Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics

ABSTRACT Physical contact between yeast species, in addition to better-understood and reported metabolic interactions, has recently been proposed to significantly impact the relative fitness of these species in cocultures. Such data have been generated by using membrane bioreactors, which physically...

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Autores principales:	Debra Rossouw, Skye P. Meiring, Florian F. Bauer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	adhesion cell-cell interaction interspecies yeast Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0264e56e12a34838900dbc872d685a9d
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spelling	oai:doaj.org-article:0264e56e12a34838900dbc872d685a9d2021-11-15T15:22:26ZModifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics10.1128/mSphere.00383-182379-5042https://doaj.org/article/0264e56e12a34838900dbc872d685a9d2018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00383-18https://doaj.org/toc/2379-5042ABSTRACT Physical contact between yeast species, in addition to better-understood and reported metabolic interactions, has recently been proposed to significantly impact the relative fitness of these species in cocultures. Such data have been generated by using membrane bioreactors, which physically separate two yeast species. However, doubts persist about the degree that the various membrane systems allow for continuous and complete metabolic contact, including the exchange of proteins. Here, we provide independent evidence for the importance of physical contact by using a genetic system to modify the degree of physical contact and, therefore, the degree of asexual intraspecies and interspecies adhesion in yeast. Such adhesion is controlled by a family of structurally related cell wall proteins encoded by the FLO gene family. As previously shown, the expression of specific members of the FLO gene family in Saccharomyces cerevisiae dramatically changes the coadhesion patterns between this yeast and other yeast species. Here, we use this differential aggregation mediated by FLO genes as a model to assess the impact of physical contact between different yeast species on the relative fitness of these species in simplified ecosystems. The identity of the FLO gene has a marked effect on the persistence of specific non-Saccharomyces yeasts over the course of extended growth periods in batch cultures. Remarkably, FLO1 and FLO5 expression often result in opposite outcomes. The data provide clear evidence for the role of physical contact in multispecies yeast ecosystems and suggest that FLO gene expression may be a major factor in such interactions. IMPORTANCE The impact of direct (physical) versus indirect (metabolic) interactions between different yeast species has attracted significant research interest in recent years. This is due to the growing interest in the use of multispecies consortia in bioprocesses of industrial relevance and the relevance of interspecies interactions in establishing stable synthetic ecosystems. Compartment bioreactors have traditionally been used in this regard but suffer from numerous limitations. Here, we provide independent evidence for the importance of physical contact by using a genetic system, based on the FLO gene family, to modify the degree of physical contact and, therefore, the degree of asexual intraspecies and interspecies adhesion in yeast. Our results show that interspecies contact significantly impacts population dynamics and the survival of individual species. Remarkably, different members of the FLO gene family often lead to very different population outcomes, further suggesting that FLO gene expression may be a major factor in such interactions.Debra RossouwSkye P. MeiringFlorian F. BauerAmerican Society for Microbiologyarticleadhesioncell-cell interactioninterspeciesyeastMicrobiologyQR1-502ENmSphere, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	adhesion cell-cell interaction interspecies yeast Microbiology QR1-502
spellingShingle	adhesion cell-cell interaction interspecies yeast Microbiology QR1-502 Debra Rossouw Skye P. Meiring Florian F. Bauer Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics
description	ABSTRACT Physical contact between yeast species, in addition to better-understood and reported metabolic interactions, has recently been proposed to significantly impact the relative fitness of these species in cocultures. Such data have been generated by using membrane bioreactors, which physically separate two yeast species. However, doubts persist about the degree that the various membrane systems allow for continuous and complete metabolic contact, including the exchange of proteins. Here, we provide independent evidence for the importance of physical contact by using a genetic system to modify the degree of physical contact and, therefore, the degree of asexual intraspecies and interspecies adhesion in yeast. Such adhesion is controlled by a family of structurally related cell wall proteins encoded by the FLO gene family. As previously shown, the expression of specific members of the FLO gene family in Saccharomyces cerevisiae dramatically changes the coadhesion patterns between this yeast and other yeast species. Here, we use this differential aggregation mediated by FLO genes as a model to assess the impact of physical contact between different yeast species on the relative fitness of these species in simplified ecosystems. The identity of the FLO gene has a marked effect on the persistence of specific non-Saccharomyces yeasts over the course of extended growth periods in batch cultures. Remarkably, FLO1 and FLO5 expression often result in opposite outcomes. The data provide clear evidence for the role of physical contact in multispecies yeast ecosystems and suggest that FLO gene expression may be a major factor in such interactions. IMPORTANCE The impact of direct (physical) versus indirect (metabolic) interactions between different yeast species has attracted significant research interest in recent years. This is due to the growing interest in the use of multispecies consortia in bioprocesses of industrial relevance and the relevance of interspecies interactions in establishing stable synthetic ecosystems. Compartment bioreactors have traditionally been used in this regard but suffer from numerous limitations. Here, we provide independent evidence for the importance of physical contact by using a genetic system, based on the FLO gene family, to modify the degree of physical contact and, therefore, the degree of asexual intraspecies and interspecies adhesion in yeast. Our results show that interspecies contact significantly impacts population dynamics and the survival of individual species. Remarkably, different members of the FLO gene family often lead to very different population outcomes, further suggesting that FLO gene expression may be a major factor in such interactions.
format	article
author	Debra Rossouw Skye P. Meiring Florian F. Bauer
author_facet	Debra Rossouw Skye P. Meiring Florian F. Bauer
author_sort	Debra Rossouw
title	Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics
title_short	Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics
title_full	Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics
title_fullStr	Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics
title_full_unstemmed	Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics
title_sort	modifying <named-content content-type="genus-species">saccharomyces cerevisiae</named-content> adhesion properties regulates yeast ecosystem dynamics
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/0264e56e12a34838900dbc872d685a9d
work_keys_str_mv	AT debrarossouw modifyingnamedcontentcontenttypegenusspeciessaccharomycescerevisiaenamedcontentadhesionpropertiesregulatesyeastecosystemdynamics AT skyepmeiring modifyingnamedcontentcontenttypegenusspeciessaccharomycescerevisiaenamedcontentadhesionpropertiesregulatesyeastecosystemdynamics AT florianfbauer modifyingnamedcontentcontenttypegenusspeciessaccharomycescerevisiaenamedcontentadhesionpropertiesregulatesyeastecosystemdynamics
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Modifying <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content> Adhesion Properties Regulates Yeast Ecosystem Dynamics

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