Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kina...
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | article |
| Langue: | EN |
| Publié: |
IWA Publishing
2021
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| Sujets: | |
| Accès en ligne: | https://doaj.org/article/02decb8972e2485a98c2bb022f308ad5 |
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| Résumé: | The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kinase (STK) genes expression of cyanobacterium Microcystis aeruginosa in response to light limitation. The results showed M. aeruginosa exhibited overcompensatory growth for 2 days after light recovery, during which the increase in growth was inversely related to light intensity. Expression of STK genes, such as spkD, was upregulated significantly at 0.5–4 h after light recovery (P < 0.05). To investigate the function of STK genes in the overcompensatory growth, M. aeruginosa spkD was heterologously expressed in Synechocystis. Transgenic Synechocystis exhibited greater and longer overcompensatory growth than wild-type Synechocystis after light recovery. Relative expression levels of STK genes in transgenic Synechocystis were significantly higher than those in wild-type Synechocystis at 24 h of light recovery (P < 0.05). Heterologous expression of Microcystis spkD might stimulate overcompensatory growth of Synechocystis by affecting its STK gene expression. HIGHLIGHTS
Light limitation induced overcompensatory growth of Microcystis.;
Light limitation affected expression of most STK genes of Microcystis.;
Heterologous expression of Microcystis spkD enhanced and prolonged light-limitation-induced overcompensatory growth of transgenic Synechocystis.;
Heterologous expression of Microcystis spkD stimulated relative expression levels of STK genes in transgenic Synechocystis.; |
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