HSPB1 facilitates the formation of non-centrosomal microtubules.

The remodeling capacity of microtubules (MT) is essential for their proper function. In mammals, MTs are predominantly formed at the centrosome, but can also originate from non-centrosomal sites, a process that is still poorly understood. We here show that the small heat shock protein HSPB1 plays a...

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Autores principales:	Leonardo Almeida-Souza, Bob Asselbergh, Vicky De Winter, Sofie Goethals, Vincent Timmerman, Sophie Janssens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02771c3c5cdf4a4b8354925e345fde5c
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id	oai:doaj.org-article:02771c3c5cdf4a4b8354925e345fde5c
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spelling	oai:doaj.org-article:02771c3c5cdf4a4b8354925e345fde5c2021-11-18T07:40:28ZHSPB1 facilitates the formation of non-centrosomal microtubules.1932-620310.1371/journal.pone.0066541https://doaj.org/article/02771c3c5cdf4a4b8354925e345fde5c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826100/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The remodeling capacity of microtubules (MT) is essential for their proper function. In mammals, MTs are predominantly formed at the centrosome, but can also originate from non-centrosomal sites, a process that is still poorly understood. We here show that the small heat shock protein HSPB1 plays a role in the control of non-centrosomal MT formation. The HSPB1 expression level regulates the balance between centrosomal and non-centrosomal MTs. The HSPB1 protein can be detected specifically at sites of de novo forming non-centrosomal MTs, while it is absent from the centrosomes. In addition, we show that HSPB1 binds preferentially to the lattice of newly formed MTs in vitro, suggesting that its function occurs by stabilizing MT seeds. Our findings open new avenues for the understanding of the role of HSPB1 in the development, maintenance and protection of cells with specialized non-centrosomal MT arrays.Leonardo Almeida-SouzaBob AsselberghVicky De WinterSofie GoethalsVincent TimmermanSophie JanssensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66541 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leonardo Almeida-Souza Bob Asselbergh Vicky De Winter Sofie Goethals Vincent Timmerman Sophie Janssens HSPB1 facilitates the formation of non-centrosomal microtubules.
description	The remodeling capacity of microtubules (MT) is essential for their proper function. In mammals, MTs are predominantly formed at the centrosome, but can also originate from non-centrosomal sites, a process that is still poorly understood. We here show that the small heat shock protein HSPB1 plays a role in the control of non-centrosomal MT formation. The HSPB1 expression level regulates the balance between centrosomal and non-centrosomal MTs. The HSPB1 protein can be detected specifically at sites of de novo forming non-centrosomal MTs, while it is absent from the centrosomes. In addition, we show that HSPB1 binds preferentially to the lattice of newly formed MTs in vitro, suggesting that its function occurs by stabilizing MT seeds. Our findings open new avenues for the understanding of the role of HSPB1 in the development, maintenance and protection of cells with specialized non-centrosomal MT arrays.
format	article
author	Leonardo Almeida-Souza Bob Asselbergh Vicky De Winter Sofie Goethals Vincent Timmerman Sophie Janssens
author_facet	Leonardo Almeida-Souza Bob Asselbergh Vicky De Winter Sofie Goethals Vincent Timmerman Sophie Janssens
author_sort	Leonardo Almeida-Souza
title	HSPB1 facilitates the formation of non-centrosomal microtubules.
title_short	HSPB1 facilitates the formation of non-centrosomal microtubules.
title_full	HSPB1 facilitates the formation of non-centrosomal microtubules.
title_fullStr	HSPB1 facilitates the formation of non-centrosomal microtubules.
title_full_unstemmed	HSPB1 facilitates the formation of non-centrosomal microtubules.
title_sort	hspb1 facilitates the formation of non-centrosomal microtubules.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/02771c3c5cdf4a4b8354925e345fde5c
work_keys_str_mv	AT leonardoalmeidasouza hspb1facilitatestheformationofnoncentrosomalmicrotubules AT bobasselbergh hspb1facilitatestheformationofnoncentrosomalmicrotubules AT vickydewinter hspb1facilitatestheformationofnoncentrosomalmicrotubules AT sofiegoethals hspb1facilitatestheformationofnoncentrosomalmicrotubules AT vincenttimmerman hspb1facilitatestheformationofnoncentrosomalmicrotubules AT sophiejanssens hspb1facilitatestheformationofnoncentrosomalmicrotubules
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HSPB1 facilitates the formation of non-centrosomal microtubules.

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