Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens

Abstract Under certain circumstances differentiated cells can be reprogrammed to form stem cells in land plants, but only a portion of the cells reprograms successfully. A long-distance inhibitory signal from reprogrammed cells to surrounding cells has been reported in some ferns. Here we show the e...

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Autores principales:	Yoshikatsu Sato, Nagisa Sugimoto, Tadayoshi Hirai, Akihiro Imai, Minoru Kubo, Yuji Hiwatashi, Tomoaki Nishiyama, Mitsuyasu Hasebe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/023cc627e9084d43aee694ba331d0bfb
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spelling	oai:doaj.org-article:023cc627e9084d43aee694ba331d0bfb2021-12-02T11:52:58ZCells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens10.1038/s41598-017-01786-12045-2322https://doaj.org/article/023cc627e9084d43aee694ba331d0bfb2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01786-1https://doaj.org/toc/2045-2322Abstract Under certain circumstances differentiated cells can be reprogrammed to form stem cells in land plants, but only a portion of the cells reprograms successfully. A long-distance inhibitory signal from reprogrammed cells to surrounding cells has been reported in some ferns. Here we show the existence of anisotropic inhibitory signal to regulate stem cell formation in the moss Physcomitrella patens. When single cells were isolated from a gametophore leaf, over 90% of them were reprogrammed to stem cells with characteristic nuclear expansion. By contrast, when two adjacent cells were isolated, the nuclei of both cells expanded, but successful reprogramming of both cells occurred only in approximately one fifth of the pairs. When three aligned cells were isolated, the reprogramming rate of both edge cells decreased with a living middle cell but did not with a dead middle cell. Furthermore, unequal conversion into stem cells was more prominent in cell pairs aligned parallel to the proximal-distal leaf axis than in those perpendicular to the axis. This study gives an insight into the role of the inhibitory signal in development and evolution as well as the efficient stem cell induction from differentiated cells.Yoshikatsu SatoNagisa SugimotoTadayoshi HiraiAkihiro ImaiMinoru KuboYuji HiwatashiTomoaki NishiyamaMitsuyasu HasebeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshikatsu Sato Nagisa Sugimoto Tadayoshi Hirai Akihiro Imai Minoru Kubo Yuji Hiwatashi Tomoaki Nishiyama Mitsuyasu Hasebe Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
description	Abstract Under certain circumstances differentiated cells can be reprogrammed to form stem cells in land plants, but only a portion of the cells reprograms successfully. A long-distance inhibitory signal from reprogrammed cells to surrounding cells has been reported in some ferns. Here we show the existence of anisotropic inhibitory signal to regulate stem cell formation in the moss Physcomitrella patens. When single cells were isolated from a gametophore leaf, over 90% of them were reprogrammed to stem cells with characteristic nuclear expansion. By contrast, when two adjacent cells were isolated, the nuclei of both cells expanded, but successful reprogramming of both cells occurred only in approximately one fifth of the pairs. When three aligned cells were isolated, the reprogramming rate of both edge cells decreased with a living middle cell but did not with a dead middle cell. Furthermore, unequal conversion into stem cells was more prominent in cell pairs aligned parallel to the proximal-distal leaf axis than in those perpendicular to the axis. This study gives an insight into the role of the inhibitory signal in development and evolution as well as the efficient stem cell induction from differentiated cells.
format	article
author	Yoshikatsu Sato Nagisa Sugimoto Tadayoshi Hirai Akihiro Imai Minoru Kubo Yuji Hiwatashi Tomoaki Nishiyama Mitsuyasu Hasebe
author_facet	Yoshikatsu Sato Nagisa Sugimoto Tadayoshi Hirai Akihiro Imai Minoru Kubo Yuji Hiwatashi Tomoaki Nishiyama Mitsuyasu Hasebe
author_sort	Yoshikatsu Sato
title	Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
title_short	Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
title_full	Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
title_fullStr	Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
title_full_unstemmed	Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens
title_sort	cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss physcomitrella patens
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/023cc627e9084d43aee694ba331d0bfb
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Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens

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