Argonaute bypasses cellular obstacles without hindrance during target search

Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and slides along the DNA during target search.

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Autores principales:	Tao Ju Cui, Misha Klein, Jorrit W. Hegge, Stanley D. Chandradoss, John van der Oost, Martin Depken, Chirlmin Joo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/030bdb4c6a084d029bfa423bfee6d58d
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spelling	oai:doaj.org-article:030bdb4c6a084d029bfa423bfee6d58d2021-12-02T16:58:01ZArgonaute bypasses cellular obstacles without hindrance during target search10.1038/s41467-019-12415-y2041-1723https://doaj.org/article/030bdb4c6a084d029bfa423bfee6d58d2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12415-yhttps://doaj.org/toc/2041-1723Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and slides along the DNA during target search.Tao Ju CuiMisha KleinJorrit W. HeggeStanley D. ChandradossJohn van der OostMartin DepkenChirlmin JooNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tao Ju Cui Misha Klein Jorrit W. Hegge Stanley D. Chandradoss John van der Oost Martin Depken Chirlmin Joo Argonaute bypasses cellular obstacles without hindrance during target search
description	Argonaute protein is loaded with small RNA and scans long stretch of sequences to find complementary target sites. Here, using single-molecule FRET and kinetic modelling, the authors showed that prokaryotic Argonaute protein binds target DNA loosely and slides along the DNA during target search.
format	article
author	Tao Ju Cui Misha Klein Jorrit W. Hegge Stanley D. Chandradoss John van der Oost Martin Depken Chirlmin Joo
author_facet	Tao Ju Cui Misha Klein Jorrit W. Hegge Stanley D. Chandradoss John van der Oost Martin Depken Chirlmin Joo
author_sort	Tao Ju Cui
title	Argonaute bypasses cellular obstacles without hindrance during target search
title_short	Argonaute bypasses cellular obstacles without hindrance during target search
title_full	Argonaute bypasses cellular obstacles without hindrance during target search
title_fullStr	Argonaute bypasses cellular obstacles without hindrance during target search
title_full_unstemmed	Argonaute bypasses cellular obstacles without hindrance during target search
title_sort	argonaute bypasses cellular obstacles without hindrance during target search
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/030bdb4c6a084d029bfa423bfee6d58d
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Argonaute bypasses cellular obstacles without hindrance during target search

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