Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.

The Sertoli cells are critical regulators of testis differentiation and development. In the adult, however, their known function is restricted largely to maintenance of spermatogenesis. To determine whether the Sertoli cells regulate other aspects of adult testis biology we have used a novel transge...

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Autores principales:	Diane Rebourcet, Peter J O'Shaughnessy, Ana Monteiro, Laura Milne, Lyndsey Cruickshanks, Nathan Jeffrey, Florian Guillou, Tom C Freeman, Rod T Mitchell, Lee B Smith
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02d3787fbc174929a7cbb0de59766329
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spelling	oai:doaj.org-article:02d3787fbc174929a7cbb0de597663292021-11-25T06:03:40ZSertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.1932-620310.1371/journal.pone.0105687https://doaj.org/article/02d3787fbc174929a7cbb0de597663292014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25144714/?tool=EBIhttps://doaj.org/toc/1932-6203The Sertoli cells are critical regulators of testis differentiation and development. In the adult, however, their known function is restricted largely to maintenance of spermatogenesis. To determine whether the Sertoli cells regulate other aspects of adult testis biology we have used a novel transgenic mouse model in which Amh-Cre induces expression of the receptor for Diphtheria toxin (iDTR) specifically within Sertoli cells. This causes controlled, cell-specific and acute ablation of the Sertoli cell population in the adult animal following Diphtheria toxin injection. Results show that Sertoli cell ablation leads to rapid loss of all germ cell populations. In addition, adult Leydig cell numbers decline by 75% with the remaining cells concentrated around the rete and in the sub-capsular region. In the absence of Sertoli cells, peritubular myoid cell activity is reduced but the cells retain an ability to exclude immune cells from the seminiferous tubules. These data demonstrate that, in addition to support of spermatogenesis, Sertoli cells are required in the adult testis both for retention of the normal adult Leydig cell population and for support of normal peritubular myoid cell function. This has implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.Diane RebourcetPeter J O'ShaughnessyAna MonteiroLaura MilneLyndsey CruickshanksNathan JeffreyFlorian GuillouTom C FreemanRod T MitchellLee B SmithPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105687 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diane Rebourcet Peter J O'Shaughnessy Ana Monteiro Laura Milne Lyndsey Cruickshanks Nathan Jeffrey Florian Guillou Tom C Freeman Rod T Mitchell Lee B Smith Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.
description	The Sertoli cells are critical regulators of testis differentiation and development. In the adult, however, their known function is restricted largely to maintenance of spermatogenesis. To determine whether the Sertoli cells regulate other aspects of adult testis biology we have used a novel transgenic mouse model in which Amh-Cre induces expression of the receptor for Diphtheria toxin (iDTR) specifically within Sertoli cells. This causes controlled, cell-specific and acute ablation of the Sertoli cell population in the adult animal following Diphtheria toxin injection. Results show that Sertoli cell ablation leads to rapid loss of all germ cell populations. In addition, adult Leydig cell numbers decline by 75% with the remaining cells concentrated around the rete and in the sub-capsular region. In the absence of Sertoli cells, peritubular myoid cell activity is reduced but the cells retain an ability to exclude immune cells from the seminiferous tubules. These data demonstrate that, in addition to support of spermatogenesis, Sertoli cells are required in the adult testis both for retention of the normal adult Leydig cell population and for support of normal peritubular myoid cell function. This has implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.
format	article
author	Diane Rebourcet Peter J O'Shaughnessy Ana Monteiro Laura Milne Lyndsey Cruickshanks Nathan Jeffrey Florian Guillou Tom C Freeman Rod T Mitchell Lee B Smith
author_facet	Diane Rebourcet Peter J O'Shaughnessy Ana Monteiro Laura Milne Lyndsey Cruickshanks Nathan Jeffrey Florian Guillou Tom C Freeman Rod T Mitchell Lee B Smith
author_sort	Diane Rebourcet
title	Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.
title_short	Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.
title_full	Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.
title_fullStr	Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.
title_full_unstemmed	Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.
title_sort	sertoli cells maintain leydig cell number and peritubular myoid cell activity in the adult mouse testis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/02d3787fbc174929a7cbb0de59766329
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Sertoli cells maintain Leydig cell number and peritubular myoid cell activity in the adult mouse testis.

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