High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake

Abstract Small extracellular vesicles (sEVs) play a key role in intercellular communication. Cargo molecules carried by sEVs may affect the phenotype and function of recipient cells. Epithelial cancer cell‐derived sEVs, particularly those enriched in CD151 or tetraspanin8 (TSPAN8) and associated int...

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Autores principales:	Teng Wang, Xin Wang, Haobin Wang, Luhan Li, Chenhong Zhang, Rong Xiang, Xiaoyue Tan, Zongjin Li, Chunyang Jiang, Lei Zheng, Lehui Xiao, Shijing Yue
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	confined diffusion metastasis single particle tracking small extracellular vesicles TSPAN8 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/02e2f413342a4456b7005a365ed87ab3
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spelling	oai:doaj.org-article:02e2f413342a4456b7005a365ed87ab32021-11-24T14:04:30ZHigh TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake2001-307810.1002/jev2.12167https://doaj.org/article/02e2f413342a4456b7005a365ed87ab32021-11-01T00:00:00Zhttps://doi.org/10.1002/jev2.12167https://doaj.org/toc/2001-3078Abstract Small extracellular vesicles (sEVs) play a key role in intercellular communication. Cargo molecules carried by sEVs may affect the phenotype and function of recipient cells. Epithelial cancer cell‐derived sEVs, particularly those enriched in CD151 or tetraspanin8 (TSPAN8) and associated integrins, promote tumour progression. The mechanism of binding and modulation of sEVs to recipient cells remains elusive. Here, we used genetically engineered breast cancer cells to derive TSPAN8‐enriched sEVs and evaluated the impact of TSPAN8 on target cell membrane's diffusion and transport properties. The single‐particle tracking technique showed that TSPAN8 significantly promoted sEV binding via confined diffusion. Functional assays indicated that the transgenic TSPAN8‐sEV cargo increased cancer cell motility and epithelial‐mesenchymal transition (EMT). In vivo, transgenic TSPAN8‐sEV promoted uptake of sEVs in the liver, lung, and spleen. We concluded that TSPAN8 encourages the sEV‐target cell interaction via forced confined diffusion and significantly increases cell motility. Therefore, TSPAN8‐sEV may serve as an important direct or indirect therapeutic target.Teng WangXin WangHaobin WangLuhan LiChenhong ZhangRong XiangXiaoyue TanZongjin LiChunyang JiangLei ZhengLehui XiaoShijing YueTaylor & Francis Grouparticleconfined diffusionmetastasissingle particle trackingsmall extracellular vesiclesTSPAN8CytologyQH573-671ENJournal of Extracellular Vesicles, Vol 10, Iss 13, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	confined diffusion metastasis single particle tracking small extracellular vesicles TSPAN8 Cytology QH573-671
spellingShingle	confined diffusion metastasis single particle tracking small extracellular vesicles TSPAN8 Cytology QH573-671 Teng Wang Xin Wang Haobin Wang Luhan Li Chenhong Zhang Rong Xiang Xiaoyue Tan Zongjin Li Chunyang Jiang Lei Zheng Lehui Xiao Shijing Yue High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
description	Abstract Small extracellular vesicles (sEVs) play a key role in intercellular communication. Cargo molecules carried by sEVs may affect the phenotype and function of recipient cells. Epithelial cancer cell‐derived sEVs, particularly those enriched in CD151 or tetraspanin8 (TSPAN8) and associated integrins, promote tumour progression. The mechanism of binding and modulation of sEVs to recipient cells remains elusive. Here, we used genetically engineered breast cancer cells to derive TSPAN8‐enriched sEVs and evaluated the impact of TSPAN8 on target cell membrane's diffusion and transport properties. The single‐particle tracking technique showed that TSPAN8 significantly promoted sEV binding via confined diffusion. Functional assays indicated that the transgenic TSPAN8‐sEV cargo increased cancer cell motility and epithelial‐mesenchymal transition (EMT). In vivo, transgenic TSPAN8‐sEV promoted uptake of sEVs in the liver, lung, and spleen. We concluded that TSPAN8 encourages the sEV‐target cell interaction via forced confined diffusion and significantly increases cell motility. Therefore, TSPAN8‐sEV may serve as an important direct or indirect therapeutic target.
format	article
author	Teng Wang Xin Wang Haobin Wang Luhan Li Chenhong Zhang Rong Xiang Xiaoyue Tan Zongjin Li Chunyang Jiang Lei Zheng Lehui Xiao Shijing Yue
author_facet	Teng Wang Xin Wang Haobin Wang Luhan Li Chenhong Zhang Rong Xiang Xiaoyue Tan Zongjin Li Chunyang Jiang Lei Zheng Lehui Xiao Shijing Yue
author_sort	Teng Wang
title	High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
title_short	High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
title_full	High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
title_fullStr	High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
title_full_unstemmed	High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
title_sort	high tspan8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/02e2f413342a4456b7005a365ed87ab3
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_version_	1718415027423150080

High TSPAN8 expression in epithelial cancer cell‐derived small extracellular vesicles promote confined diffusion and pronounced uptake

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