Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>

Unlike transcriptional regulation, the post-transcriptional mechanisms underlying zygotic segmentation gene expression in early <i>Drosophila</i> embryo have been insufficiently investigated. Condition-specific post-transcriptional regulation plays an important role in the development of...

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Autores principales:	Maria A. Duk, Vitaly V. Gursky, Maria G. Samsonova, Svetlana Yu. Surkova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Drosophila</i> embryo segmentation genes post-transcriptional regulation gene expression pattern formation dynamical model Science Q
Acceso en línea:	https://doaj.org/article/029960948e934336b464627afb00d077
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spelling	oai:doaj.org-article:029960948e934336b464627afb00d0772021-11-25T18:11:23ZApplication of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>10.3390/life111112322075-1729https://doaj.org/article/029960948e934336b464627afb00d0772021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1232https://doaj.org/toc/2075-1729Unlike transcriptional regulation, the post-transcriptional mechanisms underlying zygotic segmentation gene expression in early <i>Drosophila</i> embryo have been insufficiently investigated. Condition-specific post-transcriptional regulation plays an important role in the development of many organisms. Our recent study revealed the domain- and genotype-specific differences between mRNA and the protein expression of <i>Drosophila hb</i>, <i>gt</i>, and <i>eve</i> genes in cleavage cycle 14A. Here, we use this dataset and the dynamic mathematical model to recapitulate protein expression from the corresponding mRNA patterns. The condition-specific nonuniformity in parameter values is further interpreted in terms of possible post-transcriptional modifications. For <i>hb</i> expression in wild-type embryos, our results predict the position-specific differences in protein production. The protein synthesis rate parameter is significantly higher in <i>hb</i> anterior domain compared to the posterior domain. The parameter sets describing Gt protein dynamics in wild-type embryos and <i>Kr</i> mutants are genotype-specific. The spatial discrepancy between <i>gt</i> mRNA and protein posterior expression in <i>Kr</i> mutants is well reproduced by the whole axis model, thus rejecting the involvement of post-transcriptional mechanisms. Our models fail to describe the full dynamics of <i>eve</i> expression, presumably due to its complex shape and the variable time delays between mRNA and protein patterns, which likely require a more complex model. Overall, our modeling approach enables the prediction of regulatory scenarios underlying the condition-specific differences between mRNA and protein expression in early embryo.Maria A. DukVitaly V. GurskyMaria G. SamsonovaSvetlana Yu. SurkovaMDPI AGarticle<i>Drosophila</i> embryosegmentation genespost-transcriptional regulationgene expressionpattern formationdynamical modelScienceQENLife, Vol 11, Iss 1232, p 1232 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Drosophila</i> embryo segmentation genes post-transcriptional regulation gene expression pattern formation dynamical model Science Q
spellingShingle	<i>Drosophila</i> embryo segmentation genes post-transcriptional regulation gene expression pattern formation dynamical model Science Q Maria A. Duk Vitaly V. Gursky Maria G. Samsonova Svetlana Yu. Surkova Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>
description	Unlike transcriptional regulation, the post-transcriptional mechanisms underlying zygotic segmentation gene expression in early <i>Drosophila</i> embryo have been insufficiently investigated. Condition-specific post-transcriptional regulation plays an important role in the development of many organisms. Our recent study revealed the domain- and genotype-specific differences between mRNA and the protein expression of <i>Drosophila hb</i>, <i>gt</i>, and <i>eve</i> genes in cleavage cycle 14A. Here, we use this dataset and the dynamic mathematical model to recapitulate protein expression from the corresponding mRNA patterns. The condition-specific nonuniformity in parameter values is further interpreted in terms of possible post-transcriptional modifications. For <i>hb</i> expression in wild-type embryos, our results predict the position-specific differences in protein production. The protein synthesis rate parameter is significantly higher in <i>hb</i> anterior domain compared to the posterior domain. The parameter sets describing Gt protein dynamics in wild-type embryos and <i>Kr</i> mutants are genotype-specific. The spatial discrepancy between <i>gt</i> mRNA and protein posterior expression in <i>Kr</i> mutants is well reproduced by the whole axis model, thus rejecting the involvement of post-transcriptional mechanisms. Our models fail to describe the full dynamics of <i>eve</i> expression, presumably due to its complex shape and the variable time delays between mRNA and protein patterns, which likely require a more complex model. Overall, our modeling approach enables the prediction of regulatory scenarios underlying the condition-specific differences between mRNA and protein expression in early embryo.
format	article
author	Maria A. Duk Vitaly V. Gursky Maria G. Samsonova Svetlana Yu. Surkova
author_facet	Maria A. Duk Vitaly V. Gursky Maria G. Samsonova Svetlana Yu. Surkova
author_sort	Maria A. Duk
title	Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>
title_short	Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>
title_full	Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>
title_fullStr	Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>
title_full_unstemmed	Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>
title_sort	application of domain- and genotype-specific models to infer post-transcriptional regulation of segmentation gene expression in <i>drosophila</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/029960948e934336b464627afb00d077
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_version_	1718411480069570560

Application of Domain- and Genotype-Specific Models to Infer Post-Transcriptional Regulation of Segmentation Gene Expression in <i>Drosophila</i>

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