Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.

<h4>Aims</h4>Recently, a new defibrillation modality using nanosecond pulses was shown to be effective at much lower energies than conventional 10 millisecond monophasic shocks in ex vivo experiments. Here we compare the safety factors of 300 nanosecond and 10 millisecond shocks to asses...

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Autores principales:	Johanna U Neuber, Andrei G Pakhomov, Christian W Zemlin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02ce03bdcbf84b6fb033460c92a17184
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spelling	oai:doaj.org-article:02ce03bdcbf84b6fb033460c92a171842021-12-02T20:06:10ZElectroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.1932-620310.1371/journal.pone.0257287https://doaj.org/article/02ce03bdcbf84b6fb033460c92a171842021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257287https://doaj.org/toc/1932-6203<h4>Aims</h4>Recently, a new defibrillation modality using nanosecond pulses was shown to be effective at much lower energies than conventional 10 millisecond monophasic shocks in ex vivo experiments. Here we compare the safety factors of 300 nanosecond and 10 millisecond shocks to assess the safety of nanosecond defibrillation.<h4>Methods and results</h4>The safety factor, i.e. the ratio of median effective doses (ED50) for electroporative damage and defibrillation, was assessed for nanosecond and conventional (millisecond) defibrillation shocks in Langendorff-perfused New Zealand white rabbit hearts. In order to allow for multiple shock applications in a single heart, a pair of needle electrodes was used to apply shocks of varying voltage. Propidium iodide (PI) staining at the surface of the heart showed that nanosecond shocks had a slightly lower safety factor (6.50) than millisecond shocks (8.69), p = 0.02; while PI staining cross-sections in the electrode plane showed no significant difference (5.38 for 300 ns shocks and 6.29 for 10 ms shocks, p = 0.22).<h4>Conclusions</h4>In Langendorff-perfused rabbit hearts, nanosecond defibrillation has a similar safety factor as millisecond defibrillation, between 5 and 9, suggesting that nanosecond defibrillation can be performed safely.Johanna U NeuberAndrei G PakhomovChristian W ZemlinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257287 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johanna U Neuber Andrei G Pakhomov Christian W Zemlin Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.
description	<h4>Aims</h4>Recently, a new defibrillation modality using nanosecond pulses was shown to be effective at much lower energies than conventional 10 millisecond monophasic shocks in ex vivo experiments. Here we compare the safety factors of 300 nanosecond and 10 millisecond shocks to assess the safety of nanosecond defibrillation.<h4>Methods and results</h4>The safety factor, i.e. the ratio of median effective doses (ED50) for electroporative damage and defibrillation, was assessed for nanosecond and conventional (millisecond) defibrillation shocks in Langendorff-perfused New Zealand white rabbit hearts. In order to allow for multiple shock applications in a single heart, a pair of needle electrodes was used to apply shocks of varying voltage. Propidium iodide (PI) staining at the surface of the heart showed that nanosecond shocks had a slightly lower safety factor (6.50) than millisecond shocks (8.69), p = 0.02; while PI staining cross-sections in the electrode plane showed no significant difference (5.38 for 300 ns shocks and 6.29 for 10 ms shocks, p = 0.22).<h4>Conclusions</h4>In Langendorff-perfused rabbit hearts, nanosecond defibrillation has a similar safety factor as millisecond defibrillation, between 5 and 9, suggesting that nanosecond defibrillation can be performed safely.
format	article
author	Johanna U Neuber Andrei G Pakhomov Christian W Zemlin
author_facet	Johanna U Neuber Andrei G Pakhomov Christian W Zemlin
author_sort	Johanna U Neuber
title	Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.
title_short	Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.
title_full	Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.
title_fullStr	Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.
title_full_unstemmed	Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.
title_sort	electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in langendorff-perfused rabbit hearts.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/02ce03bdcbf84b6fb033460c92a17184
work_keys_str_mv	AT johannauneuber electroporationsafetyfactorof300nanosecondand10milliseconddefibrillationinlangendorffperfusedrabbithearts AT andreigpakhomov electroporationsafetyfactorof300nanosecondand10milliseconddefibrillationinlangendorffperfusedrabbithearts AT christianwzemlin electroporationsafetyfactorof300nanosecondand10milliseconddefibrillationinlangendorffperfusedrabbithearts
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Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts.

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