Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy

Two-photon microscopy is a powerful tool for studying neuronal activity but cannot easily image deeper cortical layers. Here, the authors design a custom microscope for three-photon microscopy and use it to reveal response properties of layer 5, 6, and subplate visual cortical neurons.

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Bibliographic Details
Main Authors: Murat Yildirim, Hiroki Sugihara, Peter T. C. So, Mriganka Sur
Format: article
Language:EN
Published: Nature Portfolio 2019
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Online Access:https://doaj.org/article/026c5287de584c509e5a5115ae25ff45
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