Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Abstract Background Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on reproducible and effective propagation protocol while retaining the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saikat Gantait, Manisha Mahanta
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Auxin Callogenesis Indirect organogenesis Molecular marker Multiple shoots Survival Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/029a67a7e29e4827b0446e7005afba1e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:029a67a7e29e4827b0446e7005afba1e
record_format	dspace
spelling	oai:doaj.org-article:029a67a7e29e4827b0446e7005afba1e2021-11-21T12:33:04ZPicloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera10.1186/s43141-021-00269-12090-5920https://doaj.org/article/029a67a7e29e4827b0446e7005afba1e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s43141-021-00269-1https://doaj.org/toc/2090-5920Abstract Background Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on reproducible and effective propagation protocol while retaining the genetic uniformity of G. jamesonii. The present study, for the first time, reports the potential of picloram for enhanced induction of organogenic calli from leaves of G. jamesonii and its high-frequency indirect regeneration. Results The fastest induction of calli with maximum fresh and dry weight was recorded in the Murashige and Skoog (MS) semisolid medium supplemented with 1 mg/l picloram. In addition, callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight. The proliferated calli were transferred to shoot induction media containing MS salt and 0.5–1 mg/l N6-benzylaminopurine, kinetin, or thidiazuron. A mean number of ~6 shoots per callus were developed after 5 days of culture in the MS medium supplemented with 1 mg/l kinetin, with a mean length of 5.2 cm. Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded. Complete plantlets were primarily acclimatized in sand before being transferred to a mixed substrate (of soil, sand, tea leaf waste, and cow urine) that secured >90% survival and further growth of the plantlets. Eventually, clonal fidelity of the in vitro regenerants assessed via inter-simple sequence repeats (ISSR) primers exhibited a monomorphic banding patterns that suggested genetic integrity within the plantlets as well as with their mother plant. Conclusions The results of the present study should be of interest for commercial propagation and mutagenesis- as well as genetic transformation-related research.Saikat GantaitManisha MahantaSpringerOpenarticleAuxinCallogenesisIndirect organogenesisMolecular markerMultiple shootsSurvivalBiotechnologyTP248.13-248.65GeneticsQH426-470ENJournal of Genetic Engineering and Biotechnology, Vol 19, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Auxin Callogenesis Indirect organogenesis Molecular marker Multiple shoots Survival Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Auxin Callogenesis Indirect organogenesis Molecular marker Multiple shoots Survival Biotechnology TP248.13-248.65 Genetics QH426-470 Saikat Gantait Manisha Mahanta Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
description	Abstract Background Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on reproducible and effective propagation protocol while retaining the genetic uniformity of G. jamesonii. The present study, for the first time, reports the potential of picloram for enhanced induction of organogenic calli from leaves of G. jamesonii and its high-frequency indirect regeneration. Results The fastest induction of calli with maximum fresh and dry weight was recorded in the Murashige and Skoog (MS) semisolid medium supplemented with 1 mg/l picloram. In addition, callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight. The proliferated calli were transferred to shoot induction media containing MS salt and 0.5–1 mg/l N6-benzylaminopurine, kinetin, or thidiazuron. A mean number of ~6 shoots per callus were developed after 5 days of culture in the MS medium supplemented with 1 mg/l kinetin, with a mean length of 5.2 cm. Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded. Complete plantlets were primarily acclimatized in sand before being transferred to a mixed substrate (of soil, sand, tea leaf waste, and cow urine) that secured >90% survival and further growth of the plantlets. Eventually, clonal fidelity of the in vitro regenerants assessed via inter-simple sequence repeats (ISSR) primers exhibited a monomorphic banding patterns that suggested genetic integrity within the plantlets as well as with their mother plant. Conclusions The results of the present study should be of interest for commercial propagation and mutagenesis- as well as genetic transformation-related research.
format	article
author	Saikat Gantait Manisha Mahanta
author_facet	Saikat Gantait Manisha Mahanta
author_sort	Saikat Gantait
title	Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
title_short	Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
title_full	Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
title_fullStr	Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
title_full_unstemmed	Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
title_sort	picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/029a67a7e29e4827b0446e7005afba1e
work_keys_str_mv	AT saikatgantait picloraminducedenhancedcallusmediatedregenerationacclimatizationandgeneticclonalityassessmentofgerbera AT manishamahanta picloraminducedenhancedcallusmediatedregenerationacclimatizationandgeneticclonalityassessmentofgerbera
_version_	1718418924136038400

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Ejemplares similares