Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients

Abstract Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicolas Lanthier, Julie Rodriguez, Maxime Nachit, Sophie Hiel, Pierre Trefois, Audrey M. Neyrinck, Patrice D. Cani, Laure B. Bindels, Jean-Paul Thissen, Nathalie M. Delzenne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/023ec02cb2dd45cc98da54f66da34aec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:023ec02cb2dd45cc98da54f66da34aec
record_format	dspace
spelling	oai:doaj.org-article:023ec02cb2dd45cc98da54f66da34aec2021-12-02T15:23:08ZMicrobiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients10.1038/s41598-020-79718-92045-2322https://doaj.org/article/023ec02cb2dd45cc98da54f66da34aec2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79718-9https://doaj.org/toc/2045-2322Abstract Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe steatosis (n = 36) had higher ALAT values, fasting blood glucose levels as well as higher visceral adipose tissue area and skeletal muscle index evaluated by computed tomography. Patients with fibrosis (n = 13) had higher ASAT values, increased whole muscle area and lower skeletal muscle density index. In a multivariate logistic regression analysis, myosteatosis was the strongest factor associated with fibrosis. Illumina sequencing of 16S rRNA gene amplicon was performed on fecal samples. The relative abundance of fecal Clostridium sensu stricto was significantly decreased with the presence of liver fibrosis and was negatively associated with liver stiffness measurement and myosteatosis. In addition, 19 amplicon sequence variants were regulated according to the severity of the disease. Linear discriminant analysis effect size (LEfSe) also highlighted discriminant microbes in patients with fibrosis, such as an enrichment of Enterobacteriaceae and Escherichia/Shigella compared to patients with severe steatosis without fibrosis. All those data suggest a gut-liver-muscle axis in the pathogenesis of MAFLD complications.Nicolas LanthierJulie RodriguezMaxime NachitSophie HielPierre TrefoisAudrey M. NeyrinckPatrice D. CaniLaure B. BindelsJean-Paul ThissenNathalie M. DelzenneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicolas Lanthier Julie Rodriguez Maxime Nachit Sophie Hiel Pierre Trefois Audrey M. Neyrinck Patrice D. Cani Laure B. Bindels Jean-Paul Thissen Nathalie M. Delzenne Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
description	Abstract Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe steatosis (n = 36) had higher ALAT values, fasting blood glucose levels as well as higher visceral adipose tissue area and skeletal muscle index evaluated by computed tomography. Patients with fibrosis (n = 13) had higher ASAT values, increased whole muscle area and lower skeletal muscle density index. In a multivariate logistic regression analysis, myosteatosis was the strongest factor associated with fibrosis. Illumina sequencing of 16S rRNA gene amplicon was performed on fecal samples. The relative abundance of fecal Clostridium sensu stricto was significantly decreased with the presence of liver fibrosis and was negatively associated with liver stiffness measurement and myosteatosis. In addition, 19 amplicon sequence variants were regulated according to the severity of the disease. Linear discriminant analysis effect size (LEfSe) also highlighted discriminant microbes in patients with fibrosis, such as an enrichment of Enterobacteriaceae and Escherichia/Shigella compared to patients with severe steatosis without fibrosis. All those data suggest a gut-liver-muscle axis in the pathogenesis of MAFLD complications.
format	article
author	Nicolas Lanthier Julie Rodriguez Maxime Nachit Sophie Hiel Pierre Trefois Audrey M. Neyrinck Patrice D. Cani Laure B. Bindels Jean-Paul Thissen Nathalie M. Delzenne
author_facet	Nicolas Lanthier Julie Rodriguez Maxime Nachit Sophie Hiel Pierre Trefois Audrey M. Neyrinck Patrice D. Cani Laure B. Bindels Jean-Paul Thissen Nathalie M. Delzenne
author_sort	Nicolas Lanthier
title	Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
title_short	Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
title_full	Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
title_fullStr	Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
title_full_unstemmed	Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
title_sort	microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/023ec02cb2dd45cc98da54f66da34aec
work_keys_str_mv	AT nicolaslanthier microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT julierodriguez microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT maximenachit microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT sophiehiel microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT pierretrefois microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT audreymneyrinck microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT patricedcani microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT laurebbindels microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT jeanpaulthissen microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients AT nathaliemdelzenne microbiotaanalysisandtransientelastographyrevealnewextrahepaticcomponentsofliversteatosisandfibrosisinobesepatients
_version_	1718387305653207040

Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients

Ejemplares similares