Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India

Abstract Although skin is the primary affected organ in Leprosy, the role of the skin microbiome in its pathogenesis is not well understood. Recent reports have shown that skin of leprosy patients (LP) harbours perturbed microbiota which grants inflammation and disease progression. Herein, we presen...

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Autores principales:	Nitin Bayal, Sunil Nagpal, Mohammed Monzoorul Haque, Milind S. Patole, Yogesh Shouche, Shekhar C. Mande, Sharmila S. Mande
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0210b6b4b58d4191b4181edecd189966
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spelling	oai:doaj.org-article:0210b6b4b58d4191b4181edecd1899662021-12-02T14:26:54ZStructural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India10.1038/s41598-020-80533-52045-2322https://doaj.org/article/0210b6b4b58d4191b4181edecd1899662021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80533-5https://doaj.org/toc/2045-2322Abstract Although skin is the primary affected organ in Leprosy, the role of the skin microbiome in its pathogenesis is not well understood. Recent reports have shown that skin of leprosy patients (LP) harbours perturbed microbiota which grants inflammation and disease progression. Herein, we present the results of nested Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) which was initially performed for investigating the diversity of bacterial communities from lesional skin (LS) and non-lesional skin (NLS) sites of LP (n = 11). Further, we performed comprehensive analysis of 16S rRNA profiles corresponding to skin samples from participants (n = 90) located in two geographical locations i.e. Hyderabad and Miraj in India. The genus Staphylococcus was observed to be one of the representative bacteria characterizing healthy controls (HC; n = 30), which in contrast was underrepresented in skin microbiota of LP. Taxa affiliated to phyla Firmicutes and Proteobacteria were found to be signatures of HC and LS, respectively. Observed diversity level changes, shifts in core microbiota, and community network structure support the evident dysbiosis in normal skin microbiota due to leprosy. Insights obtained indicate the need for exploring skin microbiota modulation as a potential therapeutic option for leprosy.Nitin BayalSunil NagpalMohammed Monzoorul HaqueMilind S. PatoleYogesh ShoucheShekhar C. MandeSharmila S. MandeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nitin Bayal Sunil Nagpal Mohammed Monzoorul Haque Milind S. Patole Yogesh Shouche Shekhar C. Mande Sharmila S. Mande Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India
description	Abstract Although skin is the primary affected organ in Leprosy, the role of the skin microbiome in its pathogenesis is not well understood. Recent reports have shown that skin of leprosy patients (LP) harbours perturbed microbiota which grants inflammation and disease progression. Herein, we present the results of nested Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) which was initially performed for investigating the diversity of bacterial communities from lesional skin (LS) and non-lesional skin (NLS) sites of LP (n = 11). Further, we performed comprehensive analysis of 16S rRNA profiles corresponding to skin samples from participants (n = 90) located in two geographical locations i.e. Hyderabad and Miraj in India. The genus Staphylococcus was observed to be one of the representative bacteria characterizing healthy controls (HC; n = 30), which in contrast was underrepresented in skin microbiota of LP. Taxa affiliated to phyla Firmicutes and Proteobacteria were found to be signatures of HC and LS, respectively. Observed diversity level changes, shifts in core microbiota, and community network structure support the evident dysbiosis in normal skin microbiota due to leprosy. Insights obtained indicate the need for exploring skin microbiota modulation as a potential therapeutic option for leprosy.
format	article
author	Nitin Bayal Sunil Nagpal Mohammed Monzoorul Haque Milind S. Patole Yogesh Shouche Shekhar C. Mande Sharmila S. Mande
author_facet	Nitin Bayal Sunil Nagpal Mohammed Monzoorul Haque Milind S. Patole Yogesh Shouche Shekhar C. Mande Sharmila S. Mande
author_sort	Nitin Bayal
title	Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India
title_short	Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India
title_full	Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India
title_fullStr	Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India
title_full_unstemmed	Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India
title_sort	structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from india
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0210b6b4b58d4191b4181edecd189966
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Structural aspects of lesional and non-lesional skin microbiota reveal key community changes in leprosy patients from India

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