Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties

Daniela Benedec,1,* Ilioara Oniga,1,* Flavia Cuibus,1 Bogdan Sevastre,2 Gabriela Stiufiuc,3 Mihaela Duma,4 Daniela Hanganu,1 Cristian Iacovita,1 Rares Stiufiuc,1,5 Constantin Mihai Lucaciu1 1Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 2Faculty of...

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Autores principales:	Benedec D, Oniga I, Cuibus F, Sevastre B, Stiufiuc G, Duma M, Hanganu D, Iacovita C, Stiufiuc R, Lucaciu CM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	green nanoparticles synthesis toxicity antioxidant activity Staphyilococcus aureus Candida albicans SERS Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/02c4ef7aaa6947788ad7cbfa1f151ce8
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spelling	oai:doaj.org-article:02c4ef7aaa6947788ad7cbfa1f151ce82021-12-02T07:41:51ZOriganum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties1178-2013https://doaj.org/article/02c4ef7aaa6947788ad7cbfa1f151ce82018-02-01T00:00:00Zhttps://www.dovepress.com/origanum-vulgare-mediated-green-synthesis-of-biocompatible-gold-nanopa-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Daniela Benedec,1,* Ilioara Oniga,1,* Flavia Cuibus,1 Bogdan Sevastre,2 Gabriela Stiufiuc,3 Mihaela Duma,4 Daniela Hanganu,1 Cristian Iacovita,1 Rares Stiufiuc,1,5 Constantin Mihai Lucaciu1 1Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 2Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3Faculty of Physics, “Babeş Bolyai” University, 4State Veterinary Laboratory for Animal Health and Safety, 5Department of Bionanoscopy, MedFuture Research Center for Advance Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania *These authors contributed equally to this work Purpose: The leaves and flowering stem of Origanum vulgare contain essential oils, flavonoids, phenolic acids and anthocyanins. We propose a new, simple, one-pot, O. vulgare extract (OVE) mediated green synthesis method of biocompatible gold nanoparticles (AuNPs) possessing improved antioxidant, antimicrobial and plasmonic properties.Materials and methods: Different concentrations of OVEs were used to reduce gold ions and to synthetize biocompatible spherical AuNPs. Their morphology and physical properties have been investigated by means of transmission electron microscopy, ultraviolet–visible absorption spectroscopy, photon correlation spectroscopy and Fourier transform infrared spectroscopy, whereas their plasmonic properties have been tested using surface-enhanced Raman spectroscopy (SERS). The antioxidant properties of nanoparticles (NPs) have been evaluated by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, and the antimicrobial tests were performed using the disk diffusion assay. Their cytotoxicity has been assessed by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Results: The experimental results confirmed the successful synthesis of biocompatible, spherical, plasmonic NPs having a mean diameter of ~40 nm and an outstanding aqueous stability. This new class of NPs exhibits a very good antioxidant activity and presents interesting inhibitory effects against Staphylococcus aureus and Candida albicans. Due to their plasmonic properties, AuNPs are used as SERS substrates for the detection of a test molecule (methylene blue) up to a concentration of 10-7 M and a pharmaceutical compound (propranolol) in solution. Cytotoxicity assays revealed that AuNPs are better tolerated by normal human dermal fibroblast cells, while the melanoma cancer cells are more sensitive.Conclusion: The biocompatible AuNPs synthetized using OVEs showed significant bactericidal and antimycotic activities, the most sensitive microorganisms being S. aureus and C. albicans, both commonly involved in various dermatological infections. Moreover, the significant antioxidant effect might recommend their use for protective and/or preventive effect in various skin inflammatory conditions, including the reduction in side effects in dermatological infections. Meanwhile, the as-synthesized biocompatible AuNPs can be successfully used as SERS substrates for the detection of pharmaceutical compounds in aqueous solutions. Keywords: green nanoparticles’ synthesis, toxicity, antioxidant activity, Staphylococcus aureus, Candida albicans, SERSBenedec DOniga ICuibus FSevastre BStiufiuc GDuma MHanganu DIacovita CStiufiuc RLucaciu CMDove Medical Pressarticlegreen nanoparticles synthesistoxicityantioxidant activityStaphyilococcus aureusCandida albicansSERSMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1041-1058 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	green nanoparticles synthesis toxicity antioxidant activity Staphyilococcus aureus Candida albicans SERS Medicine (General) R5-920
