Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV

ABSTRACT Breast milk is nutritionally and immunologically beneficial in early life but is also a potential source of infection. Little is known about breast milk microbiota of women living with HIV (WLHIV), the impact of severe immunosuppression, and the contribution to mortality of HIV-exposed infa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rabia Maqsood, Joshua B. Reus, Lily I. Wu, LaRinda A. Holland, Ruth Nduati, Dorothy Mbori-Ngacha, Elizabeth Maleche-Obimbo, Emily R. Begnel, Soren Gantt, Ednah Ojee, Dalton Wamalwa, Grace John-Stewart, Jennifer Slyker, Dara A. Lehman, Efrem S. Lim
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	breast milk CMV HIV microbiome virome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/022bd06882e74eaeb55281ea539000b0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:022bd06882e74eaeb55281ea539000b0
record_format	dspace
spelling	oai:doaj.org-article:022bd06882e74eaeb55281ea539000b02021-12-02T17:07:47ZBreast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV10.1128/mSystems.01079-202379-5077https://doaj.org/article/022bd06882e74eaeb55281ea539000b02021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01079-20https://doaj.org/toc/2379-5077ABSTRACT Breast milk is nutritionally and immunologically beneficial in early life but is also a potential source of infection. Little is known about breast milk microbiota of women living with HIV (WLHIV), the impact of severe immunosuppression, and the contribution to mortality of HIV-exposed infants. Here, we performed metagenomic sequencing to characterize the bacterial microbiome and DNA virome of breast milk samples at 1 month postpartum from Kenyan WLHIV who were not receiving combination antiretroviral therapy (cART), 23 women with CD4 counts of <250 and 30 women with CD4 of >500; and additionally, 19 WLHIV with infants that lived and 26 WLHIV with infants that died during the first 2 years of life were included. We found that breast milk bacterial microbiomes in this study population were highly diverse but shared a core community composed of the Streptococcaceae, Staphylococcaceae, Moraxellaceae, and Eubacteriaceae families. The breast milk virome was dominated by human cytomegalovirus (CMV) and included the bacteriophage families Myoviridae, Siphoviridae, and Podoviridae. Bacterial microbiome and virome profiles and diversity were not significantly altered by HIV immunosuppression, as defined by a CD4 of <250. CMV viral load was not associated with maternal CD4 counts or infant mortality. In conclusion, we show that the core bacterial and viral communities are resilient in breast milk despite immunosuppression in WLHIV. IMPORTANCE Breastfeeding plays an important role in seeding the infant gut microbiome and mammary health. Although most studies focus on the diverse breast milk bacterial communities, little is known about the viral communities harbored in breast milk. We performed the first breast milk virome study of an HIV population. In this study cohort of Kenyan women living with HIV from the pre-antiretroviral therapy era, we found that breast milk harbors a core bacterial microbiome and a virome dominated by human cytomegalovirus. The virome and bacterial microbiome were not substantially altered by immunosuppression or associated with infant mortality. Together, these findings indicate resilience of the microbial community in breast milk compartmentalization. These findings advance out fundamental understanding of the breast milk core microbiome and virome interactions in the context of HIV disease.Rabia MaqsoodJoshua B. ReusLily I. WuLaRinda A. HollandRuth NduatiDorothy Mbori-NgachaElizabeth Maleche-ObimboEmily R. BegnelSoren GanttEdnah OjeeDalton WamalwaGrace John-StewartJennifer SlykerDara A. LehmanEfrem S. LimAmerican Society for Microbiologyarticlebreast milkCMVHIVmicrobiomeviromeMicrobiologyQR1-502ENmSystems, Vol 6, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast milk CMV HIV microbiome virome Microbiology QR1-502
spellingShingle	breast milk CMV HIV microbiome virome Microbiology QR1-502 Rabia Maqsood Joshua B. Reus Lily I. Wu LaRinda A. Holland Ruth Nduati Dorothy Mbori-Ngacha Elizabeth Maleche-Obimbo Emily R. Begnel Soren Gantt Ednah Ojee Dalton Wamalwa Grace John-Stewart Jennifer Slyker Dara A. Lehman Efrem S. Lim Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV
