Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis

Abstract Lowering cost will prompt the sustainable development of sugarcane‐based ethanol industry. In this work, we developed a low‐cost process for ethanol production from sugarcane by a genetically engineered Zymomonas mobilis. Fermentation media were first optimized, resulting in a 15.54% increa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yonghua Dai, Bo Wu, Panting Liu, Mao Chen, Chao Song, Qiyu Gou, Renbin Liu, Yansheng Xu, Guoquan Hu, Mingxiong He
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	bioethanol CRISPR‐Cas fed‐batch fermentation nitrogen source sugarcane juice Zymomonas mobilis Renewable energy sources TJ807-830 Energy industries. Energy policy. Fuel trade HD9502-9502.5
Acceso en línea:	https://doaj.org/article/0281517d8e754cf3b91050a0fc571baf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0281517d8e754cf3b91050a0fc571baf
record_format	dspace
spelling	oai:doaj.org-article:0281517d8e754cf3b91050a0fc571baf2021-11-09T02:26:56ZLowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis1757-17071757-169310.1111/gcbb.12891https://doaj.org/article/0281517d8e754cf3b91050a0fc571baf2021-12-01T00:00:00Zhttps://doi.org/10.1111/gcbb.12891https://doaj.org/toc/1757-1693https://doaj.org/toc/1757-1707Abstract Lowering cost will prompt the sustainable development of sugarcane‐based ethanol industry. In this work, we developed a low‐cost process for ethanol production from sugarcane by a genetically engineered Zymomonas mobilis. Fermentation media were first optimized, resulting in a 15.54% increase in ethanol fermentation efficiency as compared to control media. To further reduce the byproduct levan formation, a levansucrase‐encoding gene of Z. mobilis, sacB, was deleted through the type I‐F CRISPR‐Cas system, which resulted in a further elevation of both ethanol conversion ratio and productivity comparing with the starting strain ZMS912 (87.50% vs. 76.77%, 1.95 g/L/h vs. 1.71 g/L/h). Moreover, we conducted fed‐batch fermentation for ethanol production using sugarcane juice in 5 L bioreactors and employing the optimized media and engineered strain. The results showed that maximum ethanol titer of 81.59 g/L and productivity of 5.83 g/L/h were achieved. Finally, preliminary techno‐economic assessment demonstrated that our efforts to modify media and strain could reduce the processing cost of ethanol production from sugarcane juice, which provides the feasibility for economic ethanol production in the future.Yonghua DaiBo WuPanting LiuMao ChenChao SongQiyu GouRenbin LiuYansheng XuGuoquan HuMingxiong HeWileyarticlebioethanolCRISPR‐Casfed‐batch fermentationnitrogen sourcesugarcane juiceZymomonas mobilisRenewable energy sourcesTJ807-830Energy industries. Energy policy. Fuel tradeHD9502-9502.5ENGCB Bioenergy, Vol 13, Iss 12, Pp 1894-1907 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioethanol CRISPR‐Cas fed‐batch fermentation nitrogen source sugarcane juice Zymomonas mobilis Renewable energy sources TJ807-830 Energy industries. Energy policy. Fuel trade HD9502-9502.5
spellingShingle	bioethanol CRISPR‐Cas fed‐batch fermentation nitrogen source sugarcane juice Zymomonas mobilis Renewable energy sources TJ807-830 Energy industries. Energy policy. Fuel trade HD9502-9502.5 Yonghua Dai Bo Wu Panting Liu Mao Chen Chao Song Qiyu Gou Renbin Liu Yansheng Xu Guoquan Hu Mingxiong He Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis
description	Abstract Lowering cost will prompt the sustainable development of sugarcane‐based ethanol industry. In this work, we developed a low‐cost process for ethanol production from sugarcane by a genetically engineered Zymomonas mobilis. Fermentation media were first optimized, resulting in a 15.54% increase in ethanol fermentation efficiency as compared to control media. To further reduce the byproduct levan formation, a levansucrase‐encoding gene of Z. mobilis, sacB, was deleted through the type I‐F CRISPR‐Cas system, which resulted in a further elevation of both ethanol conversion ratio and productivity comparing with the starting strain ZMS912 (87.50% vs. 76.77%, 1.95 g/L/h vs. 1.71 g/L/h). Moreover, we conducted fed‐batch fermentation for ethanol production using sugarcane juice in 5 L bioreactors and employing the optimized media and engineered strain. The results showed that maximum ethanol titer of 81.59 g/L and productivity of 5.83 g/L/h were achieved. Finally, preliminary techno‐economic assessment demonstrated that our efforts to modify media and strain could reduce the processing cost of ethanol production from sugarcane juice, which provides the feasibility for economic ethanol production in the future.
format	article
author	Yonghua Dai Bo Wu Panting Liu Mao Chen Chao Song Qiyu Gou Renbin Liu Yansheng Xu Guoquan Hu Mingxiong He
author_facet	Yonghua Dai Bo Wu Panting Liu Mao Chen Chao Song Qiyu Gou Renbin Liu Yansheng Xu Guoquan Hu Mingxiong He
author_sort	Yonghua Dai
title	Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis
title_short	Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis
title_full	Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis
title_fullStr	Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis
title_full_unstemmed	Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis
title_sort	lowering whole cost for sugarcane‐based ethanol production by engineered zymomonas mobilis
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/0281517d8e754cf3b91050a0fc571baf
work_keys_str_mv	AT yonghuadai loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT bowu loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT pantingliu loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT maochen loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT chaosong loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT qiyugou loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT renbinliu loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT yanshengxu loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT guoquanhu loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis AT mingxionghe loweringwholecostforsugarcanebasedethanolproductionbyengineeredzymomonasmobilis
_version_	1718441354876420096

Lowering whole cost for sugarcane‐based ethanol production by engineered Zymomonas mobilis

Ejemplares similares