Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet

Reactive oxygen species (ROS) have been subject of increasing interest in the pathophysiology and therapy of cancers in recent years. In skin cancer, ROS are involved in UV-induced tumorigenesis and its targeted treatment via, e.g., photodynamic therapy. Another recent technology for topical ROS gen...

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Autores principales:	Fariba Saadati, Juliane Moritz, Julia Berner, Eric Freund, Lea Miebach, Iris Helfrich, Ingo Stoffels, Steffen Emmert, Sander Bekeschus
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chemokines cold physical plasma cytokines reactive oxygen species ROS skin cancer Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/02876ad57ddd43a6886cc9631a13d2c9
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spelling	oai:doaj.org-article:02876ad57ddd43a6886cc9631a13d2c92021-11-11T16:54:36ZPatient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet10.3390/ijms2221114461422-00671661-6596https://doaj.org/article/02876ad57ddd43a6886cc9631a13d2c92021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11446https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Reactive oxygen species (ROS) have been subject of increasing interest in the pathophysiology and therapy of cancers in recent years. In skin cancer, ROS are involved in UV-induced tumorigenesis and its targeted treatment via, e.g., photodynamic therapy. Another recent technology for topical ROS generation is cold physical plasma, a partially ionized gas expelling dozens of reactive species onto its treatment target. Gas plasma technology is accredited for its wound-healing abilities in Europe, and current clinical evidence suggests that it may have beneficial effects against actinic keratosis. Since the concept of hormesis dictates that low ROS levels perform signaling functions, while high ROS levels cause damage, we investigated herein the antitumor activity of gas plasma in non-melanoma skin cancer. In vitro, gas plasma exposure diminished the metabolic activity, preferentially in squamous cell carcinoma cell (SCC) lines compared to non-malignant HaCaT cells. In patient-derived basal cell carcinoma (BCC) and SCC samples treated with gas plasma ex vivo, increased apoptosis was found in both cancer types. Moreover, the immunomodulatory actions of gas plasma treatment were found affecting, e.g., the expression of CD86 and the number of regulatory T-cells. The supernatants of these ex vivo cultured tumors were quantitatively screened for cytokines, chemokines, and growth factors, identifying CCL5 and GM-CSF, molecules associated with skin cancer metastasis, to be markedly decreased. These findings suggest gas plasma treatment to be an interesting future technology for non-melanoma skin cancer topical therapy.Fariba SaadatiJuliane MoritzJulia BernerEric FreundLea MiebachIris HelfrichIngo StoffelsSteffen EmmertSander BekeschusMDPI AGarticlechemokinescold physical plasmacytokinesreactive oxygen speciesROSskin cancerBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11446, p 11446 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chemokines cold physical plasma cytokines reactive oxygen species ROS skin cancer Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	chemokines cold physical plasma cytokines reactive oxygen species ROS skin cancer Biology (General) QH301-705.5 Chemistry QD1-999 Fariba Saadati Juliane Moritz Julia Berner Eric Freund Lea Miebach Iris Helfrich Ingo Stoffels Steffen Emmert Sander Bekeschus Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
description	Reactive oxygen species (ROS) have been subject of increasing interest in the pathophysiology and therapy of cancers in recent years. In skin cancer, ROS are involved in UV-induced tumorigenesis and its targeted treatment via, e.g., photodynamic therapy. Another recent technology for topical ROS generation is cold physical plasma, a partially ionized gas expelling dozens of reactive species onto its treatment target. Gas plasma technology is accredited for its wound-healing abilities in Europe, and current clinical evidence suggests that it may have beneficial effects against actinic keratosis. Since the concept of hormesis dictates that low ROS levels perform signaling functions, while high ROS levels cause damage, we investigated herein the antitumor activity of gas plasma in non-melanoma skin cancer. In vitro, gas plasma exposure diminished the metabolic activity, preferentially in squamous cell carcinoma cell (SCC) lines compared to non-malignant HaCaT cells. In patient-derived basal cell carcinoma (BCC) and SCC samples treated with gas plasma ex vivo, increased apoptosis was found in both cancer types. Moreover, the immunomodulatory actions of gas plasma treatment were found affecting, e.g., the expression of CD86 and the number of regulatory T-cells. The supernatants of these ex vivo cultured tumors were quantitatively screened for cytokines, chemokines, and growth factors, identifying CCL5 and GM-CSF, molecules associated with skin cancer metastasis, to be markedly decreased. These findings suggest gas plasma treatment to be an interesting future technology for non-melanoma skin cancer topical therapy.
format	article
author	Fariba Saadati Juliane Moritz Julia Berner Eric Freund Lea Miebach Iris Helfrich Ingo Stoffels Steffen Emmert Sander Bekeschus
author_facet	Fariba Saadati Juliane Moritz Julia Berner Eric Freund Lea Miebach Iris Helfrich Ingo Stoffels Steffen Emmert Sander Bekeschus
author_sort	Fariba Saadati
title	Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
title_short	Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
title_full	Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
title_fullStr	Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
title_full_unstemmed	Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet
title_sort	patient-derived human basal and cutaneous squamous cell carcinoma tissues display apoptosis and immunomodulation following gas plasma exposure with a certified argon jet
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/02876ad57ddd43a6886cc9631a13d2c9
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Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet

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