Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)

Sunflower occupies the fourth position among oilseed crops the around the world. Eceriferum (CER) is an important gene family that plays critical role in very-long-chain fatty acids elongation and biosynthesis of epicuticular waxes under both biotic and abiotic stress conditions. The aim of present...

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Autores principales:	Hafiz Muhammad Ahmad, Xiukang Wang, Sajid Fiaz, Mahmood-Ur-Rahman, Muhammad Azhar Nadeem, Sher Aslam Khan, Sunny Ahmar, Farrukh Azeem, Tayyaba Shaheen, Freddy Mora-Poblete
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Sunflower CER genes Genome wide analysis Drought stress Wax biosynthesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/02e0981acc4e4f3c90d6d3c78421f3a5
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spelling	oai:doaj.org-article:02e0981acc4e4f3c90d6d3c78421f3a52021-11-20T04:56:35ZComprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)1319-562X10.1016/j.sjbs.2021.07.077https://doaj.org/article/02e0981acc4e4f3c90d6d3c78421f3a52021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006604https://doaj.org/toc/1319-562XSunflower occupies the fourth position among oilseed crops the around the world. Eceriferum (CER) is an important gene family that plays critical role in very-long-chain fatty acids elongation and biosynthesis of epicuticular waxes under both biotic and abiotic stress conditions. The aim of present study was to investigate the effect of sunflower CER genes during drought stress condition. Thus, comparative analysis was undertaken for sunflower CER genes with Arabidopsis genome to determine phylogenetic relationship, chromosomal mapping, gene structures, gene ontology and conserved motifs. Furthermore, we subjected the sunflower cultivars under drought stress and used qRT-PCR analysis to explore the expression pattern of CER genes during drought conditions. We identified thirty-seven unevenly distributed CER genes in the sunflower genome. The phylogenetic analysis revealed that CER genes were grouped into seven clades in Arabidopsis, Helianthus annuus, and Gossypium hirsutum. Expression analysis showed that genes CER10 and CER60 were upregulated in sunflower during drought conditions, indicating that these genes are activated during drought stress. The results obtained will serve to characterize the CER gene family in sunflower and exploit the role of these genes in wax biosynthesis under limited water conditions. Key message: Cuticular waxes protect the plants from drought stress, so we observed the expression of wax bio synthesis genes in recently sequences genome of Helianthus annuus. We observed that expression of wax biosynthesis genes CER10 and CER60 was upregulated when the plants were subjected to drought stress.Hafiz Muhammad AhmadXiukang WangSajid Fiaz Mahmood-Ur-RahmanMuhammad Azhar NadeemSher Aslam KhanSunny AhmarFarrukh AzeemTayyaba ShaheenFreddy Mora-PobleteElsevierarticleSunflowerCER genesGenome wide analysisDrought stressWax biosynthesisBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6884-6896 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sunflower CER genes Genome wide analysis Drought stress Wax biosynthesis Biology (General) QH301-705.5
spellingShingle	Sunflower CER genes Genome wide analysis Drought stress Wax biosynthesis Biology (General) QH301-705.5 Hafiz Muhammad Ahmad Xiukang Wang Sajid Fiaz Mahmood-Ur-Rahman Muhammad Azhar Nadeem Sher Aslam Khan Sunny Ahmar Farrukh Azeem Tayyaba Shaheen Freddy Mora-Poblete Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)
description	Sunflower occupies the fourth position among oilseed crops the around the world. Eceriferum (CER) is an important gene family that plays critical role in very-long-chain fatty acids elongation and biosynthesis of epicuticular waxes under both biotic and abiotic stress conditions. The aim of present study was to investigate the effect of sunflower CER genes during drought stress condition. Thus, comparative analysis was undertaken for sunflower CER genes with Arabidopsis genome to determine phylogenetic relationship, chromosomal mapping, gene structures, gene ontology and conserved motifs. Furthermore, we subjected the sunflower cultivars under drought stress and used qRT-PCR analysis to explore the expression pattern of CER genes during drought conditions. We identified thirty-seven unevenly distributed CER genes in the sunflower genome. The phylogenetic analysis revealed that CER genes were grouped into seven clades in Arabidopsis, Helianthus annuus, and Gossypium hirsutum. Expression analysis showed that genes CER10 and CER60 were upregulated in sunflower during drought conditions, indicating that these genes are activated during drought stress. The results obtained will serve to characterize the CER gene family in sunflower and exploit the role of these genes in wax biosynthesis under limited water conditions. Key message: Cuticular waxes protect the plants from drought stress, so we observed the expression of wax bio synthesis genes in recently sequences genome of Helianthus annuus. We observed that expression of wax biosynthesis genes CER10 and CER60 was upregulated when the plants were subjected to drought stress.
format	article
author	Hafiz Muhammad Ahmad Xiukang Wang Sajid Fiaz Mahmood-Ur-Rahman Muhammad Azhar Nadeem Sher Aslam Khan Sunny Ahmar Farrukh Azeem Tayyaba Shaheen Freddy Mora-Poblete
author_facet	Hafiz Muhammad Ahmad Xiukang Wang Sajid Fiaz Mahmood-Ur-Rahman Muhammad Azhar Nadeem Sher Aslam Khan Sunny Ahmar Farrukh Azeem Tayyaba Shaheen Freddy Mora-Poblete
author_sort	Hafiz Muhammad Ahmad
title	Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)
title_short	Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)
title_full	Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)
title_fullStr	Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)
title_full_unstemmed	Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)
title_sort	comprehensive genomics and expression analysis of eceriferum (cer) genes in sunflower (helianthus annuus)
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/02e0981acc4e4f3c90d6d3c78421f3a5
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Comprehensive genomics and expression analysis of eceriferum (CER) genes in sunflower (Helianthus annuus)

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