<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription

Many positive-sense RNA viruses transcribe subgenomic (sg) mRNAs during infections that template the translation of a subset of viral proteins. Red clover necrotic mosaic virus (RCNMV) expresses its capsid protein through the transcription of a sg mRNA from RNA1 genome segment. This transcription ev...

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Autores principales:	Jennifer S. H. Im, Laura R. Newburn, Gregory Kent, K. Andrew White
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	RNA virus plant virus <i>Tombusviridae</i> tombusvirus dianthovirus RNA–RNA Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/02225d4429214cc2808e812a4d30513b
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spelling	oai:doaj.org-article:02225d4429214cc2808e812a4d30513b2021-11-25T19:13:47Z<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription10.3390/v131122521999-4915https://doaj.org/article/02225d4429214cc2808e812a4d30513b2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2252https://doaj.org/toc/1999-4915Many positive-sense RNA viruses transcribe subgenomic (sg) mRNAs during infections that template the translation of a subset of viral proteins. Red clover necrotic mosaic virus (RCNMV) expresses its capsid protein through the transcription of a sg mRNA from RNA1 genome segment. This transcription event is activated by an RNA structure formed by base pairing between a <i>trans</i>-activator (TA) in RNA2 and a <i>trans</i>-activator binding site (TABS) in RNA1. In this study, the impact of the structural context of the TABS in RNA1 on the TA–TABS interaction and sg mRNA transcription was investigated using in vitro and in vivo approaches. The results (i) generated RNA secondary structure models for the TA and TABS, (ii) revealed that the TABS is partially base paired with proximal upstream sequences, which limits TA access, (iii) demonstrated that the aforementioned intra-RNA1 base pairing involving the TABS modulates the TA–TABS interaction in vitro and sg mRNA levels during infections, and (iv) revealed that the TABS in RNA1 can be modified to mediate sg mRNA transcription in a TA-independent manner. These findings advance our understanding of transcriptional regulation in RCNMV and provide novel insights into the origin of the TA–TABS interaction.Jennifer S. H. ImLaura R. NewburnGregory KentK. Andrew WhiteMDPI AGarticleRNA virusplant virus<i>Tombusviridae</i>tombusvirusdianthovirusRNA–RNAMicrobiologyQR1-502ENViruses, Vol 13, Iss 2252, p 2252 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA virus plant virus <i>Tombusviridae</i> tombusvirus dianthovirus RNA–RNA Microbiology QR1-502
spellingShingle	RNA virus plant virus <i>Tombusviridae</i> tombusvirus dianthovirus RNA–RNA Microbiology QR1-502 Jennifer S. H. Im Laura R. Newburn Gregory Kent K. Andrew White <i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription
description	Many positive-sense RNA viruses transcribe subgenomic (sg) mRNAs during infections that template the translation of a subset of viral proteins. Red clover necrotic mosaic virus (RCNMV) expresses its capsid protein through the transcription of a sg mRNA from RNA1 genome segment. This transcription event is activated by an RNA structure formed by base pairing between a <i>trans</i>-activator (TA) in RNA2 and a <i>trans</i>-activator binding site (TABS) in RNA1. In this study, the impact of the structural context of the TABS in RNA1 on the TA–TABS interaction and sg mRNA transcription was investigated using in vitro and in vivo approaches. The results (i) generated RNA secondary structure models for the TA and TABS, (ii) revealed that the TABS is partially base paired with proximal upstream sequences, which limits TA access, (iii) demonstrated that the aforementioned intra-RNA1 base pairing involving the TABS modulates the TA–TABS interaction in vitro and sg mRNA levels during infections, and (iv) revealed that the TABS in RNA1 can be modified to mediate sg mRNA transcription in a TA-independent manner. These findings advance our understanding of transcriptional regulation in RCNMV and provide novel insights into the origin of the TA–TABS interaction.
format	article
author	Jennifer S. H. Im Laura R. Newburn Gregory Kent K. Andrew White
author_facet	Jennifer S. H. Im Laura R. Newburn Gregory Kent K. Andrew White
author_sort	Jennifer S. H. Im
title	<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription
title_short	<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription
title_full	<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription
title_fullStr	<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription
title_full_unstemmed	<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription
title_sort	<i>trans</i>-activator binding site context in rcnmv modulates subgenomic mrna transcription
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/02225d4429214cc2808e812a4d30513b
work_keys_str_mv	AT jennifershim itransiactivatorbindingsitecontextinrcnmvmodulatessubgenomicmrnatranscription AT laurarnewburn itransiactivatorbindingsitecontextinrcnmvmodulatessubgenomicmrnatranscription AT gregorykent itransiactivatorbindingsitecontextinrcnmvmodulatessubgenomicmrnatranscription AT kandrewwhite itransiactivatorbindingsitecontextinrcnmvmodulatessubgenomicmrnatranscription
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<i>Trans</i>-Activator Binding Site Context in RCNMV Modulates Subgenomic mRNA Transcription

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