Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds

Abstract In tissue engineering, autofluorescence of polymer scaffolds often lowers the image contrast, making it difficult to examine cells and subcellular structures. Treating the scaffold materials with Sudan Black B (SBB) after cell fixation can effectively suppress autofluorescence, but this app...

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Autores principales:	Lin Qi, Erin K. Knapton, Xu Zhang, Tongwen Zhang, Chen Gu, Yi Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/020ae14d0fde4567994e0148b723d231
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spelling	oai:doaj.org-article:020ae14d0fde4567994e0148b723d2312021-12-02T12:32:38ZPre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds10.1038/s41598-017-08723-22045-2322https://doaj.org/article/020ae14d0fde4567994e0148b723d2312017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08723-2https://doaj.org/toc/2045-2322Abstract In tissue engineering, autofluorescence of polymer scaffolds often lowers the image contrast, making it difficult to examine cells and subcellular structures. Treating the scaffold materials with Sudan Black B (SBB) after cell fixation can effectively suppress autofluorescence, but this approach is not conducive to live cell imaging. Post-culture SBB treatment also disrupts intracellular structures and leads to reduced fluorescence intensity of the targets of interest. In this study, we introduce pre-culture SBB treatment to suppress autofluorescence, where SBB is applied to polymeric scaffold materials before cell seeding. The results show that the autofluorescence signals emitted from polycaprolactone (PCL) scaffolds in three commonly used fluorescence channels effectively decrease without diminishing the fluorescence signals emitted from the cells. The pre-culture SBB treatment does not significantly affect cell viability. The autofluorescence suppressive effect does not substantially diminish during the culturing period up to 28 days. The results also show that cell migration, proliferation, and myogenic differentiation in pre-culture SBB-treated groups do not exhibit statistical difference from the non-treated groups. As such, this approach greatly improves the fluorescence image quality for examining live cell behaviors and dynamics while the cells are cultured within autofluorescent polymer scaffolds.Lin QiErin K. KnaptonXu ZhangTongwen ZhangChen GuYi ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Qi Erin K. Knapton Xu Zhang Tongwen Zhang Chen Gu Yi Zhao Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
description	Abstract In tissue engineering, autofluorescence of polymer scaffolds often lowers the image contrast, making it difficult to examine cells and subcellular structures. Treating the scaffold materials with Sudan Black B (SBB) after cell fixation can effectively suppress autofluorescence, but this approach is not conducive to live cell imaging. Post-culture SBB treatment also disrupts intracellular structures and leads to reduced fluorescence intensity of the targets of interest. In this study, we introduce pre-culture SBB treatment to suppress autofluorescence, where SBB is applied to polymeric scaffold materials before cell seeding. The results show that the autofluorescence signals emitted from polycaprolactone (PCL) scaffolds in three commonly used fluorescence channels effectively decrease without diminishing the fluorescence signals emitted from the cells. The pre-culture SBB treatment does not significantly affect cell viability. The autofluorescence suppressive effect does not substantially diminish during the culturing period up to 28 days. The results also show that cell migration, proliferation, and myogenic differentiation in pre-culture SBB-treated groups do not exhibit statistical difference from the non-treated groups. As such, this approach greatly improves the fluorescence image quality for examining live cell behaviors and dynamics while the cells are cultured within autofluorescent polymer scaffolds.
format	article
author	Lin Qi Erin K. Knapton Xu Zhang Tongwen Zhang Chen Gu Yi Zhao
author_facet	Lin Qi Erin K. Knapton Xu Zhang Tongwen Zhang Chen Gu Yi Zhao
author_sort	Lin Qi
title	Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
title_short	Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
title_full	Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
title_fullStr	Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
title_full_unstemmed	Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
title_sort	pre-culture sudan black b treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/020ae14d0fde4567994e0148b723d231
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_version_	1718394017103740928

Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds

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