subsequent neuronal death assessed with the widely accepted trypan blue method for detection of cell viability.<h4>Conclusions</h4>The determination of the phase signal by DHM provides a simple and rapid optical method for the early detection of cell death.
format	article
author	Nicolas Pavillon Jonas Kühn Corinne Moratal Pascal Jourdain Christian Depeursinge Pierre J Magistretti Pierre Marquet
author_facet	Nicolas Pavillon Jonas Kühn Corinne Moratal Pascal Jourdain Christian Depeursinge Pierre J Magistretti Pierre Marquet
author_sort	Nicolas Pavillon
title	Early cell death detection with digital holographic microscopy.
title_short	Early cell death detection with digital holographic microscopy.
title_full	Early cell death detection with digital holographic microscopy.
title_fullStr	Early cell death detection with digital holographic microscopy.
title_full_unstemmed	Early cell death detection with digital holographic microscopy.
title_sort	early cell death detection with digital holographic microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/020735896bc74ea8970c7ce66e2759cc
work_keys_str_mv	AT nicolaspavillon earlycelldeathdetectionwithdigitalholographicmicroscopy AT jonaskuhn earlycelldeathdetectionwithdigitalholographicmicroscopy AT corinnemoratal earlycelldeathdetectionwithdigitalholographicmicroscopy AT pascaljourdain earlycelldeathdetectionwithdigitalholographicmicroscopy AT christiandepeursinge earlycelldeathdetectionwithdigitalholographicmicroscopy AT pierrejmagistretti earlycelldeathdetectionwithdigitalholographicmicroscopy AT pierremarquet earlycelldeathdetectionwithdigitalholographicmicroscopy
_version_	1718423389070163968

Early cell death detection with digital holographic microscopy.

Ejemplares similares