Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator

Abstract A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5T, was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently...

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Autores principales:	Ahmed Saeedi, Nicola J. Cummings, Denise McLean, Ian F. Connerton, Phillippa L. Connerton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/024a314a8e654e06a450a870b7e3e18f
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spelling	oai:doaj.org-article:024a314a8e654e06a450a870b7e3e18f2021-11-08T10:52:15ZVenatorbacter cucullus gen. nov sp. nov a novel bacterial predator10.1038/s41598-021-00865-82045-2322https://doaj.org/article/024a314a8e654e06a450a870b7e3e18f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00865-8https://doaj.org/toc/2045-2322Abstract A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5T, was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found to serve as prey. Weak axenic growth on Brain Heart Infusion agar was achieved upon subculture without host cells. The optimal growth conditions were 37 °C, at pH 7. Transmission electron microscopy revealed some highly unusual morphological characteristics related to prey availability. Phylogenetic analyses using 16S rRNA gene sequences showed that the isolate was related to members of the Oceanospirillaceae family but could not be classified clearly as a member of any known genus. Whole genome sequencing of ASxL5T confirmed the relationship to members the Oceanospirillaceae. Database searches revealed that several ASxL5T share 16S rRNA gene sequences with several uncultured bacteria from marine, and terrestrial surface and subsurface water. We propose that strain ASxL5T represents a novel species in a new genus. We propose the name Venatorbacter cucullus gen. nov., sp. nov. with ASxL5T as the type strain.Ahmed SaeediNicola J. CummingsDenise McLeanIan F. ConnertonPhillippa L. ConnertonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmed Saeedi Nicola J. Cummings Denise McLean Ian F. Connerton Phillippa L. Connerton Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
description	Abstract A novel Gram-stain negative, aerobic, halotolerant, motile, rod-shaped, predatory bacterium ASxL5T, was isolated from a bovine slurry tank in Nottinghamshire, UK using Campylobacter hyointestinalis as prey. Other Campylobacter species and members of the Enterobacteriaceae were subsequently found to serve as prey. Weak axenic growth on Brain Heart Infusion agar was achieved upon subculture without host cells. The optimal growth conditions were 37 °C, at pH 7. Transmission electron microscopy revealed some highly unusual morphological characteristics related to prey availability. Phylogenetic analyses using 16S rRNA gene sequences showed that the isolate was related to members of the Oceanospirillaceae family but could not be classified clearly as a member of any known genus. Whole genome sequencing of ASxL5T confirmed the relationship to members the Oceanospirillaceae. Database searches revealed that several ASxL5T share 16S rRNA gene sequences with several uncultured bacteria from marine, and terrestrial surface and subsurface water. We propose that strain ASxL5T represents a novel species in a new genus. We propose the name Venatorbacter cucullus gen. nov., sp. nov. with ASxL5T as the type strain.
format	article
author	Ahmed Saeedi Nicola J. Cummings Denise McLean Ian F. Connerton Phillippa L. Connerton
author_facet	Ahmed Saeedi Nicola J. Cummings Denise McLean Ian F. Connerton Phillippa L. Connerton
author_sort	Ahmed Saeedi
title	Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
title_short	Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
title_full	Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
title_fullStr	Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
title_full_unstemmed	Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
title_sort	venatorbacter cucullus gen. nov sp. nov a novel bacterial predator
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/024a314a8e654e06a450a870b7e3e18f
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Venatorbacter cucullus gen. nov sp. nov a novel bacterial predator

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