The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.

Each year, during winter months, human Metapneumovirus (hMPV) is associated with epidemics of bronchiolitis resulting in the hospitalization of many infants. Bronchiolitis is an acute illness of the lower respiratory tract with a consequent inflammation of the bronchioles. The rapid onset of inflamm...

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Autores principales:	Audrey Bagnaud-Baule, Olivier Reynard, Magali Perret, Jean-Luc Berland, Mimoun Maache, Christophe Peyrefitte, Guy Vernet, Viktor Volchkov, Gláucia Paranhos-Baccalà
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/026ae76158eb4697a37179a9931f4919
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spelling	oai:doaj.org-article:026ae76158eb4697a37179a9931f49192021-11-18T06:57:23ZThe human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.1932-620310.1371/journal.pone.0017818https://doaj.org/article/026ae76158eb4697a37179a9931f49192011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21412439/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Each year, during winter months, human Metapneumovirus (hMPV) is associated with epidemics of bronchiolitis resulting in the hospitalization of many infants. Bronchiolitis is an acute illness of the lower respiratory tract with a consequent inflammation of the bronchioles. The rapid onset of inflammation suggests the innate immune response may have a role to play in the pathogenesis of this hMPV infection. Since, the matrix protein is one of the most abundant proteins in the Paramyxoviridae family virion, we hypothesized that the inflammatory modulation observed in hMPV infected patients may be partly associated with the matrix protein (M-hMPV) response. By western blot analysis, we detected a soluble form of M-hMPV released from hMPV infected cell as well as from M-hMPV transfected HEK 293T cells suggesting that M-hMPV may be directly in contact with antigen presenting cells (APCs) during the course of infection. Moreover, flow cytometry and confocal microscopy allowed determining that M-hMPV was taken up by dendritic cells (moDCs) and macrophages inducing their activation. Furthermore, these moDCs enter into a maturation process inducing the secretion of a broad range of inflammatory cytokines when exposed to M-hMPV. Additionally, M-hMPV activated DCs were shown to stimulate IL-2 and IFN-γ production by allogeneic T lymphocytes. This M-hMPV-mediated activation and antigen presentation of APCs may in part explain the marked inflammatory immune response observed in pathology induced by hMPV in patients.Audrey Bagnaud-BauleOlivier ReynardMagali PerretJean-Luc BerlandMimoun MaacheChristophe PeyrefitteGuy VernetViktor VolchkovGláucia Paranhos-BaccalàPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17818 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Audrey Bagnaud-Baule Olivier Reynard Magali Perret Jean-Luc Berland Mimoun Maache Christophe Peyrefitte Guy Vernet Viktor Volchkov Gláucia Paranhos-Baccalà The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
description	Each year, during winter months, human Metapneumovirus (hMPV) is associated with epidemics of bronchiolitis resulting in the hospitalization of many infants. Bronchiolitis is an acute illness of the lower respiratory tract with a consequent inflammation of the bronchioles. The rapid onset of inflammation suggests the innate immune response may have a role to play in the pathogenesis of this hMPV infection. Since, the matrix protein is one of the most abundant proteins in the Paramyxoviridae family virion, we hypothesized that the inflammatory modulation observed in hMPV infected patients may be partly associated with the matrix protein (M-hMPV) response. By western blot analysis, we detected a soluble form of M-hMPV released from hMPV infected cell as well as from M-hMPV transfected HEK 293T cells suggesting that M-hMPV may be directly in contact with antigen presenting cells (APCs) during the course of infection. Moreover, flow cytometry and confocal microscopy allowed determining that M-hMPV was taken up by dendritic cells (moDCs) and macrophages inducing their activation. Furthermore, these moDCs enter into a maturation process inducing the secretion of a broad range of inflammatory cytokines when exposed to M-hMPV. Additionally, M-hMPV activated DCs were shown to stimulate IL-2 and IFN-γ production by allogeneic T lymphocytes. This M-hMPV-mediated activation and antigen presentation of APCs may in part explain the marked inflammatory immune response observed in pathology induced by hMPV in patients.
format	article
author	Audrey Bagnaud-Baule Olivier Reynard Magali Perret Jean-Luc Berland Mimoun Maache Christophe Peyrefitte Guy Vernet Viktor Volchkov Gláucia Paranhos-Baccalà
author_facet	Audrey Bagnaud-Baule Olivier Reynard Magali Perret Jean-Luc Berland Mimoun Maache Christophe Peyrefitte Guy Vernet Viktor Volchkov Gláucia Paranhos-Baccalà
author_sort	Audrey Bagnaud-Baule
title	The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
title_short	The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
title_full	The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
title_fullStr	The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
title_full_unstemmed	The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
title_sort	human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/026ae76158eb4697a37179a9931f4919
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The human metapneumovirus matrix protein stimulates the inflammatory immune response in vitro.

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