Raman image-activated cell sorting

Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no need for labeling.

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Autores principales: Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/02b0d5e6a65b4b81bf87a4cb06c904e6
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spelling oai:doaj.org-article:02b0d5e6a65b4b81bf87a4cb06c904e62021-12-02T16:24:52ZRaman image-activated cell sorting10.1038/s41467-020-17285-32041-1723https://doaj.org/article/02b0d5e6a65b4b81bf87a4cb06c904e62020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17285-3https://doaj.org/toc/2041-1723Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no need for labeling.Nao NittaTakanori IinoAkihiro IsozakiMai YamagishiYasutaka KitahamaShinya SakumaYuta SuzukiHiroshi TezukaMinoru OikawaFumihito AraiTakuya AsaiDinghuan DengHideya FukuzawaMisa HaseTomohisa HasunumaTakeshi HayakawaKei HirakiKotaro HiramatsuYu HoshinoMary InabaYuki InoueTakuro ItoMasataka KajikawaHiroshi KarakawaYusuke KasaiYuichi KatoHirofumi KobayashiCheng LeiSatoshi MatsusakaHideharu MikamiAtsuhiro NakagawaKeiji NumataTadataka OtaTakeichiro SekiyaKiyotaka ShibaYoshitaka ShirasakiNobutake SuzukiShunji TanakaShunnosuke UenoHiroshi WataraiTakashi YamanoMasayuki YazawaYusuke YonamineDino Di CarloYoichiroh HosokawaSotaro UemuraTakeaki SugimuraYasuyuki OzekiKeisuke GodaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-16 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Nao Nitta
Takanori Iino
Akihiro Isozaki
Mai Yamagishi
Yasutaka Kitahama
Shinya Sakuma
Yuta Suzuki
Hiroshi Tezuka
Minoru Oikawa
Fumihito Arai
Takuya Asai
Dinghuan Deng
Hideya Fukuzawa
Misa Hase
Tomohisa Hasunuma
Takeshi Hayakawa
Kei Hiraki
Kotaro Hiramatsu
Yu Hoshino
Mary Inaba
Yuki Inoue
Takuro Ito
Masataka Kajikawa
Hiroshi Karakawa
Yusuke Kasai
Yuichi Kato
Hirofumi Kobayashi
Cheng Lei
Satoshi Matsusaka
Hideharu Mikami
Atsuhiro Nakagawa
Keiji Numata
Tadataka Ota
Takeichiro Sekiya
Kiyotaka Shiba
Yoshitaka Shirasaki
Nobutake Suzuki
Shunji Tanaka
Shunnosuke Ueno
Hiroshi Watarai
Takashi Yamano
Masayuki Yazawa
Yusuke Yonamine
Dino Di Carlo
Yoichiroh Hosokawa
Sotaro Uemura
Takeaki Sugimura
Yasuyuki Ozeki
Keisuke Goda
Raman image-activated cell sorting
description Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no need for labeling.
format article
author Nao Nitta
Takanori Iino
Akihiro Isozaki
Mai Yamagishi
Yasutaka Kitahama
Shinya Sakuma
Yuta Suzuki
Hiroshi Tezuka
Minoru Oikawa
Fumihito Arai
Takuya Asai
Dinghuan Deng
Hideya Fukuzawa
Misa Hase
Tomohisa Hasunuma
Takeshi Hayakawa
Kei Hiraki
Kotaro Hiramatsu
Yu Hoshino
Mary Inaba
Yuki Inoue
Takuro Ito
Masataka Kajikawa
Hiroshi Karakawa
Yusuke Kasai
Yuichi Kato
Hirofumi Kobayashi
Cheng Lei
Satoshi Matsusaka
Hideharu Mikami
Atsuhiro Nakagawa
Keiji Numata
Tadataka Ota
Takeichiro Sekiya
Kiyotaka Shiba
Yoshitaka Shirasaki
Nobutake Suzuki
Shunji Tanaka
Shunnosuke Ueno
Hiroshi Watarai
Takashi Yamano
Masayuki Yazawa
Yusuke Yonamine
Dino Di Carlo
Yoichiroh Hosokawa
Sotaro Uemura
Takeaki Sugimura
Yasuyuki Ozeki
Keisuke Goda
author_facet Nao Nitta
Takanori Iino
Akihiro Isozaki
Mai Yamagishi
Yasutaka Kitahama
Shinya Sakuma
Yuta Suzuki
Hiroshi Tezuka
Minoru Oikawa
Fumihito Arai
Takuya Asai
Dinghuan Deng
Hideya Fukuzawa
Misa Hase
Tomohisa Hasunuma
Takeshi Hayakawa
Kei Hiraki
Kotaro Hiramatsu
Yu Hoshino
Mary Inaba
Yuki Inoue
Takuro Ito
Masataka Kajikawa
Hiroshi Karakawa
Yusuke Kasai
Yuichi Kato
Hirofumi Kobayashi
Cheng Lei
Satoshi Matsusaka
Hideharu Mikami
Atsuhiro Nakagawa
Keiji Numata
Tadataka Ota
Takeichiro Sekiya
Kiyotaka Shiba
Yoshitaka Shirasaki
Nobutake Suzuki
Shunji Tanaka
Shunnosuke Ueno
Hiroshi Watarai
Takashi Yamano
Masayuki Yazawa
Yusuke Yonamine
Dino Di Carlo
Yoichiroh Hosokawa
Sotaro Uemura
Takeaki Sugimura
Yasuyuki Ozeki
Keisuke Goda
author_sort Nao Nitta
title Raman image-activated cell sorting
title_short Raman image-activated cell sorting
title_full Raman image-activated cell sorting
title_fullStr Raman image-activated cell sorting
title_full_unstemmed Raman image-activated cell sorting
title_sort raman image-activated cell sorting
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/02b0d5e6a65b4b81bf87a4cb06c904e6
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