Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming

Among the diverse array of heat shock proteins across the three domains of life, mitochondria-targeted small heat shock proteins (sHSPs) are evolved in the plant lineage. However, they remained mysterious and understudied. In this study, we reported a systematic study of a novel mitochondria-targete...

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Autores principales:	Muslima Khatun, Bhabesh Borphukan, Iftekhar Alam, Chaman Ara Keya, Varakumar Panditi, Haseena Khan, Saaimatul Huq, Malireddy K. Reddy, Md. Salimullah
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	eggplant mitochondria chaperon abiotic stress heat tolerance small heat shock protein Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0209d1218a1c4bf1bbf6f487497e70bc
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spelling	oai:doaj.org-article:0209d1218a1c4bf1bbf6f487497e70bc2021-11-30T12:12:09ZMitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming1664-462X10.3389/fpls.2021.741898https://doaj.org/article/0209d1218a1c4bf1bbf6f487497e70bc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.741898/fullhttps://doaj.org/toc/1664-462XAmong the diverse array of heat shock proteins across the three domains of life, mitochondria-targeted small heat shock proteins (sHSPs) are evolved in the plant lineage. However, they remained mysterious and understudied. In this study, we reported a systematic study of a novel mitochondria-targeted nuclear sHSP from eggplant (Solanum melongena L.; SmsHSP24.1). Differential expression of SmsHSP24.1 indicated its positive role exerted during stress conditions. Escherichia coli-BL21 cell line overexpressing the SmsHSP24.1 showed excellent thermo-tolerance ability, tolerating up to 52°C. Spectrometry and electron microscopy revealed a multimeric structure of the protein which acted as a molecular chaperone at high temperatures. Overexpression of SmsHSP24.1 significantly enhanced resistance against heat, drought, and salt stresses and showed rapid germination in constitutively overexpressed eggplant lines. RNA-seq analysis reveals an apparent upregulation of a set of reactive oxygen species (ROS) scavenging enzymes of the glutathione (GHS) pathway and mitochondrial electron transport chain (ETC). Significant upregulation was also observed in auxin biosynthesis and cell-wall remodeling transcripts in overexpressed lines. qPCR, biochemical and physiological analysis further aligned with the finding of transcriptome analysis and suggested an essential role of SmsHSP24.1 under various stress responses and positive physiological influence on the growth of eggplants. Therefore, this gene has immense potential in engineering stress-resilient crop plants.Muslima KhatunBhabesh BorphukanIftekhar AlamChaman Ara KeyaVarakumar PanditiHaseena KhanSaaimatul HuqMalireddy K. ReddyMd. SalimullahFrontiers Media S.A.articleeggplantmitochondriachaperonabiotic stressheat tolerancesmall heat shock proteinPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	eggplant mitochondria chaperon abiotic stress heat tolerance small heat shock protein Plant culture SB1-1110
spellingShingle	eggplant mitochondria chaperon abiotic stress heat tolerance small heat shock protein Plant culture SB1-1110 Muslima Khatun Bhabesh Borphukan Iftekhar Alam Chaman Ara Keya Varakumar Panditi Haseena Khan Saaimatul Huq Malireddy K. Reddy Md. Salimullah Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming
description	Among the diverse array of heat shock proteins across the three domains of life, mitochondria-targeted small heat shock proteins (sHSPs) are evolved in the plant lineage. However, they remained mysterious and understudied. In this study, we reported a systematic study of a novel mitochondria-targeted nuclear sHSP from eggplant (Solanum melongena L.; SmsHSP24.1). Differential expression of SmsHSP24.1 indicated its positive role exerted during stress conditions. Escherichia coli-BL21 cell line overexpressing the SmsHSP24.1 showed excellent thermo-tolerance ability, tolerating up to 52°C. Spectrometry and electron microscopy revealed a multimeric structure of the protein which acted as a molecular chaperone at high temperatures. Overexpression of SmsHSP24.1 significantly enhanced resistance against heat, drought, and salt stresses and showed rapid germination in constitutively overexpressed eggplant lines. RNA-seq analysis reveals an apparent upregulation of a set of reactive oxygen species (ROS) scavenging enzymes of the glutathione (GHS) pathway and mitochondrial electron transport chain (ETC). Significant upregulation was also observed in auxin biosynthesis and cell-wall remodeling transcripts in overexpressed lines. qPCR, biochemical and physiological analysis further aligned with the finding of transcriptome analysis and suggested an essential role of SmsHSP24.1 under various stress responses and positive physiological influence on the growth of eggplants. Therefore, this gene has immense potential in engineering stress-resilient crop plants.
format	article
author	Muslima Khatun Bhabesh Borphukan Iftekhar Alam Chaman Ara Keya Varakumar Panditi Haseena Khan Saaimatul Huq Malireddy K. Reddy Md. Salimullah
author_facet	Muslima Khatun Bhabesh Borphukan Iftekhar Alam Chaman Ara Keya Varakumar Panditi Haseena Khan Saaimatul Huq Malireddy K. Reddy Md. Salimullah
author_sort	Muslima Khatun
title	Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming
title_short	Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming
title_full	Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming
title_fullStr	Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming
title_full_unstemmed	Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming
title_sort	mitochondria-targeted smshsp24.1 overexpression stimulates early seedling vigor and stress tolerance by multi-pathway transcriptome-reprogramming
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0209d1218a1c4bf1bbf6f487497e70bc
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Mitochondria-Targeted SmsHSP24.1 Overexpression Stimulates Early Seedling Vigor and Stress Tolerance by Multi-Pathway Transcriptome-Reprogramming

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