Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice

Long-chain omega-3 polyunsaturated fatty acids (LC-PUFAs), known for having many health benefits, are usually present in three forms: triglycerides (TG), ethyl esters (EE), and phospholipid (PL). In this study, the effects of these three LC-PUFAs forms (fish oil for TG and EE, krill oil for PL) on t...

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Autores principales:	Jiaping Pan, Huan Wang, Ye Li, Chenyang Lu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	LC-PUFAs Fish oil Krill oil Label-free quantitative proteomics Lipids metabolism Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/028e064756034e48ba2a1c74f265fe9f
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spelling	oai:doaj.org-article:028e064756034e48ba2a1c74f265fe9f2021-11-28T04:31:49ZComparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice2213-453010.1016/j.fshw.2021.11.005https://doaj.org/article/028e064756034e48ba2a1c74f265fe9f2022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213453021001002https://doaj.org/toc/2213-4530Long-chain omega-3 polyunsaturated fatty acids (LC-PUFAs), known for having many health benefits, are usually present in three forms: triglycerides (TG), ethyl esters (EE), and phospholipid (PL). In this study, the effects of these three LC-PUFAs forms (fish oil for TG and EE, krill oil for PL) on the obese mice were compared, and the proteomic changes that focused on lipid metabolism were evaluated via label-free quantitative proteomics analysis. Compared with the model group, all three of the LC-PUFA form supplementations (labeled as the FO-TG group, FO-EE group and KO-PL groups) could significantly reduce body weight gain (P < 0.01). Low-density lipoprotein cholesterol levels were significantly decreased, whereas high-density lipoprotein cholesterol levels were significantly increased in the FO-TG group and FO-EE group (P < 0.01), and especially in the PL group (P < 0.001). Furthermore, proteomics analysis results suggested that some differentially expressed genes involved in the fatty acid degradation and oxidation pathways had a higher expression fold in the KO-PL group than in the FO-TG or FO-EE groups. Our results showed that dietary LC-PUFAs can reduce fat deposition and inhibit lipogenesis in the liver by upregulating the expression of proteins that are involved in the fatty acid degradation and oxidation pathways. Additionally, KO-PL elicits stronger effects than FO-TG or FO-EE.Jiaping PanHuan WangYe LiChenyang LuKeAi Communications Co., Ltd.articleLC-PUFAsFish oilKrill oilLabel-free quantitative proteomicsLipids metabolismNutrition. Foods and food supplyTX341-641ENFood Science and Human Wellness, Vol 11, Iss 2, Pp 316-322 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	LC-PUFAs Fish oil Krill oil Label-free quantitative proteomics Lipids metabolism Nutrition. Foods and food supply TX341-641
spellingShingle	LC-PUFAs Fish oil Krill oil Label-free quantitative proteomics Lipids metabolism Nutrition. Foods and food supply TX341-641 Jiaping Pan Huan Wang Ye Li Chenyang Lu Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
description	Long-chain omega-3 polyunsaturated fatty acids (LC-PUFAs), known for having many health benefits, are usually present in three forms: triglycerides (TG), ethyl esters (EE), and phospholipid (PL). In this study, the effects of these three LC-PUFAs forms (fish oil for TG and EE, krill oil for PL) on the obese mice were compared, and the proteomic changes that focused on lipid metabolism were evaluated via label-free quantitative proteomics analysis. Compared with the model group, all three of the LC-PUFA form supplementations (labeled as the FO-TG group, FO-EE group and KO-PL groups) could significantly reduce body weight gain (P < 0.01). Low-density lipoprotein cholesterol levels were significantly decreased, whereas high-density lipoprotein cholesterol levels were significantly increased in the FO-TG group and FO-EE group (P < 0.01), and especially in the PL group (P < 0.001). Furthermore, proteomics analysis results suggested that some differentially expressed genes involved in the fatty acid degradation and oxidation pathways had a higher expression fold in the KO-PL group than in the FO-TG or FO-EE groups. Our results showed that dietary LC-PUFAs can reduce fat deposition and inhibit lipogenesis in the liver by upregulating the expression of proteins that are involved in the fatty acid degradation and oxidation pathways. Additionally, KO-PL elicits stronger effects than FO-TG or FO-EE.
format	article
author	Jiaping Pan Huan Wang Ye Li Chenyang Lu
author_facet	Jiaping Pan Huan Wang Ye Li Chenyang Lu
author_sort	Jiaping Pan
title	Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
title_short	Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
title_full	Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
title_fullStr	Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
title_full_unstemmed	Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
title_sort	comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/028e064756034e48ba2a1c74f265fe9f
work_keys_str_mv	AT jiapingpan comparativeproteomicanalysisrevealstheeffectsofdifferentfattyacidformsonhighfatdietmice AT huanwang comparativeproteomicanalysisrevealstheeffectsofdifferentfattyacidformsonhighfatdietmice AT yeli comparativeproteomicanalysisrevealstheeffectsofdifferentfattyacidformsonhighfatdietmice AT chenyanglu comparativeproteomicanalysisrevealstheeffectsofdifferentfattyacidformsonhighfatdietmice
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Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice

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