MiR-16-5p suppresses breast cancer proliferation by targeting ANLN

Abstract Background In recent years, gene expression-based analysis has been used for disease biomarker discovery, providing ways for better diagnosis, leading to improvement of clinical treatment efficacy. This study aimed to explore the role of miR-16-5p and ANLN in breast cancer (BC). Methods Coh...

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Autores principales:	Ziming Wang, Siyuan Hu, Xinyang Li, Zhiwei Liu, Danyang Han, Yukun Wang, Limin Wei, Guangping Zhang, Xinshuai Wang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Breast cancer Bioinformatics ANLN miR-16-5p Proliferation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/0208350eaf2f41979c3f171f9a6928a8
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spelling	oai:doaj.org-article:0208350eaf2f41979c3f171f9a6928a82021-11-14T12:29:57ZMiR-16-5p suppresses breast cancer proliferation by targeting ANLN10.1186/s12885-021-08914-11471-2407https://doaj.org/article/0208350eaf2f41979c3f171f9a6928a82021-11-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-08914-1https://doaj.org/toc/1471-2407Abstract Background In recent years, gene expression-based analysis has been used for disease biomarker discovery, providing ways for better diagnosis, leading to improvement of clinical treatment efficacy. This study aimed to explore the role of miR-16-5p and ANLN in breast cancer (BC). Methods Cohort datasets of BC were obtained from the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) and analyzed by bioinformatics tools. qRT-PCR and western blotting were applied to validate ANLN and its protein expression. A dual-luciferase reporter assay was used to prove the regulatory relationship of miR-16-5p and ANLN. Finally, MTT, wound healing, Transwell invasion and flow cytometry analyses of the cell cycle and apoptosis were performed to assess cell proliferation, migration, invasion, cell cycle and apoptosis, respectively. Results A total of 195 differentially expressed genes (DEGs) and 50 overlapping microRNAs (miRNAs) were identified. Among these DEGs and miRNAs, ANLN, associated with poor overall survival in BC, overlapped in the GSE29431, GSE42568, TCGA and GEPIA2 databases. Moreover, ANLN was highly expressed, while miR-16-5p was lower in BC cells than in breast epithelial cells. Then, we confirmed that ANLN was directly targeted by miR-16-5p in BC cells. Over-expression of miR-16-5p and knock-down of ANLN remarkably inhibited cell proliferation and migration as well as cell invasion, arrested the cells in G2/M phase and induced apoptosis in BC cells. Conclusions These findings suggest that miR-16-5p restrains proliferation, migration and invasion while affecting cell cycle and promotes apoptosis by regulating ANLN, thereby providing novel candidate biomarkers for the diagnosis and treatment of BC.Ziming WangSiyuan HuXinyang LiZhiwei LiuDanyang HanYukun WangLimin WeiGuangping ZhangXinshuai WangBMCarticleBreast cancerBioinformaticsANLNmiR-16-5pProliferationNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Breast cancer Bioinformatics ANLN miR-16-5p Proliferation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Breast cancer Bioinformatics ANLN miR-16-5p Proliferation Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Ziming Wang Siyuan Hu Xinyang Li Zhiwei Liu Danyang Han Yukun Wang Limin Wei Guangping Zhang Xinshuai Wang MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
description	Abstract Background In recent years, gene expression-based analysis has been used for disease biomarker discovery, providing ways for better diagnosis, leading to improvement of clinical treatment efficacy. This study aimed to explore the role of miR-16-5p and ANLN in breast cancer (BC). Methods Cohort datasets of BC were obtained from the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) and analyzed by bioinformatics tools. qRT-PCR and western blotting were applied to validate ANLN and its protein expression. A dual-luciferase reporter assay was used to prove the regulatory relationship of miR-16-5p and ANLN. Finally, MTT, wound healing, Transwell invasion and flow cytometry analyses of the cell cycle and apoptosis were performed to assess cell proliferation, migration, invasion, cell cycle and apoptosis, respectively. Results A total of 195 differentially expressed genes (DEGs) and 50 overlapping microRNAs (miRNAs) were identified. Among these DEGs and miRNAs, ANLN, associated with poor overall survival in BC, overlapped in the GSE29431, GSE42568, TCGA and GEPIA2 databases. Moreover, ANLN was highly expressed, while miR-16-5p was lower in BC cells than in breast epithelial cells. Then, we confirmed that ANLN was directly targeted by miR-16-5p in BC cells. Over-expression of miR-16-5p and knock-down of ANLN remarkably inhibited cell proliferation and migration as well as cell invasion, arrested the cells in G2/M phase and induced apoptosis in BC cells. Conclusions These findings suggest that miR-16-5p restrains proliferation, migration and invasion while affecting cell cycle and promotes apoptosis by regulating ANLN, thereby providing novel candidate biomarkers for the diagnosis and treatment of BC.
format	article
author	Ziming Wang Siyuan Hu Xinyang Li Zhiwei Liu Danyang Han Yukun Wang Limin Wei Guangping Zhang Xinshuai Wang
author_facet	Ziming Wang Siyuan Hu Xinyang Li Zhiwei Liu Danyang Han Yukun Wang Limin Wei Guangping Zhang Xinshuai Wang
author_sort	Ziming Wang
title	MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
title_short	MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
title_full	MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
title_fullStr	MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
title_full_unstemmed	MiR-16-5p suppresses breast cancer proliferation by targeting ANLN
title_sort	mir-16-5p suppresses breast cancer proliferation by targeting anln
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0208350eaf2f41979c3f171f9a6928a8
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MiR-16-5p suppresses breast cancer proliferation by targeting ANLN

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