Foxm1 controls a pro-stemness microRNA network in neural stem cells

Abstract Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA express...

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Autores principales:	Zein Mersini Besharat, Luana Abballe, Francesco Cicconardi, Arjun Bhutkar, Luigi Grassi, Loredana Le Pera, Marta Moretti, Mauro Chinappi, Daniel D’Andrea, Angela Mastronuzzi, Alessandra Ianari, Alessandra Vacca, Enrico De Smaele, Franco Locatelli, Agnese Po, Evelina Miele, Elisabetta Ferretti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/021c39da592f4fb1a9f27206e7091f9d
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spelling	oai:doaj.org-article:021c39da592f4fb1a9f27206e7091f9d2021-12-02T16:07:51ZFoxm1 controls a pro-stemness microRNA network in neural stem cells10.1038/s41598-018-21876-y2045-2322https://doaj.org/article/021c39da592f4fb1a9f27206e7091f9d2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21876-yhttps://doaj.org/toc/2045-2322Abstract Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in cerebellar NSCs, before and after induced differentiation (Diff-NSCs). Genes with higher transcript levels in NSCs (vs. Diff-NSCs) included Foxm1, which proved to be directly regulated by Gli and Nanog. Foxm1 in turn regulated several microRNAs that were overexpressed in NSCs: miR-130b, miR-301a, and members of the miR-15~16 and miR-17~92 clusters and whose knockdown significantly impaired the neurosphere formation ability. Our results reveal a novel Hh-Gli-Nanog-driven Foxm1-microRNA network that controls the self-renewal capacity of NSCs.Zein Mersini BesharatLuana AbballeFrancesco CicconardiArjun BhutkarLuigi GrassiLoredana Le PeraMarta MorettiMauro ChinappiDaniel D’AndreaAngela MastronuzziAlessandra IanariAlessandra VaccaEnrico De SmaeleFranco LocatelliAgnese PoEvelina MieleElisabetta FerrettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zein Mersini Besharat Luana Abballe Francesco Cicconardi Arjun Bhutkar Luigi Grassi Loredana Le Pera Marta Moretti Mauro Chinappi Daniel D’Andrea Angela Mastronuzzi Alessandra Ianari Alessandra Vacca Enrico De Smaele Franco Locatelli Agnese Po Evelina Miele Elisabetta Ferretti Foxm1 controls a pro-stemness microRNA network in neural stem cells
description	Abstract Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in cerebellar NSCs, before and after induced differentiation (Diff-NSCs). Genes with higher transcript levels in NSCs (vs. Diff-NSCs) included Foxm1, which proved to be directly regulated by Gli and Nanog. Foxm1 in turn regulated several microRNAs that were overexpressed in NSCs: miR-130b, miR-301a, and members of the miR-15~16 and miR-17~92 clusters and whose knockdown significantly impaired the neurosphere formation ability. Our results reveal a novel Hh-Gli-Nanog-driven Foxm1-microRNA network that controls the self-renewal capacity of NSCs.
format	article
author	Zein Mersini Besharat Luana Abballe Francesco Cicconardi Arjun Bhutkar Luigi Grassi Loredana Le Pera Marta Moretti Mauro Chinappi Daniel D’Andrea Angela Mastronuzzi Alessandra Ianari Alessandra Vacca Enrico De Smaele Franco Locatelli Agnese Po Evelina Miele Elisabetta Ferretti
author_facet	Zein Mersini Besharat Luana Abballe Francesco Cicconardi Arjun Bhutkar Luigi Grassi Loredana Le Pera Marta Moretti Mauro Chinappi Daniel D’Andrea Angela Mastronuzzi Alessandra Ianari Alessandra Vacca Enrico De Smaele Franco Locatelli Agnese Po Evelina Miele Elisabetta Ferretti
author_sort	Zein Mersini Besharat
title	Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_short	Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_full	Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_fullStr	Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_full_unstemmed	Foxm1 controls a pro-stemness microRNA network in neural stem cells
title_sort	foxm1 controls a pro-stemness microrna network in neural stem cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/021c39da592f4fb1a9f27206e7091f9d
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Foxm1 controls a pro-stemness microRNA network in neural stem cells

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