description	ABSTRACT Breast milk is nutritionally and immunologically beneficial in early life but is also a potential source of infection. Little is known about breast milk microbiota of women living with HIV (WLHIV), the impact of severe immunosuppression, and the contribution to mortality of HIV-exposed infants. Here, we performed metagenomic sequencing to characterize the bacterial microbiome and DNA virome of breast milk samples at 1 month postpartum from Kenyan WLHIV who were not receiving combination antiretroviral therapy (cART), 23 women with CD4 counts of <250 and 30 women with CD4 of >500; and additionally, 19 WLHIV with infants that lived and 26 WLHIV with infants that died during the first 2 years of life were included. We found that breast milk bacterial microbiomes in this study population were highly diverse but shared a core community composed of the Streptococcaceae, Staphylococcaceae, Moraxellaceae, and Eubacteriaceae families. The breast milk virome was dominated by human cytomegalovirus (CMV) and included the bacteriophage families Myoviridae, Siphoviridae, and Podoviridae. Bacterial microbiome and virome profiles and diversity were not significantly altered by HIV immunosuppression, as defined by a CD4 of <250. CMV viral load was not associated with maternal CD4 counts or infant mortality. In conclusion, we show that the core bacterial and viral communities are resilient in breast milk despite immunosuppression in WLHIV. IMPORTANCE Breastfeeding plays an important role in seeding the infant gut microbiome and mammary health. Although most studies focus on the diverse breast milk bacterial communities, little is known about the viral communities harbored in breast milk. We performed the first breast milk virome study of an HIV population. In this study cohort of Kenyan women living with HIV from the pre-antiretroviral therapy era, we found that breast milk harbors a core bacterial microbiome and a virome dominated by human cytomegalovirus. The virome and bacterial microbiome were not substantially altered by immunosuppression or associated with infant mortality. Together, these findings indicate resilience of the microbial community in breast milk compartmentalization. These findings advance out fundamental understanding of the breast milk core microbiome and virome interactions in the context of HIV disease.
format	article
author	Rabia Maqsood Joshua B. Reus Lily I. Wu LaRinda A. Holland Ruth Nduati Dorothy Mbori-Ngacha Elizabeth Maleche-Obimbo Emily R. Begnel Soren Gantt Ednah Ojee Dalton Wamalwa Grace John-Stewart Jennifer Slyker Dara A. Lehman Efrem S. Lim
author_facet	Rabia Maqsood Joshua B. Reus Lily I. Wu LaRinda A. Holland Ruth Nduati Dorothy Mbori-Ngacha Elizabeth Maleche-Obimbo Emily R. Begnel Soren Gantt Ednah Ojee Dalton Wamalwa Grace John-Stewart Jennifer Slyker Dara A. Lehman Efrem S. Lim
author_sort	Rabia Maqsood
title	Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV
title_short	Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV
title_full	Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV
title_fullStr	Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV
title_full_unstemmed	Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV
title_sort	breast milk virome and bacterial microbiome resilience in kenyan women living with hiv
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/022bd06882e74eaeb55281ea539000b0
work_keys_str_mv	AT rabiamaqsood breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT joshuabreus breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT lilyiwu breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT larindaaholland breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT ruthnduati breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT dorothymboringacha breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT elizabethmalecheobimbo breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT emilyrbegnel breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT sorengantt breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT ednahojee breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT daltonwamalwa breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT gracejohnstewart breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT jenniferslyker breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT daraalehman breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv AT efremslim breastmilkviromeandbacterialmicrobiomeresilienceinkenyanwomenlivingwithhiv
_version_	1718381535098306560

Breast Milk Virome and Bacterial Microbiome Resilience in Kenyan Women Living with HIV

Ejemplares similares