Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.

Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, meta...

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Autores principales:	Patxi San Martin-Uriz, Salvador Mirete, Pedro J Alcolea, Manuel J Gomez, Ricardo Amils, Jose E Gonzalez-Pastor
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/020e5dc2a3ed47729236e1cdf1a8bd11
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spelling	oai:doaj.org-article:020e5dc2a3ed47729236e1cdf1a8bd112021-11-18T08:22:53ZNickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.1932-620310.1371/journal.pone.0095041https://doaj.org/article/020e5dc2a3ed47729236e1cdf1a8bd112014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24740277/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, metal-laden river located in southwestern Spain. The characterization of its metal resistance revealed a remarkable ability to tolerate high Ni concentrations. Here we report the screening of a genomic library of Acidiphilium sp. PM to identify genes involved in Ni resistance. This approach revealed seven different genes conferring Ni resistance to E. coli, two of which form an operon encoding the ATP-dependent protease HslVU (ClpQY). This protease was found to enhance resistance to both Ni and Co in E. coli, a function not previously reported. Other Ni-resistance determinants include genes involved in lipopolysaccharide biosynthesis and the synthesis of branched amino acids. The diversity of molecular functions of the genes recovered in the screening suggests that Ni resistance in Acidiphilium sp. PM probably relies on different molecular mechanisms.Patxi San Martin-UrizSalvador MiretePedro J AlcoleaManuel J GomezRicardo AmilsJose E Gonzalez-PastorPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e95041 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patxi San Martin-Uriz Salvador Mirete Pedro J Alcolea Manuel J Gomez Ricardo Amils Jose E Gonzalez-Pastor Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
description	Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, metal-laden river located in southwestern Spain. The characterization of its metal resistance revealed a remarkable ability to tolerate high Ni concentrations. Here we report the screening of a genomic library of Acidiphilium sp. PM to identify genes involved in Ni resistance. This approach revealed seven different genes conferring Ni resistance to E. coli, two of which form an operon encoding the ATP-dependent protease HslVU (ClpQY). This protease was found to enhance resistance to both Ni and Co in E. coli, a function not previously reported. Other Ni-resistance determinants include genes involved in lipopolysaccharide biosynthesis and the synthesis of branched amino acids. The diversity of molecular functions of the genes recovered in the screening suggests that Ni resistance in Acidiphilium sp. PM probably relies on different molecular mechanisms.
format	article
author	Patxi San Martin-Uriz Salvador Mirete Pedro J Alcolea Manuel J Gomez Ricardo Amils Jose E Gonzalez-Pastor
author_facet	Patxi San Martin-Uriz Salvador Mirete Pedro J Alcolea Manuel J Gomez Ricardo Amils Jose E Gonzalez-Pastor
author_sort	Patxi San Martin-Uriz
title	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
title_short	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
title_full	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
title_fullStr	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
title_full_unstemmed	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.
title_sort	nickel-resistance determinants in acidiphilium sp. pm identified by genome-wide functional screening.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/020e5dc2a3ed47729236e1cdf1a8bd11
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Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.

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