Dichotomy in the NRT gene families of dicots and grass species.

A large proportion of the nitrate (NO(3)(-)) acquired by plants from soil is actively transported via members of the NRT families of NO(3)(-) transporters. In Arabidopsis, the NRT1 family has eight functionally characterised members and predominantly comprises low-affinity transporters; the NRT2 fam...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Darren Plett, John Toubia, Trevor Garnett, Mark Tester, Brent N Kaiser, Ute Baumann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02c5d53a3380457d8fc727dc410bc923
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:02c5d53a3380457d8fc727dc410bc923
record_format	dspace
spelling	oai:doaj.org-article:02c5d53a3380457d8fc727dc410bc9232021-11-18T07:02:02ZDichotomy in the NRT gene families of dicots and grass species.1932-620310.1371/journal.pone.0015289https://doaj.org/article/02c5d53a3380457d8fc727dc410bc9232010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21151904/?tool=EBIhttps://doaj.org/toc/1932-6203A large proportion of the nitrate (NO(3)(-)) acquired by plants from soil is actively transported via members of the NRT families of NO(3)(-) transporters. In Arabidopsis, the NRT1 family has eight functionally characterised members and predominantly comprises low-affinity transporters; the NRT2 family contains seven members which appear to be high-affinity transporters; and there are two NRT3 (NAR2) family members which are known to participate in high-affinity transport. A modified reciprocal best hit (RBH) approach was used to identify putative orthologues of the Arabidopsis NRT genes in the four fully sequenced grass genomes (maize, rice, sorghum, Brachypodium). We also included the poplar genome in our analysis to establish whether differences between Arabidopsis and the grasses may be generally applicable to monocots and dicots. Our analysis reveals fundamental differences between Arabidopsis and the grass species in the gene number and family structure of all three families of NRT transporters. All grass species possessed additional NRT1.1 orthologues and appear to lack NRT1.6/NRT1.7 orthologues. There is significant separation in the NRT2 phylogenetic tree between NRT2 genes from dicots and grass species. This indicates that determination of function of NRT2 genes in grass species will not be possible in cereals based simply on sequence homology to functionally characterised Arabidopsis NRT2 genes and that proper functional analysis will be required. Arabidopsis has a unique NRT3.2 gene which may be a fusion of the NRT3.1 and NRT3.2 genes present in all other species examined here. This work provides a framework for future analysis of NO(3)(-) transporters and NO(3)(-) transport in grass crop species.Darren PlettJohn ToubiaTrevor GarnettMark TesterBrent N KaiserUte BaumannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15289 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Darren Plett John Toubia Trevor Garnett Mark Tester Brent N Kaiser Ute Baumann Dichotomy in the NRT gene families of dicots and grass species.
description	A large proportion of the nitrate (NO(3)(-)) acquired by plants from soil is actively transported via members of the NRT families of NO(3)(-) transporters. In Arabidopsis, the NRT1 family has eight functionally characterised members and predominantly comprises low-affinity transporters; the NRT2 family contains seven members which appear to be high-affinity transporters; and there are two NRT3 (NAR2) family members which are known to participate in high-affinity transport. A modified reciprocal best hit (RBH) approach was used to identify putative orthologues of the Arabidopsis NRT genes in the four fully sequenced grass genomes (maize, rice, sorghum, Brachypodium). We also included the poplar genome in our analysis to establish whether differences between Arabidopsis and the grasses may be generally applicable to monocots and dicots. Our analysis reveals fundamental differences between Arabidopsis and the grass species in the gene number and family structure of all three families of NRT transporters. All grass species possessed additional NRT1.1 orthologues and appear to lack NRT1.6/NRT1.7 orthologues. There is significant separation in the NRT2 phylogenetic tree between NRT2 genes from dicots and grass species. This indicates that determination of function of NRT2 genes in grass species will not be possible in cereals based simply on sequence homology to functionally characterised Arabidopsis NRT2 genes and that proper functional analysis will be required. Arabidopsis has a unique NRT3.2 gene which may be a fusion of the NRT3.1 and NRT3.2 genes present in all other species examined here. This work provides a framework for future analysis of NO(3)(-) transporters and NO(3)(-) transport in grass crop species.
format	article
author	Darren Plett John Toubia Trevor Garnett Mark Tester Brent N Kaiser Ute Baumann
author_facet	Darren Plett John Toubia Trevor Garnett Mark Tester Brent N Kaiser Ute Baumann
author_sort	Darren Plett
title	Dichotomy in the NRT gene families of dicots and grass species.
title_short	Dichotomy in the NRT gene families of dicots and grass species.
title_full	Dichotomy in the NRT gene families of dicots and grass species.
title_fullStr	Dichotomy in the NRT gene families of dicots and grass species.
title_full_unstemmed	Dichotomy in the NRT gene families of dicots and grass species.
title_sort	dichotomy in the nrt gene families of dicots and grass species.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/02c5d53a3380457d8fc727dc410bc923
work_keys_str_mv	AT darrenplett dichotomyinthenrtgenefamiliesofdicotsandgrassspecies AT johntoubia dichotomyinthenrtgenefamiliesofdicotsandgrassspecies AT trevorgarnett dichotomyinthenrtgenefamiliesofdicotsandgrassspecies AT marktester dichotomyinthenrtgenefamiliesofdicotsandgrassspecies AT brentnkaiser dichotomyinthenrtgenefamiliesofdicotsandgrassspecies AT utebaumann dichotomyinthenrtgenefamiliesofdicotsandgrassspecies
_version_	1718424018819743744

Dichotomy in the NRT gene families of dicots and grass species.

Ejemplares similares