Applications of CRISPR-Cas Technologies to Proteomics

CRISPR-Cas-based genome editing is a revolutionary approach that has provided an unprecedented investigational power for the life sciences. Rapid and efficient, CRISPR-Cas technologies facilitate the generation of complex biological models and at the same time provide the necessary methods required...

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Autores principales:	Georgii Dolgalev, Ekaterina Poverennaya
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	proteomics CRISPR-Cas genome editing interactomics Genetics QH426-470
Acceso en línea:	https://doaj.org/article/028d280c503e41bf80c114bba4a1e987
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spelling	oai:doaj.org-article:028d280c503e41bf80c114bba4a1e9872021-11-25T17:42:00ZApplications of CRISPR-Cas Technologies to Proteomics10.3390/genes121117902073-4425https://doaj.org/article/028d280c503e41bf80c114bba4a1e9872021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1790https://doaj.org/toc/2073-4425CRISPR-Cas-based genome editing is a revolutionary approach that has provided an unprecedented investigational power for the life sciences. Rapid and efficient, CRISPR-Cas technologies facilitate the generation of complex biological models and at the same time provide the necessary methods required to study these models in depth. The field of proteomics has already significantly benefited from leveraging the power of CRISPR-Cas technologies, however, many potential applications of these technologies in the context of proteomics remain unexplored. In this review, we intend to provide an introduction to the CRISPR-Cas technologies and demonstrate how they can be applied to solving proteome-centric questions. To achieve this goal, we begin with the description of the modern suite of CRISPR-Cas-based tools, focusing on the more mature CRISPR-Cas9 system. In the second part of this review, we highlight both established and potential applications of the CRISPR-Cas technologies to proteomics.Georgii DolgalevEkaterina PoverennayaMDPI AGarticleproteomicsCRISPR-Casgenome editinginteractomicsGeneticsQH426-470ENGenes, Vol 12, Iss 1790, p 1790 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proteomics CRISPR-Cas genome editing interactomics Genetics QH426-470
spellingShingle	proteomics CRISPR-Cas genome editing interactomics Genetics QH426-470 Georgii Dolgalev Ekaterina Poverennaya Applications of CRISPR-Cas Technologies to Proteomics
description	CRISPR-Cas-based genome editing is a revolutionary approach that has provided an unprecedented investigational power for the life sciences. Rapid and efficient, CRISPR-Cas technologies facilitate the generation of complex biological models and at the same time provide the necessary methods required to study these models in depth. The field of proteomics has already significantly benefited from leveraging the power of CRISPR-Cas technologies, however, many potential applications of these technologies in the context of proteomics remain unexplored. In this review, we intend to provide an introduction to the CRISPR-Cas technologies and demonstrate how they can be applied to solving proteome-centric questions. To achieve this goal, we begin with the description of the modern suite of CRISPR-Cas-based tools, focusing on the more mature CRISPR-Cas9 system. In the second part of this review, we highlight both established and potential applications of the CRISPR-Cas technologies to proteomics.
format	article
author	Georgii Dolgalev Ekaterina Poverennaya
author_facet	Georgii Dolgalev Ekaterina Poverennaya
author_sort	Georgii Dolgalev
title	Applications of CRISPR-Cas Technologies to Proteomics
title_short	Applications of CRISPR-Cas Technologies to Proteomics
title_full	Applications of CRISPR-Cas Technologies to Proteomics
title_fullStr	Applications of CRISPR-Cas Technologies to Proteomics
title_full_unstemmed	Applications of CRISPR-Cas Technologies to Proteomics
title_sort	applications of crispr-cas technologies to proteomics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/028d280c503e41bf80c114bba4a1e987
work_keys_str_mv	AT georgiidolgalev applicationsofcrisprcastechnologiestoproteomics AT ekaterinapoverennaya applicationsofcrisprcastechnologiestoproteomics
_version_	1718412085017182208

Applications of CRISPR-Cas Technologies to Proteomics

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