Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>

Oleaginous yeast <i>Rhodosporidium toruloides</i> has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon and nitrogen ratios with concurrent lipid agglomeration remains elusive. Here, metabolomics adaptations of the &l...

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Autores principales:	Farha Deeba, Kukkala Kiran Kumar, Girish H. Rajacharya, Naseem A. Gaur
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Rhodosporidium toruloides</i> triacylglycerol metabolomics biofuel Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0207be0942e34e039add3aade611f8ed
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spelling	oai:doaj.org-article:0207be0942e34e039add3aade611f8ed2021-11-25T18:06:15ZMetabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>10.3390/jof71109672309-608Xhttps://doaj.org/article/0207be0942e34e039add3aade611f8ed2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/967https://doaj.org/toc/2309-608XOleaginous yeast <i>Rhodosporidium toruloides</i> has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon and nitrogen ratios with concurrent lipid agglomeration remains elusive. Here, metabolomics adaptations of the <i>R. toruloides</i> in response to varying glucose and nitrogen concentrations have been investigated. In preliminary screening we found that 5% glucose (<i>w</i>/<i>v</i>) was optimal for further analysis in <i>Rhodosporidium toruloides</i> 3641. Hereafter, the effect of complementation to increase lipid agglomeration was evaluated with different nitrogen sources and their concentration. The results obtained illustrated that the biomass (13 g/L) and lipid (9.1 g/L) production were maximum on 5% (<i>w</i>/<i>v</i>) glucose and 0.12% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>. Furthermore, to shed lights on lipid accumulation induced by nitrogen-limitation, we performed metabolomic analysis of the oleaginous yeast <i>R. toruloides</i> 3641. Significant changes were observed in metabolite concentrations by qualitative metabolomics through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), which were mapped onto the governing metabolic pathways. Notable finding in this strain concerns glycerol and CDP-DAG metabolism wherein reduced production of glycerol and phospholipids induced a bypass leading to enhanced de-novo triacylglyceride synthesis. Collectively, our findings help in understanding the central carbon metabolism of <i>R. toruloides</i> which may assist in developing rationale metabolic models and engineering efforts in this organism.Farha DeebaKukkala Kiran KumarGirish H. RajacharyaNaseem A. GaurMDPI AGarticle<i>Rhodosporidium toruloides</i>triacylglycerolmetabolomicsbiofuelBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 967, p 967 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Rhodosporidium toruloides</i> triacylglycerol metabolomics biofuel Biology (General) QH301-705.5
spellingShingle	<i>Rhodosporidium toruloides</i> triacylglycerol metabolomics biofuel Biology (General) QH301-705.5 Farha Deeba Kukkala Kiran Kumar Girish H. Rajacharya Naseem A. Gaur Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>
description	Oleaginous yeast <i>Rhodosporidium toruloides</i> has great biotechnological potential and scientific interest, yet the molecular rationale of its cellular behavior to carbon and nitrogen ratios with concurrent lipid agglomeration remains elusive. Here, metabolomics adaptations of the <i>R. toruloides</i> in response to varying glucose and nitrogen concentrations have been investigated. In preliminary screening we found that 5% glucose (<i>w</i>/<i>v</i>) was optimal for further analysis in <i>Rhodosporidium toruloides</i> 3641. Hereafter, the effect of complementation to increase lipid agglomeration was evaluated with different nitrogen sources and their concentration. The results obtained illustrated that the biomass (13 g/L) and lipid (9.1 g/L) production were maximum on 5% (<i>w</i>/<i>v</i>) glucose and 0.12% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>. Furthermore, to shed lights on lipid accumulation induced by nitrogen-limitation, we performed metabolomic analysis of the oleaginous yeast <i>R. toruloides</i> 3641. Significant changes were observed in metabolite concentrations by qualitative metabolomics through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), which were mapped onto the governing metabolic pathways. Notable finding in this strain concerns glycerol and CDP-DAG metabolism wherein reduced production of glycerol and phospholipids induced a bypass leading to enhanced de-novo triacylglyceride synthesis. Collectively, our findings help in understanding the central carbon metabolism of <i>R. toruloides</i> which may assist in developing rationale metabolic models and engineering efforts in this organism.
format	article
author	Farha Deeba Kukkala Kiran Kumar Girish H. Rajacharya Naseem A. Gaur
author_facet	Farha Deeba Kukkala Kiran Kumar Girish H. Rajacharya Naseem A. Gaur
author_sort	Farha Deeba
title	Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>
title_short	Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>
title_full	Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>
title_fullStr	Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>
title_full_unstemmed	Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>
title_sort	metabolomic profiling revealed diversion of cytidinediphosphate-diacylglycerol and glycerol pathway towards denovo triacylglycerol synthesis in <i>rhodosporidium toruloides</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0207be0942e34e039add3aade611f8ed
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_version_	1718411599811706880

Metabolomic Profiling Revealed Diversion of Cytidinediphosphate-Diacylglycerol and Glycerol Pathway towards Denovo Triacylglycerol Synthesis in <i>Rhodosporidium toruloides</i>

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