Ultrasound assisted modulation of yeast growth and inactivation kinetics

The yeast Saccharomyces cerevisiae is well known for its application in the food industry for the purpose of developing fermented food. The ultrasound (US) technology offer a wide range of applications for the food industry, including the enhancement of fermentation rates and inactivation of microbi...

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Autores principales:	Arturo B. Soro, Márcia Oliveira, Colm P. O'Donnell, Brijesh K. Tiwari
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Saccharomyces cerevisiae Ultrasound Kinetic models SEM analysis Chemistry QD1-999 Acoustics. Sound QC221-246
Acceso en línea:	https://doaj.org/article/02b3d764c09c4e5184027bb8d18eebda
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spelling	oai:doaj.org-article:02b3d764c09c4e5184027bb8d18eebda2021-12-02T04:59:50ZUltrasound assisted modulation of yeast growth and inactivation kinetics1350-417710.1016/j.ultsonch.2021.105819https://doaj.org/article/02b3d764c09c4e5184027bb8d18eebda2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1350417721003618https://doaj.org/toc/1350-4177The yeast Saccharomyces cerevisiae is well known for its application in the food industry for the purpose of developing fermented food. The ultrasound (US) technology offer a wide range of applications for the food industry, including the enhancement of fermentation rates and inactivation of microbial cells. However, a better understanding and standardization of this technology is still required to ensure the scaling-up process. This study investigated the effect of the US technology on the growth of S. cerevisiae using frequencies of 20, 25, 45 and 130 kHz, treatment periods from 2 to 30 min. Furthermore, yeast kinetics subjected to US treatments were evaluated using modelling tools and scanning electron microscopy (SEM) analysis to explore the impact of sonication on yeast cells. Yeast growth was monitored after different US treatments plotting optical density (OD) at 660 nm for 24 h at 30 ⁰C. Growth curves were fitted using models of modified Gompertz and Scale-Free which showed good parameters of the fit. In particular, US frequencies of 45 and 130 kHz did not have a disruptive effect in lag phase and growth rate of the yeast populations, unlike the frequency of 20 kHz. Moreover, inactivation curves of yeast cells obtained after exposure to 20 and 25 kHz also observed the best fit using the Weibull model. US frequency of 20 kHz achieved significant reductions of 1.3 log cfu/mL in yeast concentration and also induced important cell damage on the external structures of S. cerevisiae. In conclusion, the present study demonstrated the significant effect of applying different US frequencies on the yeast growth for potential application in the food industry.Arturo B. SoroMárcia OliveiraColm P. O'DonnellBrijesh K. TiwariElsevierarticleSaccharomyces cerevisiaeUltrasoundKinetic modelsSEM analysisChemistryQD1-999Acoustics. SoundQC221-246ENUltrasonics Sonochemistry, Vol 80, Iss , Pp 105819- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Saccharomyces cerevisiae Ultrasound Kinetic models SEM analysis Chemistry QD1-999 Acoustics. Sound QC221-246
spellingShingle	Saccharomyces cerevisiae Ultrasound Kinetic models SEM analysis Chemistry QD1-999 Acoustics. Sound QC221-246 Arturo B. Soro Márcia Oliveira Colm P. O'Donnell Brijesh K. Tiwari Ultrasound assisted modulation of yeast growth and inactivation kinetics
description	The yeast Saccharomyces cerevisiae is well known for its application in the food industry for the purpose of developing fermented food. The ultrasound (US) technology offer a wide range of applications for the food industry, including the enhancement of fermentation rates and inactivation of microbial cells. However, a better understanding and standardization of this technology is still required to ensure the scaling-up process. This study investigated the effect of the US technology on the growth of S. cerevisiae using frequencies of 20, 25, 45 and 130 kHz, treatment periods from 2 to 30 min. Furthermore, yeast kinetics subjected to US treatments were evaluated using modelling tools and scanning electron microscopy (SEM) analysis to explore the impact of sonication on yeast cells. Yeast growth was monitored after different US treatments plotting optical density (OD) at 660 nm for 24 h at 30 ⁰C. Growth curves were fitted using models of modified Gompertz and Scale-Free which showed good parameters of the fit. In particular, US frequencies of 45 and 130 kHz did not have a disruptive effect in lag phase and growth rate of the yeast populations, unlike the frequency of 20 kHz. Moreover, inactivation curves of yeast cells obtained after exposure to 20 and 25 kHz also observed the best fit using the Weibull model. US frequency of 20 kHz achieved significant reductions of 1.3 log cfu/mL in yeast concentration and also induced important cell damage on the external structures of S. cerevisiae. In conclusion, the present study demonstrated the significant effect of applying different US frequencies on the yeast growth for potential application in the food industry.
format	article
author	Arturo B. Soro Márcia Oliveira Colm P. O'Donnell Brijesh K. Tiwari
author_facet	Arturo B. Soro Márcia Oliveira Colm P. O'Donnell Brijesh K. Tiwari
author_sort	Arturo B. Soro
title	Ultrasound assisted modulation of yeast growth and inactivation kinetics
title_short	Ultrasound assisted modulation of yeast growth and inactivation kinetics
title_full	Ultrasound assisted modulation of yeast growth and inactivation kinetics
title_fullStr	Ultrasound assisted modulation of yeast growth and inactivation kinetics
title_full_unstemmed	Ultrasound assisted modulation of yeast growth and inactivation kinetics
title_sort	ultrasound assisted modulation of yeast growth and inactivation kinetics
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/02b3d764c09c4e5184027bb8d18eebda
work_keys_str_mv	AT arturobsoro ultrasoundassistedmodulationofyeastgrowthandinactivationkinetics AT marciaoliveira ultrasoundassistedmodulationofyeastgrowthandinactivationkinetics AT colmpodonnell ultrasoundassistedmodulationofyeastgrowthandinactivationkinetics AT brijeshktiwari ultrasoundassistedmodulationofyeastgrowthandinactivationkinetics
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Ultrasound assisted modulation of yeast growth and inactivation kinetics

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