Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation

Abstract The development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical c...

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Autores principales:	Rongkang Deng, Joseph P. Y. Kao, Patrick O. Kanold
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0258e50750d14b5f9393765fc78f8ed3
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spelling	oai:doaj.org-article:0258e50750d14b5f9393765fc78f8ed32021-12-02T14:06:31ZAberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation10.1038/s41598-021-82679-22045-2322https://doaj.org/article/0258e50750d14b5f9393765fc78f8ed32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82679-2https://doaj.org/toc/2045-2322Abstract The development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical circuits. These glutamatergic connections are mediated by glutamate receptors including AMPA and NMDA receptors and the ratio of AMPA to NMDA receptors decreases during development. Since previous studies have shown that retinal input can regulate the early development of connections along the visual pathway, we investigated if the maturation of glutamatergic inputs to GABAergic interneurons in the visual cortex requires retinal input. We mapped the spatial pattern of glutamatergic connections to layer 4 (L4) GABAergic interneurons in mouse visual cortex at around postnatal day (P) 16 by laser-scanning photostimulation and investigated the effect of binocular enucleations at P1/P2 on these patterns. Gad2-positive interneurons in enucleated animals showed an increased fraction of AMPAR-mediated input from L2/3 and a decreased fraction of input from L5/6. Parvalbumin-expressing (PV) interneurons showed similar changes in relative connectivity. NMDAR-only input was largely unchanged by enucleation. Our results show that retinal input sculpts the integration of interneurons into V1 circuits and suggest that the development of AMPAR- and NMDAR-only connections might be regulated differently.Rongkang DengJoseph P. Y. KaoPatrick O. KanoldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rongkang Deng Joseph P. Y. Kao Patrick O. Kanold Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
description	Abstract The development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical circuits. These glutamatergic connections are mediated by glutamate receptors including AMPA and NMDA receptors and the ratio of AMPA to NMDA receptors decreases during development. Since previous studies have shown that retinal input can regulate the early development of connections along the visual pathway, we investigated if the maturation of glutamatergic inputs to GABAergic interneurons in the visual cortex requires retinal input. We mapped the spatial pattern of glutamatergic connections to layer 4 (L4) GABAergic interneurons in mouse visual cortex at around postnatal day (P) 16 by laser-scanning photostimulation and investigated the effect of binocular enucleations at P1/P2 on these patterns. Gad2-positive interneurons in enucleated animals showed an increased fraction of AMPAR-mediated input from L2/3 and a decreased fraction of input from L5/6. Parvalbumin-expressing (PV) interneurons showed similar changes in relative connectivity. NMDAR-only input was largely unchanged by enucleation. Our results show that retinal input sculpts the integration of interneurons into V1 circuits and suggest that the development of AMPAR- and NMDAR-only connections might be regulated differently.
format	article
author	Rongkang Deng Joseph P. Y. Kao Patrick O. Kanold
author_facet	Rongkang Deng Joseph P. Y. Kao Patrick O. Kanold
author_sort	Rongkang Deng
title	Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
title_short	Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
title_full	Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
title_fullStr	Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
title_full_unstemmed	Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
title_sort	aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0258e50750d14b5f9393765fc78f8ed3
work_keys_str_mv	AT rongkangdeng aberrantdevelopmentofexcitatorycircuitstoinhibitoryneuronsintheprimaryvisualcortexafterneonatalbinocularenucleation AT josephpykao aberrantdevelopmentofexcitatorycircuitstoinhibitoryneuronsintheprimaryvisualcortexafterneonatalbinocularenucleation AT patrickokanold aberrantdevelopmentofexcitatorycircuitstoinhibitoryneuronsintheprimaryvisualcortexafterneonatalbinocularenucleation
_version_	1718391942827474944

Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation

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