Protein encapsulation in polymeric microneedles by photolithography

Jaspreet Singh Kochhar,1 Shui Zou,2 Sui Yung Chan,1 Lifeng Kang11Department of Pharmacy, 2Department of Chemistry, National University of Singapore, SingaporeBackground: Recent interest in biocompatible polymeric microneedles for the delivery of biomolecules has propelled considerable interest in fa...

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Autores principales:	Kochhar JS, Zou S, Chan SY, Kang L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/028108cb3a89465b89d831be90b43c47
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spelling	oai:doaj.org-article:028108cb3a89465b89d831be90b43c472021-12-02T02:13:41ZProtein encapsulation in polymeric microneedles by photolithography1176-91141178-2013https://doaj.org/article/028108cb3a89465b89d831be90b43c472012-06-01T00:00:00Zhttp://www.dovepress.com/protein-encapsulation-in-polymeric-microneedles-by-photolithography-a10198https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jaspreet Singh Kochhar,1 Shui Zou,2 Sui Yung Chan,1 Lifeng Kang11Department of Pharmacy, 2Department of Chemistry, National University of Singapore, SingaporeBackground: Recent interest in biocompatible polymeric microneedles for the delivery of biomolecules has propelled considerable interest in fabrication of microneedles. It is important that the fabrication process is feasible for drug encapsulation and compatible with the stability of the drug in question. Moreover, drug encapsulation may offer the advantage of higher drug loading compared with other technologies, such as drug coating.Methods and results: In this study, we encapsulated a model protein drug, namely, bovine serum albumin, in polymeric microneedles by photolithography. Drug distribution within the microneedle array was found to be uniform. The encapsulated protein retained its primary, secondary, and tertiary structural characteristics. In vitro release of the encapsulated protein showed that almost all of the drug was released into phosphate buffered saline within 6 hours. The in vitro permeation profile of encapsulated bovine serum albumin through rat skin was also tested and shown to resemble the in vitro release profile, with an initial release burst followed by a slow release phase. The cytotoxicity of the microneedles without bovine serum albumin was tested in three different cell lines. High cell viabilities were observed, demonstrating the innocuous nature of the microneedles.Conclusion: The microneedle array can potentially serve as a useful drug carrier for proteins, peptides, and vaccines.Keywords: poly (ethylene glycol) diacrylate, microneedles, protein stability, photolithography, biocompatibilityKochhar JSZou SChan SYKang LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3143-3154 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kochhar JS Zou S Chan SY Kang L Protein encapsulation in polymeric microneedles by photolithography
description	Jaspreet Singh Kochhar,1 Shui Zou,2 Sui Yung Chan,1 Lifeng Kang11Department of Pharmacy, 2Department of Chemistry, National University of Singapore, SingaporeBackground: Recent interest in biocompatible polymeric microneedles for the delivery of biomolecules has propelled considerable interest in fabrication of microneedles. It is important that the fabrication process is feasible for drug encapsulation and compatible with the stability of the drug in question. Moreover, drug encapsulation may offer the advantage of higher drug loading compared with other technologies, such as drug coating.Methods and results: In this study, we encapsulated a model protein drug, namely, bovine serum albumin, in polymeric microneedles by photolithography. Drug distribution within the microneedle array was found to be uniform. The encapsulated protein retained its primary, secondary, and tertiary structural characteristics. In vitro release of the encapsulated protein showed that almost all of the drug was released into phosphate buffered saline within 6 hours. The in vitro permeation profile of encapsulated bovine serum albumin through rat skin was also tested and shown to resemble the in vitro release profile, with an initial release burst followed by a slow release phase. The cytotoxicity of the microneedles without bovine serum albumin was tested in three different cell lines. High cell viabilities were observed, demonstrating the innocuous nature of the microneedles.Conclusion: The microneedle array can potentially serve as a useful drug carrier for proteins, peptides, and vaccines.Keywords: poly (ethylene glycol) diacrylate, microneedles, protein stability, photolithography, biocompatibility
format	article
author	Kochhar JS Zou S Chan SY Kang L
author_facet	Kochhar JS Zou S Chan SY Kang L
author_sort	Kochhar JS
title	Protein encapsulation in polymeric microneedles by photolithography
title_short	Protein encapsulation in polymeric microneedles by photolithography
title_full	Protein encapsulation in polymeric microneedles by photolithography
title_fullStr	Protein encapsulation in polymeric microneedles by photolithography
title_full_unstemmed	Protein encapsulation in polymeric microneedles by photolithography
title_sort	protein encapsulation in polymeric microneedles by photolithography
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/028108cb3a89465b89d831be90b43c47
work_keys_str_mv	AT kochharjs proteinencapsulationinpolymericmicroneedlesbyphotolithography AT zous proteinencapsulationinpolymericmicroneedlesbyphotolithography AT chansy proteinencapsulationinpolymericmicroneedlesbyphotolithography AT kangl proteinencapsulationinpolymericmicroneedlesbyphotolithography
_version_	1718402569503506432

Protein encapsulation in polymeric microneedles by photolithography

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