Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies

Abstract To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes...

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Autores principales: Ivana Domljanovic, Annika Carstens, Anders Okholm, Jørgen Kjems, Christoffer Tandrup Nielsen, Niels H. H. Heegaard, Kira Astakhova
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/021733dead7c4bbd84e9d816ea1b535a
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spelling oai:doaj.org-article:021733dead7c4bbd84e9d816ea1b535a2021-12-02T12:30:11ZComplexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies10.1038/s41598-017-02214-02045-2322https://doaj.org/article/021733dead7c4bbd84e9d816ea1b535a2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02214-0https://doaj.org/toc/2045-2322Abstract To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes as targets for these antibodies. This is done in a simple, rapid and specific immunofluorescence assay. Specifically, employing 3D nanostructures (DNA origami), we present a new approach in the detection and study of human antibodies to DNA. We demonstrate the detection of anti-DNA antibodies that are characteristic of systemic lupus erythematosus, a chronic autoimmune disease with multiple manifestations. We tested the most potent non-covalent pairs of DNA and fluorescent dyes. Several complexes showed specific recognition of autoimmune antibodies in human samples of lupus patients using a simple one-step immunofluorescence method. This makes the novel assay developed herein a promising tool for research and point-of-care monitoring of anti-DNA antibodies. Using this method, we for the first time experimentally confirm that the disease-specific autoimmune antibodies are sensitive to the 3D structure of nucleic acids and not only to the nucleotide sequence, as was previously thought.Ivana DomljanovicAnnika CarstensAnders OkholmJørgen KjemsChristoffer Tandrup NielsenNiels H. H. HeegaardKira AstakhovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ivana Domljanovic
Annika Carstens
Anders Okholm
Jørgen Kjems
Christoffer Tandrup Nielsen
Niels H. H. Heegaard
Kira Astakhova
Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
description Abstract To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes as targets for these antibodies. This is done in a simple, rapid and specific immunofluorescence assay. Specifically, employing 3D nanostructures (DNA origami), we present a new approach in the detection and study of human antibodies to DNA. We demonstrate the detection of anti-DNA antibodies that are characteristic of systemic lupus erythematosus, a chronic autoimmune disease with multiple manifestations. We tested the most potent non-covalent pairs of DNA and fluorescent dyes. Several complexes showed specific recognition of autoimmune antibodies in human samples of lupus patients using a simple one-step immunofluorescence method. This makes the novel assay developed herein a promising tool for research and point-of-care monitoring of anti-DNA antibodies. Using this method, we for the first time experimentally confirm that the disease-specific autoimmune antibodies are sensitive to the 3D structure of nucleic acids and not only to the nucleotide sequence, as was previously thought.
format article
author Ivana Domljanovic
Annika Carstens
Anders Okholm
Jørgen Kjems
Christoffer Tandrup Nielsen
Niels H. H. Heegaard
Kira Astakhova
author_facet Ivana Domljanovic
Annika Carstens
Anders Okholm
Jørgen Kjems
Christoffer Tandrup Nielsen
Niels H. H. Heegaard
Kira Astakhova
author_sort Ivana Domljanovic
title Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
title_short Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
title_full Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
title_fullStr Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
title_full_unstemmed Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
title_sort complexes of dna with fluorescent dyes are effective reagents for detection of autoimmune antibodies
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/021733dead7c4bbd84e9d816ea1b535a
work_keys_str_mv AT ivanadomljanovic complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
AT annikacarstens complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
AT andersokholm complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
AT jørgenkjems complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
AT christoffertandrupnielsen complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
AT nielshhheegaard complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
AT kiraastakhova complexesofdnawithfluorescentdyesareeffectivereagentsfordetectionofautoimmuneantibodies
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