<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>

The ability of Streptococcus pneumoniae to induce infections relies on its virulence factor machinery. A previous CRISPR interference (CRISPRi) study had identified 254 essential proteins that may be responsible towards the pathogenicity of S. pneumoniae serotype 2 strain D39. However, 39 of them we...

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Autores principales:	Khairiah Razali, Azzmer Azzar Abdul Hamid, Noor Hasniza Md Zin, Noraslinda Muhamad Bunnori, Hanani Ahmad Yusof, Kamarul Rahim Kamarudin, Aisyah Mohamed Rehan
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2020
Materias:	hypothetical proteins s. pneumoniae strain d39 in silico analysis of protein bioinformatics tools Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/028fdc1b286b4b89aca4501a345b7ae4
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spelling	oai:doaj.org-article:028fdc1b286b4b89aca4501a345b7ae42021-12-02T13:16:24Z<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>1411-94202460-157810.22146/ijc.41817https://doaj.org/article/028fdc1b286b4b89aca4501a345b7ae42020-03-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/41817https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578The ability of Streptococcus pneumoniae to induce infections relies on its virulence factor machinery. A previous CRISPR interference (CRISPRi) study had identified 254 essential proteins that may be responsible towards the pathogenicity of S. pneumoniae serotype 2 strain D39. However, 39 of them were functionally and structurally uncharacterized. Hence, by using in silico approach, this study aimed to annotate the function and structure of these un-annotated proteins. Initially, all 39 proteins went through primary screening for template availability and pathogenicity. From there, 11 of them were selected and underwent further physicochemical, functional and structural categorization through integrated bioinformatics approach by means of amino acid sequence- and structure- based analyses. The obtained data revealed that 9 targeted proteins showed high possibility to be involved in either cell viability or cell pathogenicity mechanism of the bacterium, with SPD_1333 and SPD_1743 being the two most promising proteins to be further studied. Findings from this study can help in facilitating a better understanding of pathogenic ability of this microorganism and enhance drug development and target identification processes in the aim of improving pneumococcal disease control.Khairiah RazaliAzzmer Azzar Abdul HamidNoor Hasniza Md ZinNoraslinda Muhamad BunnoriHanani Ahmad YusofKamarul Rahim KamarudinAisyah Mohamed RehanDepartment of Chemistry, Universitas Gadjah Madaarticlehypothetical proteinss. pneumoniae strain d39in silico analysis of proteinbioinformatics toolsChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 20, Iss 2, Pp 336-347 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypothetical proteins s. pneumoniae strain d39 in silico analysis of protein bioinformatics tools Chemistry QD1-999
spellingShingle	hypothetical proteins s. pneumoniae strain d39 in silico analysis of protein bioinformatics tools Chemistry QD1-999 Khairiah Razali Azzmer Azzar Abdul Hamid Noor Hasniza Md Zin Noraslinda Muhamad Bunnori Hanani Ahmad Yusof Kamarul Rahim Kamarudin Aisyah Mohamed Rehan <i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>
description	The ability of Streptococcus pneumoniae to induce infections relies on its virulence factor machinery. A previous CRISPR interference (CRISPRi) study had identified 254 essential proteins that may be responsible towards the pathogenicity of S. pneumoniae serotype 2 strain D39. However, 39 of them were functionally and structurally uncharacterized. Hence, by using in silico approach, this study aimed to annotate the function and structure of these un-annotated proteins. Initially, all 39 proteins went through primary screening for template availability and pathogenicity. From there, 11 of them were selected and underwent further physicochemical, functional and structural categorization through integrated bioinformatics approach by means of amino acid sequence- and structure- based analyses. The obtained data revealed that 9 targeted proteins showed high possibility to be involved in either cell viability or cell pathogenicity mechanism of the bacterium, with SPD_1333 and SPD_1743 being the two most promising proteins to be further studied. Findings from this study can help in facilitating a better understanding of pathogenic ability of this microorganism and enhance drug development and target identification processes in the aim of improving pneumococcal disease control.
format	article
author	Khairiah Razali Azzmer Azzar Abdul Hamid Noor Hasniza Md Zin Noraslinda Muhamad Bunnori Hanani Ahmad Yusof Kamarul Rahim Kamarudin Aisyah Mohamed Rehan
author_facet	Khairiah Razali Azzmer Azzar Abdul Hamid Noor Hasniza Md Zin Noraslinda Muhamad Bunnori Hanani Ahmad Yusof Kamarul Rahim Kamarudin Aisyah Mohamed Rehan
author_sort	Khairiah Razali
title	<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>
title_short	<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>
title_full	<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>
title_fullStr	<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>
title_full_unstemmed	<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>
title_sort	<i>in silico</i> structural and functional annotation of nine essential hypothetical proteins from <i>streptococcus pneumoniae</i>
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/028fdc1b286b4b89aca4501a345b7ae4
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<i>In Silico</i> Structural and Functional Annotation of Nine Essential Hypothetical Proteins from <i>Streptococcus pneumoniae</i>

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