The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface

Lu Sun,1 Guang Hong,2,3 Hiroyuki Matsui,1 Yun-Jia Song,1 Keiichi Sasaki1 1Division of Advanced Prosthetic Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 2Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku U...

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Autores principales:	Sun L, Hong G, Matsui H, Song YJ, Sasaki K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	syndecan zirconia cell adhesion osteoblastic cell Medicine (General) R5-920
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spelling	oai:doaj.org-article:025430a4dc364b89ada685c87e9f24092021-12-02T08:48:19ZThe Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface1178-2013https://doaj.org/article/025430a4dc364b89ada685c87e9f24092020-07-01T00:00:00Zhttps://www.dovepress.com/the-effects-of-syndecan-on-osteoblastic-cell-adhesion-onto-nano-zircon-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lu Sun,1 Guang Hong,2,3 Hiroyuki Matsui,1 Yun-Jia Song,1 Keiichi Sasaki1 1Division of Advanced Prosthetic Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 2Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 3Department of Prosthodontics, Faculty of Dental Medicine, Airlangga University, Surabaya, IndonesiaCorrespondence: Guang HongDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-Machi, Aoba-Ku, Sendai 980-8575, JapanTel +81-22-717-8259Email hong.guang.d6@tohoku.ac.jpPurpose: Zirconia is one of the most promising implant materials due to its favorable physical, mechanical and biological properties. However, until now, we know little about the mechanism of osseointegration on zirconia. The purpose of this study is to evaluate the effect of Syndecan (Sdc) on osteoblastic cell (MC3T3-E1) adhesion and proliferation onto zirconia materials.Materials and Methods: The mirror-polished disks 15 mm in diameter and 1.5 mm in thick of commercial pure titanium (CpTi), 3mol% yttria-stabilized tetragonal zirconia polycrystalline (3Y-TZP) and nano-zirconia (NanoZr) are used in this study. MC3T3-E1 cells were seeded onto specimen surfaces and subjected to RNA interference (RNAi) for Syndecan-1, Syndecan-2, Syndecan-3, and Syndecan-4. At 48h post-transfection, the cell morphology, actin cytoskeleton, and focal adhesion were observed using scanning electron microscopy or laser scanning confocal fluorescence microscopy. At 24h and 48h post-transfection, cell counting kit-8 (CCK-8) assay was used to investigate cell proliferation.Results: The cell morphology of MC3T3-E1 cells on CpTi, 3Y-TZP, and NanoZr changed into abnormal shape after gene silencing of Syndecan. Among the Syndecan family, Sdc-2 is responsible for NanoZr-specific morphology regulation, via maintenance of cytoskeletal conformation without affecting cellular attachment. According to CCK-8 assay, Sdc-2 affects the osteoblastic cell proliferation onto NanoZr.Conclusion: Within the limitation of this study, we suggest that Syndecan affects osteoblastic cell adhesion on CpTi, 3Y-TZP, and NanoZr. Sdc-2 might be an important heparin-sensitive cell membrane regulator in osteoblastic cell adhesion, specifically on NanoZr, through the organization of actin cytoskeleton and affects osteoblastic cell proliferation.Keywords: Syndecan, zirconia, cell adhesion, osteoblastic cellSun LHong GMatsui HSong YJSasaki KDove Medical Pressarticlesyndecanzirconiacell adhesionosteoblastic cellMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5061-5072 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	syndecan zirconia cell adhesion osteoblastic cell Medicine (General) R5-920
spellingShingle	syndecan zirconia cell adhesion osteoblastic cell Medicine (General) R5-920 Sun L Hong G Matsui H Song YJ Sasaki K The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface
description	Lu Sun,1 Guang Hong,2,3 Hiroyuki Matsui,1 Yun-Jia Song,1 Keiichi Sasaki1 1Division of Advanced Prosthetic Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 2Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 3Department of Prosthodontics, Faculty of Dental Medicine, Airlangga University, Surabaya, IndonesiaCorrespondence: Guang HongDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-Machi, Aoba-Ku, Sendai 980-8575, JapanTel +81-22-717-8259Email hong.guang.d6@tohoku.ac.jpPurpose: Zirconia is one of the most promising implant materials due to its favorable physical, mechanical and biological properties. However, until now, we know little about the mechanism of osseointegration on zirconia. The purpose of this study is to evaluate the effect of Syndecan (Sdc) on osteoblastic cell (MC3T3-E1) adhesion and proliferation onto zirconia materials.Materials and Methods: The mirror-polished disks 15 mm in diameter and 1.5 mm in thick of commercial pure titanium (CpTi), 3mol% yttria-stabilized tetragonal zirconia polycrystalline (3Y-TZP) and nano-zirconia (NanoZr) are used in this study. MC3T3-E1 cells were seeded onto specimen surfaces and subjected to RNA interference (RNAi) for Syndecan-1, Syndecan-2, Syndecan-3, and Syndecan-4. At 48h post-transfection, the cell morphology, actin cytoskeleton, and focal adhesion were observed using scanning electron microscopy or laser scanning confocal fluorescence microscopy. At 24h and 48h post-transfection, cell counting kit-8 (CCK-8) assay was used to investigate cell proliferation.Results: The cell morphology of MC3T3-E1 cells on CpTi, 3Y-TZP, and NanoZr changed into abnormal shape after gene silencing of Syndecan. Among the Syndecan family, Sdc-2 is responsible for NanoZr-specific morphology regulation, via maintenance of cytoskeletal conformation without affecting cellular attachment. According to CCK-8 assay, Sdc-2 affects the osteoblastic cell proliferation onto NanoZr.Conclusion: Within the limitation of this study, we suggest that Syndecan affects osteoblastic cell adhesion on CpTi, 3Y-TZP, and NanoZr. Sdc-2 might be an important heparin-sensitive cell membrane regulator in osteoblastic cell adhesion, specifically on NanoZr, through the organization of actin cytoskeleton and affects osteoblastic cell proliferation.Keywords: Syndecan, zirconia, cell adhesion, osteoblastic cell
format	article
author	Sun L Hong G Matsui H Song YJ Sasaki K
author_facet	Sun L Hong G Matsui H Song YJ Sasaki K
author_sort	Sun L
title	The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface
title_short	The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface
title_full	The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface
title_fullStr	The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface
title_full_unstemmed	The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface
title_sort	effects of syndecan on osteoblastic cell adhesion onto nano-zirconia surface
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/025430a4dc364b89ada685c87e9f2409
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The Effects of Syndecan on Osteoblastic Cell Adhesion Onto Nano-Zirconia Surface

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