Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon

Abstract The macro- and micro-structures of mineralised tissues hierarchy are well described and understood. However, investigation of their nanostructure is limited due to the intrinsic complexity of biological systems. Preceding transmission electron microscopy studies investigating mineralising t...

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Autores principales:	Michał M. Kłosowski, Raffaella Carzaniga, Sandra J. Shefelbine, Alexandra E. Porter, David W. McComb
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0245ffc6919f469d8b5ee3bea8a4bbbd
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spelling	oai:doaj.org-article:0245ffc6919f469d8b5ee3bea8a4bbbd2021-12-02T15:08:41ZNanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon10.1038/s41598-018-20072-22045-2322https://doaj.org/article/0245ffc6919f469d8b5ee3bea8a4bbbd2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20072-2https://doaj.org/toc/2045-2322Abstract The macro- and micro-structures of mineralised tissues hierarchy are well described and understood. However, investigation of their nanostructure is limited due to the intrinsic complexity of biological systems. Preceding transmission electron microscopy studies investigating mineralising tissues have not resolved fully the initial stages of mineral nucleation and growth within the collagen fibrils. In this study, analytical scanning transmission electron microscopy and electron energy-loss spectroscopy were employed to characterise the morphology, crystallinity and chemistry of the mineral at different stages of mineralization using a turkey tendon model. In the poorly mineralised regions, calcium ions associated with the collagen fibrils and ellipsoidal granules and larger clusters composed of amorphous calcium phosphate were detected. In the fully mineralised regions, the mineral had transformed into crystalline apatite with a plate-like morphology. A change in the nitrogen K-edge was observed and related to modifications of the functional groups associated with the mineralisation process. This transformation seen in the nitrogen K-edge might be an important step in maturation and mineralisation of collagen and lend fundamental insight into how tendon mineralises.Michał M. KłosowskiRaffaella CarzanigaSandra J. ShefelbineAlexandra E. PorterDavid W. McCombNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michał M. Kłosowski Raffaella Carzaniga Sandra J. Shefelbine Alexandra E. Porter David W. McComb Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
description	Abstract The macro- and micro-structures of mineralised tissues hierarchy are well described and understood. However, investigation of their nanostructure is limited due to the intrinsic complexity of biological systems. Preceding transmission electron microscopy studies investigating mineralising tissues have not resolved fully the initial stages of mineral nucleation and growth within the collagen fibrils. In this study, analytical scanning transmission electron microscopy and electron energy-loss spectroscopy were employed to characterise the morphology, crystallinity and chemistry of the mineral at different stages of mineralization using a turkey tendon model. In the poorly mineralised regions, calcium ions associated with the collagen fibrils and ellipsoidal granules and larger clusters composed of amorphous calcium phosphate were detected. In the fully mineralised regions, the mineral had transformed into crystalline apatite with a plate-like morphology. A change in the nitrogen K-edge was observed and related to modifications of the functional groups associated with the mineralisation process. This transformation seen in the nitrogen K-edge might be an important step in maturation and mineralisation of collagen and lend fundamental insight into how tendon mineralises.
format	article
author	Michał M. Kłosowski Raffaella Carzaniga Sandra J. Shefelbine Alexandra E. Porter David W. McComb
author_facet	Michał M. Kłosowski Raffaella Carzaniga Sandra J. Shefelbine Alexandra E. Porter David W. McComb
author_sort	Michał M. Kłosowski
title	Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
title_short	Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
title_full	Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
title_fullStr	Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
title_full_unstemmed	Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
title_sort	nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0245ffc6919f469d8b5ee3bea8a4bbbd
work_keys_str_mv	AT michałmkłosowski nanoanalyticalelectronmicroscopyofeventspredisposingtomineralisationofturkeytendon AT raffaellacarzaniga nanoanalyticalelectronmicroscopyofeventspredisposingtomineralisationofturkeytendon AT sandrajshefelbine nanoanalyticalelectronmicroscopyofeventspredisposingtomineralisationofturkeytendon AT alexandraeporter nanoanalyticalelectronmicroscopyofeventspredisposingtomineralisationofturkeytendon AT davidwmccomb nanoanalyticalelectronmicroscopyofeventspredisposingtomineralisationofturkeytendon
_version_	1718388039610269696

Nanoanalytical electron microscopy of events predisposing to mineralisation of turkey tendon

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