spellingShingle	green nanoparticles synthesis toxicity antioxidant activity Staphyilococcus aureus Candida albicans SERS Medicine (General) R5-920 Benedec D Oniga I Cuibus F Sevastre B Stiufiuc G Duma M Hanganu D Iacovita C Stiufiuc R Lucaciu CM Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
description	Daniela Benedec,1,* Ilioara Oniga,1,* Flavia Cuibus,1 Bogdan Sevastre,2 Gabriela Stiufiuc,3 Mihaela Duma,4 Daniela Hanganu,1 Cristian Iacovita,1 Rares Stiufiuc,1,5 Constantin Mihai Lucaciu1 1Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 2Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3Faculty of Physics, “Babeş Bolyai” University, 4State Veterinary Laboratory for Animal Health and Safety, 5Department of Bionanoscopy, MedFuture Research Center for Advance Medicine, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania *These authors contributed equally to this work Purpose: The leaves and flowering stem of Origanum vulgare contain essential oils, flavonoids, phenolic acids and anthocyanins. We propose a new, simple, one-pot, O. vulgare extract (OVE) mediated green synthesis method of biocompatible gold nanoparticles (AuNPs) possessing improved antioxidant, antimicrobial and plasmonic properties.Materials and methods: Different concentrations of OVEs were used to reduce gold ions and to synthetize biocompatible spherical AuNPs. Their morphology and physical properties have been investigated by means of transmission electron microscopy, ultraviolet–visible absorption spectroscopy, photon correlation spectroscopy and Fourier transform infrared spectroscopy, whereas their plasmonic properties have been tested using surface-enhanced Raman spectroscopy (SERS). The antioxidant properties of nanoparticles (NPs) have been evaluated by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, and the antimicrobial tests were performed using the disk diffusion assay. Their cytotoxicity has been assessed by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Results: The experimental results confirmed the successful synthesis of biocompatible, spherical, plasmonic NPs having a mean diameter of ~40 nm and an outstanding aqueous stability. This new class of NPs exhibits a very good antioxidant activity and presents interesting inhibitory effects against Staphylococcus aureus and Candida albicans. Due to their plasmonic properties, AuNPs are used as SERS substrates for the detection of a test molecule (methylene blue) up to a concentration of 10-7 M and a pharmaceutical compound (propranolol) in solution. Cytotoxicity assays revealed that AuNPs are better tolerated by normal human dermal fibroblast cells, while the melanoma cancer cells are more sensitive.Conclusion: The biocompatible AuNPs synthetized using OVEs showed significant bactericidal and antimycotic activities, the most sensitive microorganisms being S. aureus and C. albicans, both commonly involved in various dermatological infections. Moreover, the significant antioxidant effect might recommend their use for protective and/or preventive effect in various skin inflammatory conditions, including the reduction in side effects in dermatological infections. Meanwhile, the as-synthesized biocompatible AuNPs can be successfully used as SERS substrates for the detection of pharmaceutical compounds in aqueous solutions. Keywords: green nanoparticles’ synthesis, toxicity, antioxidant activity, Staphylococcus aureus, Candida albicans, SERS
format	article
author	Benedec D Oniga I Cuibus F Sevastre B Stiufiuc G Duma M Hanganu D Iacovita C Stiufiuc R Lucaciu CM
author_facet	Benedec D Oniga I Cuibus F Sevastre B Stiufiuc G Duma M Hanganu D Iacovita C Stiufiuc R Lucaciu CM
author_sort	Benedec D
title	Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
title_short	Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
title_full	Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
title_fullStr	Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
title_full_unstemmed	Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
title_sort	origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/02c4ef7aaa6947788ad7cbfa1f151ce8
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Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties

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