Dependence of micelle size and shape on detergent alkyl chain length and head group.

Micelle-forming detergents provide an amphipathic environment that can mimic lipid bilayers and are important tools for solubilizing membrane proteins for functional and structural investigations in vitro. However, the formation of a soluble protein-detergent complex (PDC) currently relies on empiri...

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Autores principales:	Ryan C Oliver, Jan Lipfert, Daniel A Fox, Ryan H Lo, Sebastian Doniach, Linda Columbus
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02b3a887fed24b79bca725e761343091
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spelling	oai:doaj.org-article:02b3a887fed24b79bca725e7613430912021-11-18T07:46:22ZDependence of micelle size and shape on detergent alkyl chain length and head group.1932-620310.1371/journal.pone.0062488https://doaj.org/article/02b3a887fed24b79bca725e7613430912013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23667481/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Micelle-forming detergents provide an amphipathic environment that can mimic lipid bilayers and are important tools for solubilizing membrane proteins for functional and structural investigations in vitro. However, the formation of a soluble protein-detergent complex (PDC) currently relies on empirical screening of detergents, and a stable and functional PDC is often not obtained. To provide a foundation for systematic comparisons between the properties of the detergent micelle and the resulting PDC, a comprehensive set of detergents commonly used for membrane protein studies are systematically investigated. Using small-angle X-ray scattering (SAXS), micelle shapes and sizes are determined for phosphocholines with 10, 12, and 14 alkyl carbons, glucosides with 8, 9, and 10 alkyl carbons, maltosides with 8, 10, and 12 alkyl carbons, and lysophosphatidyl glycerols with 14 and 16 alkyl carbons. The SAXS profiles are well described by two-component ellipsoid models, with an electron rich outer shell corresponding to the detergent head groups and a less electron dense hydrophobic core composed of the alkyl chains. The minor axis of the elliptical micelle core from these models is constrained by the length of the alkyl chain, and increases by 1.2-1.5 Å per carbon addition to the alkyl chain. The major elliptical axis also increases with chain length; however, the ellipticity remains approximately constant for each detergent series. In addition, the aggregation number of these detergents increases by ∼16 monomers per micelle for each alkyl carbon added. The data provide a comprehensive view of the determinants of micelle shape and size and provide a baseline for correlating micelle properties with protein-detergent interactions.Ryan C OliverJan LipfertDaniel A FoxRyan H LoSebastian DoniachLinda ColumbusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62488 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan C Oliver Jan Lipfert Daniel A Fox Ryan H Lo Sebastian Doniach Linda Columbus Dependence of micelle size and shape on detergent alkyl chain length and head group.
description	Micelle-forming detergents provide an amphipathic environment that can mimic lipid bilayers and are important tools for solubilizing membrane proteins for functional and structural investigations in vitro. However, the formation of a soluble protein-detergent complex (PDC) currently relies on empirical screening of detergents, and a stable and functional PDC is often not obtained. To provide a foundation for systematic comparisons between the properties of the detergent micelle and the resulting PDC, a comprehensive set of detergents commonly used for membrane protein studies are systematically investigated. Using small-angle X-ray scattering (SAXS), micelle shapes and sizes are determined for phosphocholines with 10, 12, and 14 alkyl carbons, glucosides with 8, 9, and 10 alkyl carbons, maltosides with 8, 10, and 12 alkyl carbons, and lysophosphatidyl glycerols with 14 and 16 alkyl carbons. The SAXS profiles are well described by two-component ellipsoid models, with an electron rich outer shell corresponding to the detergent head groups and a less electron dense hydrophobic core composed of the alkyl chains. The minor axis of the elliptical micelle core from these models is constrained by the length of the alkyl chain, and increases by 1.2-1.5 Å per carbon addition to the alkyl chain. The major elliptical axis also increases with chain length; however, the ellipticity remains approximately constant for each detergent series. In addition, the aggregation number of these detergents increases by ∼16 monomers per micelle for each alkyl carbon added. The data provide a comprehensive view of the determinants of micelle shape and size and provide a baseline for correlating micelle properties with protein-detergent interactions.
format	article
author	Ryan C Oliver Jan Lipfert Daniel A Fox Ryan H Lo Sebastian Doniach Linda Columbus
author_facet	Ryan C Oliver Jan Lipfert Daniel A Fox Ryan H Lo Sebastian Doniach Linda Columbus
author_sort	Ryan C Oliver
title	Dependence of micelle size and shape on detergent alkyl chain length and head group.
title_short	Dependence of micelle size and shape on detergent alkyl chain length and head group.
title_full	Dependence of micelle size and shape on detergent alkyl chain length and head group.
title_fullStr	Dependence of micelle size and shape on detergent alkyl chain length and head group.
title_full_unstemmed	Dependence of micelle size and shape on detergent alkyl chain length and head group.
title_sort	dependence of micelle size and shape on detergent alkyl chain length and head group.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/02b3a887fed24b79bca725e761343091
work_keys_str_mv	AT ryancoliver dependenceofmicellesizeandshapeondetergentalkylchainlengthandheadgroup AT janlipfert dependenceofmicellesizeandshapeondetergentalkylchainlengthandheadgroup AT danielafox dependenceofmicellesizeandshapeondetergentalkylchainlengthandheadgroup AT ryanhlo dependenceofmicellesizeandshapeondetergentalkylchainlengthandheadgroup AT sebastiandoniach dependenceofmicellesizeandshapeondetergentalkylchainlengthandheadgroup AT lindacolumbus dependenceofmicellesizeandshapeondetergentalkylchainlengthandheadgroup
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Dependence of micelle size and shape on detergent alkyl chain length and head group.

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