Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide.
Competition and cooperation phenomena occur within highly interactive biofilm communities and several non-biocides molecules produced by microorganisms have been described as impairing biofilm formation. In this study, we investigated the anti-biofilm capacities of an ubiquitous and biofilm producin...
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2014
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oai:doaj.org-article:02bfe77c2bef4ea28b9e8766c8b3f0a02021-11-18T08:15:37ZAnti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide.1932-620310.1371/journal.pone.0099995https://doaj.org/article/02bfe77c2bef4ea28b9e8766c8b3f0a02014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24932475/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Competition and cooperation phenomena occur within highly interactive biofilm communities and several non-biocides molecules produced by microorganisms have been described as impairing biofilm formation. In this study, we investigated the anti-biofilm capacities of an ubiquitous and biofilm producing bacterium, Klebsiella pneumoniae. Cell-free supernatant from K. pneumoniae planktonic cultures showed anti-biofilm effects on most Gram positive bacteria tested but also encompassed some Gram negative bacilli. The anti-biofilm non-bactericidal activity was further investigated on Staphylococcus epidermidis, by determining the biofilm biomass, microscopic observations and agglutination measurement through a magnetic bead-mediated agglutination test. Cell-free extracts from K. pneumoniae biofilm (supernatant and acellular matrix) also showed an influence, although to a lesser extend. Chemical analyses indicated that the active molecule was a high molecular weight polysaccharide composed of five monosaccharides: galactose, glucose, rhamnose, glucuronic acid and glucosamine and the main following sugar linkage residues [→ 2)-α-L-Rhap-(1 →]; [→ 4)-α-L-Rhap-(1 →]; [α-D-Galp-(1 →]; [→ 2,3)-α-D-Galp-(1 →]; [→ 3)-β-D-Galp-(1 →] and, [→ 4)-β-D-GlcAp-(1 →]. Characterization of this molecule indicated that this component was more likely capsular polysaccharide (CPS) and precoating of abiotic surfaces with CPS extracts from different serotypes impaired the bacteria-surface interactions. Thus the CPS of Klebsiella would exhibit a pleiotropic activity during biofilm formation, both stimulating the initial adhesion and maturation steps as previously described, but also repelling potential competitors.Marina Dos Santos GoncalvesCédric DelattreDamien BalestrinoNicolas CharbonnelRedouan ElboutachfaitiAnne WadouachiStéphanie BadelThierry BernardiPhilippe MichaudChristiane ForestierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99995 (2014) |
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Medicine R Science Q Marina Dos Santos Goncalves Cédric Delattre Damien Balestrino Nicolas Charbonnel Redouan Elboutachfaiti Anne Wadouachi Stéphanie Badel Thierry Bernardi Philippe Michaud Christiane Forestier Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide. |
| description |
Competition and cooperation phenomena occur within highly interactive biofilm communities and several non-biocides molecules produced by microorganisms have been described as impairing biofilm formation. In this study, we investigated the anti-biofilm capacities of an ubiquitous and biofilm producing bacterium, Klebsiella pneumoniae. Cell-free supernatant from K. pneumoniae planktonic cultures showed anti-biofilm effects on most Gram positive bacteria tested but also encompassed some Gram negative bacilli. The anti-biofilm non-bactericidal activity was further investigated on Staphylococcus epidermidis, by determining the biofilm biomass, microscopic observations and agglutination measurement through a magnetic bead-mediated agglutination test. Cell-free extracts from K. pneumoniae biofilm (supernatant and acellular matrix) also showed an influence, although to a lesser extend. Chemical analyses indicated that the active molecule was a high molecular weight polysaccharide composed of five monosaccharides: galactose, glucose, rhamnose, glucuronic acid and glucosamine and the main following sugar linkage residues [→ 2)-α-L-Rhap-(1 →]; [→ 4)-α-L-Rhap-(1 →]; [α-D-Galp-(1 →]; [→ 2,3)-α-D-Galp-(1 →]; [→ 3)-β-D-Galp-(1 →] and, [→ 4)-β-D-GlcAp-(1 →]. Characterization of this molecule indicated that this component was more likely capsular polysaccharide (CPS) and precoating of abiotic surfaces with CPS extracts from different serotypes impaired the bacteria-surface interactions. Thus the CPS of Klebsiella would exhibit a pleiotropic activity during biofilm formation, both stimulating the initial adhesion and maturation steps as previously described, but also repelling potential competitors. |
| format |
article |
| author |
Marina Dos Santos Goncalves Cédric Delattre Damien Balestrino Nicolas Charbonnel Redouan Elboutachfaiti Anne Wadouachi Stéphanie Badel Thierry Bernardi Philippe Michaud Christiane Forestier |
| author_facet |
Marina Dos Santos Goncalves Cédric Delattre Damien Balestrino Nicolas Charbonnel Redouan Elboutachfaiti Anne Wadouachi Stéphanie Badel Thierry Bernardi Philippe Michaud Christiane Forestier |
| author_sort |
Marina Dos Santos Goncalves |
| title |
Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide. |
| title_short |
Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide. |
| title_full |
Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide. |
| title_fullStr |
Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide. |
| title_full_unstemmed |
Anti-biofilm activity: a function of Klebsiella pneumoniae capsular polysaccharide. |
| title_sort |
anti-biofilm activity: a function of klebsiella pneumoniae capsular polysaccharide. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/02bfe77c2bef4ea28b9e8766c8b3f0a0 |
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