Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.

One of the major risk factors for head and neck squamous cell carcinoma (HNSCC) is tobacco smoke exposure, but the mechanisms that can account for disease development remain to be fully defined. Utilizing our HNSCC mouse model, we analyzed oral squamous cell carcinomas (OSCC) induced by the active m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Douglas B Stairs, Mary E Landmesser, Cesar Aliaga, Kun-Ming Chen, Yuan-Wan Sun, Karam El-Bayoumy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02182b31ee9b40d995705b76d5196d3c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:02182b31ee9b40d995705b76d5196d3c
record_format	dspace
spelling	oai:doaj.org-article:02182b31ee9b40d995705b76d5196d3c2021-12-02T20:12:59ZBlack raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.1932-620310.1371/journal.pone.0259998https://doaj.org/article/02182b31ee9b40d995705b76d5196d3c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259998https://doaj.org/toc/1932-6203One of the major risk factors for head and neck squamous cell carcinoma (HNSCC) is tobacco smoke exposure, but the mechanisms that can account for disease development remain to be fully defined. Utilizing our HNSCC mouse model, we analyzed oral squamous cell carcinomas (OSCC) induced by the active metabolite of a common smoke constituent, dibenzo[a,l]pyrene diol-epoxide (DBPDE). Analyzing protein expression by either immunofluorescence or immunohistochemistry, we identified biologic processes that are dysregulated in premalignant and invasive cancer lesions induced by DBPDE. Interestingly, p120ctn expression is downregulated in both stages of the disease. In addition to decreased p120ctn expression, there was also increased proliferation (as measured by Ki67), inflammation (as measured by NFkB (p65) expression), neovascularization (as measured by CD31) and recruitment of Ly6G-positive immune cells as well as strong EGFR expression. We also examined the effect of the chemopreventive agent black raspberry (BRB) on p120ctn and EGFR protein expression in DBPDE treated mice. p120ctn, but not EGFR, protein expression increased in mice treated with BRB. Our results suggest that modulation of p120ctn may, in part, account for the mechanism by which BRB inhibits DBPDE induced OSCC in mice.Douglas B StairsMary E LandmesserCesar AliagaKun-Ming ChenYuan-Wan SunKaram El-BayoumyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259998 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Douglas B Stairs Mary E Landmesser Cesar Aliaga Kun-Ming Chen Yuan-Wan Sun Karam El-Bayoumy Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
description	One of the major risk factors for head and neck squamous cell carcinoma (HNSCC) is tobacco smoke exposure, but the mechanisms that can account for disease development remain to be fully defined. Utilizing our HNSCC mouse model, we analyzed oral squamous cell carcinomas (OSCC) induced by the active metabolite of a common smoke constituent, dibenzo[a,l]pyrene diol-epoxide (DBPDE). Analyzing protein expression by either immunofluorescence or immunohistochemistry, we identified biologic processes that are dysregulated in premalignant and invasive cancer lesions induced by DBPDE. Interestingly, p120ctn expression is downregulated in both stages of the disease. In addition to decreased p120ctn expression, there was also increased proliferation (as measured by Ki67), inflammation (as measured by NFkB (p65) expression), neovascularization (as measured by CD31) and recruitment of Ly6G-positive immune cells as well as strong EGFR expression. We also examined the effect of the chemopreventive agent black raspberry (BRB) on p120ctn and EGFR protein expression in DBPDE treated mice. p120ctn, but not EGFR, protein expression increased in mice treated with BRB. Our results suggest that modulation of p120ctn may, in part, account for the mechanism by which BRB inhibits DBPDE induced OSCC in mice.
format	article
author	Douglas B Stairs Mary E Landmesser Cesar Aliaga Kun-Ming Chen Yuan-Wan Sun Karam El-Bayoumy
author_facet	Douglas B Stairs Mary E Landmesser Cesar Aliaga Kun-Ming Chen Yuan-Wan Sun Karam El-Bayoumy
author_sort	Douglas B Stairs
title	Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
title_short	Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
title_full	Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
title_fullStr	Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
title_full_unstemmed	Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
title_sort	black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/02182b31ee9b40d995705b76d5196d3c
work_keys_str_mv	AT douglasbstairs blackraspberryrestorestheexpressionofthetumorsuppressorp120ctnintheoralcavityofmicetreatedwiththecarcinogendibenzoalpyrenediolepoxide AT maryelandmesser blackraspberryrestorestheexpressionofthetumorsuppressorp120ctnintheoralcavityofmicetreatedwiththecarcinogendibenzoalpyrenediolepoxide AT cesaraliaga blackraspberryrestorestheexpressionofthetumorsuppressorp120ctnintheoralcavityofmicetreatedwiththecarcinogendibenzoalpyrenediolepoxide AT kunmingchen blackraspberryrestorestheexpressionofthetumorsuppressorp120ctnintheoralcavityofmicetreatedwiththecarcinogendibenzoalpyrenediolepoxide AT yuanwansun blackraspberryrestorestheexpressionofthetumorsuppressorp120ctnintheoralcavityofmicetreatedwiththecarcinogendibenzoalpyrenediolepoxide AT karamelbayoumy blackraspberryrestorestheexpressionofthetumorsuppressorp120ctnintheoralcavityofmicetreatedwiththecarcinogendibenzoalpyrenediolepoxide
_version_	1718374803715391488

Black raspberry restores the expression of the tumor suppressor p120ctn in the oral cavity of mice treated with the carcinogen dibenzo[a,l]pyrene diol epoxide.

Ejemplares similares