Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice

Abstract We investigated and compared the susceptibility of retinal blood flow regulation and neural function in mice developing type 2 diabetes. The longitudinal changes in retinal neuronal function and blood flow responses to a 10-min systemic hyperoxia and a 3-min flicker stimulation were evaluat...

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Autores principales:	Junya Hanaguri, Harumasa Yokota, Masahisa Watanabe, Satoru Yamagami, Akifumi Kushiyama, Lih Kuo, Taiji Nagaoka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02862ec994d94060ad2eb7b8803bd4e0
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spelling	oai:doaj.org-article:02862ec994d94060ad2eb7b8803bd4e02021-12-02T18:02:23ZRetinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice10.1038/s41598-021-97651-32045-2322https://doaj.org/article/02862ec994d94060ad2eb7b8803bd4e02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97651-3https://doaj.org/toc/2045-2322Abstract We investigated and compared the susceptibility of retinal blood flow regulation and neural function in mice developing type 2 diabetes. The longitudinal changes in retinal neuronal function and blood flow responses to a 10-min systemic hyperoxia and a 3-min flicker stimulation were evaluated every 2 weeks in diabetic db/db mice and nondiabetic controls (db/m) from age 8 to 20 weeks. The retinal blood flow and neural activity were assessed using laser speckle flowgraphy and electroretinography (ERG), respectively. The db/db mice had significantly higher blood glucose levels and body weight. The resting retinal blood flow was steady and comparable between two groups throughout the study. Hyperoxia elicited a consistent decrease, and flicker light an increase, in retinal blood flow in db/m mice independent of age. However, these flow responses were significantly diminished in db/db mice at 8 weeks old and then the mice became unresponsive to stimulations at 12 weeks. Subsequently, the ERG implicit time for oscillatory potential was significantly increased at 14 weeks of age while the a-wave and b-wave amplitudes and implicit times remained unchanged. The deficiencies of flow regulation and neurovascular coupling in the retina appear to precede neural dysfunction in the mouse with type 2 diabetes.Junya HanaguriHarumasa YokotaMasahisa WatanabeSatoru YamagamiAkifumi KushiyamaLih KuoTaiji NagaokaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Junya Hanaguri Harumasa Yokota Masahisa Watanabe Satoru Yamagami Akifumi Kushiyama Lih Kuo Taiji Nagaoka Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
description	Abstract We investigated and compared the susceptibility of retinal blood flow regulation and neural function in mice developing type 2 diabetes. The longitudinal changes in retinal neuronal function and blood flow responses to a 10-min systemic hyperoxia and a 3-min flicker stimulation were evaluated every 2 weeks in diabetic db/db mice and nondiabetic controls (db/m) from age 8 to 20 weeks. The retinal blood flow and neural activity were assessed using laser speckle flowgraphy and electroretinography (ERG), respectively. The db/db mice had significantly higher blood glucose levels and body weight. The resting retinal blood flow was steady and comparable between two groups throughout the study. Hyperoxia elicited a consistent decrease, and flicker light an increase, in retinal blood flow in db/m mice independent of age. However, these flow responses were significantly diminished in db/db mice at 8 weeks old and then the mice became unresponsive to stimulations at 12 weeks. Subsequently, the ERG implicit time for oscillatory potential was significantly increased at 14 weeks of age while the a-wave and b-wave amplitudes and implicit times remained unchanged. The deficiencies of flow regulation and neurovascular coupling in the retina appear to precede neural dysfunction in the mouse with type 2 diabetes.
format	article
author	Junya Hanaguri Harumasa Yokota Masahisa Watanabe Satoru Yamagami Akifumi Kushiyama Lih Kuo Taiji Nagaoka
author_facet	Junya Hanaguri Harumasa Yokota Masahisa Watanabe Satoru Yamagami Akifumi Kushiyama Lih Kuo Taiji Nagaoka
author_sort	Junya Hanaguri
title	Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
title_short	Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
title_full	Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
title_fullStr	Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
title_full_unstemmed	Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
title_sort	retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/02862ec994d94060ad2eb7b8803bd4e0
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Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice

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