Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved

The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kina...

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Autores principales:	Wei Dai, Gao Chen, Xiangdong Bi, Huairong Zhong, Xueying Wang, Shaojie Dong, Dong Lv, Shulin Zhang, Dajuan Zhang, Na Wang
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	compensatory growth cyanobacteria light limitation serine/threonine kinase spkd Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/02decb8972e2485a98c2bb022f308ad5
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spelling	oai:doaj.org-article:02decb8972e2485a98c2bb022f308ad52021-11-06T10:52:28ZLight limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved0273-12231996-973210.2166/wst.2021.071https://doaj.org/article/02decb8972e2485a98c2bb022f308ad52021-03-01T00:00:00Zhttp://wst.iwaponline.com/content/83/6/1347https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kinase (STK) genes expression of cyanobacterium Microcystis aeruginosa in response to light limitation. The results showed M. aeruginosa exhibited overcompensatory growth for 2 days after light recovery, during which the increase in growth was inversely related to light intensity. Expression of STK genes, such as spkD, was upregulated significantly at 0.5–4 h after light recovery (P < 0.05). To investigate the function of STK genes in the overcompensatory growth, M. aeruginosa spkD was heterologously expressed in Synechocystis. Transgenic Synechocystis exhibited greater and longer overcompensatory growth than wild-type Synechocystis after light recovery. Relative expression levels of STK genes in transgenic Synechocystis were significantly higher than those in wild-type Synechocystis at 24 h of light recovery (P < 0.05). Heterologous expression of Microcystis spkD might stimulate overcompensatory growth of Synechocystis by affecting its STK gene expression. HIGHLIGHTS Light limitation induced overcompensatory growth of Microcystis.; Light limitation affected expression of most STK genes of Microcystis.; Heterologous expression of Microcystis spkD enhanced and prolonged light-limitation-induced overcompensatory growth of transgenic Synechocystis.; Heterologous expression of Microcystis spkD stimulated relative expression levels of STK genes in transgenic Synechocystis.;Wei DaiGao ChenXiangdong BiHuairong ZhongXueying WangShaojie DongDong LvShulin ZhangDajuan ZhangNa WangIWA Publishingarticlecompensatory growthcyanobacterialight limitationserine/threonine kinasespkdEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 6, Pp 1347-1356 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	compensatory growth cyanobacteria light limitation serine/threonine kinase spkd Environmental technology. Sanitary engineering TD1-1066
spellingShingle	compensatory growth cyanobacteria light limitation serine/threonine kinase spkd Environmental technology. Sanitary engineering TD1-1066 Wei Dai Gao Chen Xiangdong Bi Huairong Zhong Xueying Wang Shaojie Dong Dong Lv Shulin Zhang Dajuan Zhang Na Wang Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
description	The rapid overcompensatory growth that appears when cyanobacteria are supplied with adequate resources after a period of resource deprivation might contribute to the occurrence of cyanobacterial blooms. We investigated the changing characteristics of overcompensatory growth and serine/threonine kinase (STK) genes expression of cyanobacterium Microcystis aeruginosa in response to light limitation. The results showed M. aeruginosa exhibited overcompensatory growth for 2 days after light recovery, during which the increase in growth was inversely related to light intensity. Expression of STK genes, such as spkD, was upregulated significantly at 0.5–4 h after light recovery (P < 0.05). To investigate the function of STK genes in the overcompensatory growth, M. aeruginosa spkD was heterologously expressed in Synechocystis. Transgenic Synechocystis exhibited greater and longer overcompensatory growth than wild-type Synechocystis after light recovery. Relative expression levels of STK genes in transgenic Synechocystis were significantly higher than those in wild-type Synechocystis at 24 h of light recovery (P < 0.05). Heterologous expression of Microcystis spkD might stimulate overcompensatory growth of Synechocystis by affecting its STK gene expression. HIGHLIGHTS Light limitation induced overcompensatory growth of Microcystis.; Light limitation affected expression of most STK genes of Microcystis.; Heterologous expression of Microcystis spkD enhanced and prolonged light-limitation-induced overcompensatory growth of transgenic Synechocystis.; Heterologous expression of Microcystis spkD stimulated relative expression levels of STK genes in transgenic Synechocystis.;
format	article
author	Wei Dai Gao Chen Xiangdong Bi Huairong Zhong Xueying Wang Shaojie Dong Dong Lv Shulin Zhang Dajuan Zhang Na Wang
author_facet	Wei Dai Gao Chen Xiangdong Bi Huairong Zhong Xueying Wang Shaojie Dong Dong Lv Shulin Zhang Dajuan Zhang Na Wang
author_sort	Wei Dai
title	Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
title_short	Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
title_full	Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
title_fullStr	Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
title_full_unstemmed	Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved
title_sort	light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (stk) genes involved
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/02decb8972e2485a98c2bb022f308ad5
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Light limitation inducing overcompensatory growth of cyanobacteria and function of serine/threonine kinase (STK) genes involved

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