Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood

In the last few years, liquid chromatography coupled with mass spectrometry (LC/MS) has been increasingly used for screening purposes in forensic toxicology. These techniques have the advantages of low time/resource-consuming and high versatility and have been applied in numerous new multi-analytes...

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Autores principales:	Fabio Vaiano, Elisabetta Bertol, Maria Mineo, Laura Pietrosemoli, Jolanda Rubicondo, Claudiu T. Supuran, Fabrizio Carta
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	new psychoactive substances LC-MS/MS blood benzodiazepine drugs of abuse screening Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/026c82ee21204cbdbbad5cf33e2c597b
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spelling	oai:doaj.org-article:026c82ee21204cbdbbad5cf33e2c597b2021-11-25T18:59:28ZDevelopment of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood10.3390/separations81102212297-8739https://doaj.org/article/026c82ee21204cbdbbad5cf33e2c597b2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/221https://doaj.org/toc/2297-8739In the last few years, liquid chromatography coupled with mass spectrometry (LC/MS) has been increasingly used for screening purposes in forensic toxicology. These techniques have the advantages of low time/resource-consuming and high versatility and have been applied in numerous new multi-analytes methods. The new psychoactive substance (NPS) phenomenon provided a great impulse to this wide-range approach, but it is also important to keep the attention on “classical” psychoactive substances, such as benzodiazepines (BDZ). In this paper, a fully validated screening method in blood for the simultaneous detection of 163 substances (120 NPS and 43 other drugs) by a dynamic multiple reaction monitoring analysis through LC-MS/MS is described. The method consists of a deproteinization of 200 µL of blood with acetonitrile. The LC separation is achieved with a 100 mm long C18 column in 35 min. The method was very sensitive, with limits of quantification from 0.02 to 1.5 ng/mL. Matrix effects did not negatively affect the analytical sensitivity. This method proved to be reliable and was successfully applied to our routinary analytical activity in several forensic caseworks, allowing the identification and quantification of many BDZs and 3,4-methylenedioxypyrovalerone (MDPV).Fabio VaianoElisabetta BertolMaria MineoLaura PietrosemoliJolanda RubicondoClaudiu T. SupuranFabrizio CartaMDPI AGarticlenew psychoactive substancesLC-MS/MSbloodbenzodiazepinedrugs of abusescreeningPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 221, p 221 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	new psychoactive substances LC-MS/MS blood benzodiazepine drugs of abuse screening Physics QC1-999 Chemistry QD1-999
spellingShingle	new psychoactive substances LC-MS/MS blood benzodiazepine drugs of abuse screening Physics QC1-999 Chemistry QD1-999 Fabio Vaiano Elisabetta Bertol Maria Mineo Laura Pietrosemoli Jolanda Rubicondo Claudiu T. Supuran Fabrizio Carta Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood
description	In the last few years, liquid chromatography coupled with mass spectrometry (LC/MS) has been increasingly used for screening purposes in forensic toxicology. These techniques have the advantages of low time/resource-consuming and high versatility and have been applied in numerous new multi-analytes methods. The new psychoactive substance (NPS) phenomenon provided a great impulse to this wide-range approach, but it is also important to keep the attention on “classical” psychoactive substances, such as benzodiazepines (BDZ). In this paper, a fully validated screening method in blood for the simultaneous detection of 163 substances (120 NPS and 43 other drugs) by a dynamic multiple reaction monitoring analysis through LC-MS/MS is described. The method consists of a deproteinization of 200 µL of blood with acetonitrile. The LC separation is achieved with a 100 mm long C18 column in 35 min. The method was very sensitive, with limits of quantification from 0.02 to 1.5 ng/mL. Matrix effects did not negatively affect the analytical sensitivity. This method proved to be reliable and was successfully applied to our routinary analytical activity in several forensic caseworks, allowing the identification and quantification of many BDZs and 3,4-methylenedioxypyrovalerone (MDPV).
format	article
author	Fabio Vaiano Elisabetta Bertol Maria Mineo Laura Pietrosemoli Jolanda Rubicondo Claudiu T. Supuran Fabrizio Carta
author_facet	Fabio Vaiano Elisabetta Bertol Maria Mineo Laura Pietrosemoli Jolanda Rubicondo Claudiu T. Supuran Fabrizio Carta
author_sort	Fabio Vaiano
title	Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood
title_short	Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood
title_full	Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood
title_fullStr	Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood
title_full_unstemmed	Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood
title_sort	development of a new lc-ms/ms screening method for detection of 120 nps and 43 drugs in blood
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/026c82ee21204cbdbbad5cf33e2c597b
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Development of a New LC-MS/MS Screening Method for Detection of 120 NPS and 43 Drugs in Blood

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