Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.

Creb (Cyclic AMP response element binding protein) is a nuclear regulatory factor that regulates transcription through autophosphorylation. In melanocytes, cAMP's corresponding elements bind to the Creb protein to autophosphorylation and activate MITF (Microphthalmia-associated transcription fa...

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Autores principales:	Mengying Zhang, Xiajun Chen, Jinpan Zhang, Jiale Li, Zhiyi Bai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/02f471fbfb534e23bcdbd7c35f0633f7
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spelling	oai:doaj.org-article:02f471fbfb534e23bcdbd7c35f0633f72021-11-25T06:23:45ZCloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.1932-620310.1371/journal.pone.0251452https://doaj.org/article/02f471fbfb534e23bcdbd7c35f0633f72021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251452https://doaj.org/toc/1932-6203Creb (Cyclic AMP response element binding protein) is a nuclear regulatory factor that regulates transcription through autophosphorylation. In melanocytes, cAMP's corresponding elements bind to the Creb protein to autophosphorylation and activate MITF (Microphthalmia-associated transcription factor). MITF stimulates Tyrosine(tyr) to induce melanocytes to differentiate into eumelanin and pheomelanin. In this study, a HcCreb gene in Hyriopsis cumingii was cloned and its effects on melanin synthesis and nacre color were studied. HcCreb was expressed in both purple and white mussels, and there was a significant difference in expression between adductor muscle (p<0.01) and mantle tissue (p<0.05). Other tissues did not show significant differences (except for gill tissue), and in general, the level of mRNA expression was higher in purple mussels than in white mussels. In both white and purple mussels expression levels in gill tissue was the highest, followed by the mantle. Strong and specific mRNA signals were detected in the dorsal epithelial cells of the mantle pallial layer, indicating that HcCreb may be involved in nacre formation. After arbutin treatment, the expression of HcCreb decreased significantly. By further testing the changes in mantle melanin content it was found that the melanin content after arbutin treatment decreased significantly compared to the control group (p<0.05). It is speculated that the HcCreb gene plays a role in the process of melanin synthesis and nacre color formation in H. cumingii.Mengying ZhangXiajun ChenJinpan ZhangJiale LiZhiyi BaiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251452 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mengying Zhang Xiajun Chen Jinpan Zhang Jiale Li Zhiyi Bai Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.
description	Creb (Cyclic AMP response element binding protein) is a nuclear regulatory factor that regulates transcription through autophosphorylation. In melanocytes, cAMP's corresponding elements bind to the Creb protein to autophosphorylation and activate MITF (Microphthalmia-associated transcription factor). MITF stimulates Tyrosine(tyr) to induce melanocytes to differentiate into eumelanin and pheomelanin. In this study, a HcCreb gene in Hyriopsis cumingii was cloned and its effects on melanin synthesis and nacre color were studied. HcCreb was expressed in both purple and white mussels, and there was a significant difference in expression between adductor muscle (p<0.01) and mantle tissue (p<0.05). Other tissues did not show significant differences (except for gill tissue), and in general, the level of mRNA expression was higher in purple mussels than in white mussels. In both white and purple mussels expression levels in gill tissue was the highest, followed by the mantle. Strong and specific mRNA signals were detected in the dorsal epithelial cells of the mantle pallial layer, indicating that HcCreb may be involved in nacre formation. After arbutin treatment, the expression of HcCreb decreased significantly. By further testing the changes in mantle melanin content it was found that the melanin content after arbutin treatment decreased significantly compared to the control group (p<0.05). It is speculated that the HcCreb gene plays a role in the process of melanin synthesis and nacre color formation in H. cumingii.
format	article
author	Mengying Zhang Xiajun Chen Jinpan Zhang Jiale Li Zhiyi Bai
author_facet	Mengying Zhang Xiajun Chen Jinpan Zhang Jiale Li Zhiyi Bai
author_sort	Mengying Zhang
title	Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.
title_short	Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.
title_full	Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.
title_fullStr	Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.
title_full_unstemmed	Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.
title_sort	cloning of a hccreb gene and analysis of its effects on nacre color and melanin synthesis in hyriopsis cumingii.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/02f471fbfb534e23bcdbd7c35f0633f7
work_keys_str_mv	AT mengyingzhang cloningofahccrebgeneandanalysisofitseffectsonnacrecolorandmelaninsynthesisinhyriopsiscumingii AT xiajunchen cloningofahccrebgeneandanalysisofitseffectsonnacrecolorandmelaninsynthesisinhyriopsiscumingii AT jinpanzhang cloningofahccrebgeneandanalysisofitseffectsonnacrecolorandmelaninsynthesisinhyriopsiscumingii AT jialeli cloningofahccrebgeneandanalysisofitseffectsonnacrecolorandmelaninsynthesisinhyriopsiscumingii AT zhiyibai cloningofahccrebgeneandanalysisofitseffectsonnacrecolorandmelaninsynthesisinhyriopsiscumingii
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Cloning of a HcCreb gene and analysis of its effects on nacre color and melanin synthesis in Hyriopsis cumingii.

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