Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes

Abstract The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the ge...

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Autores principales:	Jessica Brandi, Samuele Cheri, Marcello Manfredi, Claudia Di Carlo, Virginia Vita Vanella, Federica Federici, Eleonora Bombiero, Alda Bazaj, Eleonora Rizzi, Laura Manna, Giuseppe Cornaglia, Umberto Marini, Maria Teresa Valenti, Emilio Marengo, Daniela Cecconi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02dd410c31ea4cb6bc51b6104e36a6ab
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spelling	oai:doaj.org-article:02dd410c31ea4cb6bc51b6104e36a6ab2021-12-02T15:32:59ZExploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes10.1038/s41598-020-68483-42045-2322https://doaj.org/article/02dd410c31ea4cb6bc51b6104e36a6ab2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68483-4https://doaj.org/toc/2045-2322Abstract The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogenic effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders.Jessica BrandiSamuele CheriMarcello ManfrediClaudia Di CarloVirginia Vita VanellaFederica FedericiEleonora BombieroAlda BazajEleonora RizziLaura MannaGiuseppe CornagliaUmberto MariniMaria Teresa ValentiEmilio MarengoDaniela CecconiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica Brandi Samuele Cheri Marcello Manfredi Claudia Di Carlo Virginia Vita Vanella Federica Federici Eleonora Bombiero Alda Bazaj Eleonora Rizzi Laura Manna Giuseppe Cornaglia Umberto Marini Maria Teresa Valenti Emilio Marengo Daniela Cecconi Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
description	Abstract The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogenic effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders.
format	article
author	Jessica Brandi Samuele Cheri Marcello Manfredi Claudia Di Carlo Virginia Vita Vanella Federica Federici Eleonora Bombiero Alda Bazaj Eleonora Rizzi Laura Manna Giuseppe Cornaglia Umberto Marini Maria Teresa Valenti Emilio Marengo Daniela Cecconi
author_facet	Jessica Brandi Samuele Cheri Marcello Manfredi Claudia Di Carlo Virginia Vita Vanella Federica Federici Eleonora Bombiero Alda Bazaj Eleonora Rizzi Laura Manna Giuseppe Cornaglia Umberto Marini Maria Teresa Valenti Emilio Marengo Daniela Cecconi
author_sort	Jessica Brandi
title	Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
title_short	Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
title_full	Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
title_fullStr	Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
title_full_unstemmed	Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
title_sort	exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/02dd410c31ea4cb6bc51b6104e36a6ab
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Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes

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