<i>Arabidopsis</i> SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering
The changes in histone acetylation mediated by histone deacetylases (HDAC) play a crucial role in plant development and response to environmental changes. Mammalian HDACs are regulated by post-translational modifications (PTM), such as phosphorylation, acetylation, ubiquitination and small ubiquitin...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/031578a34acb4d499658902994e36e03 |
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| Sumario: | The changes in histone acetylation mediated by histone deacetylases (HDAC) play a crucial role in plant development and response to environmental changes. Mammalian HDACs are regulated by post-translational modifications (PTM), such as phosphorylation, acetylation, ubiquitination and small ubiquitin-like modifier (SUMO) modification (SUMOylation), which affect enzymatic activity and transcriptional repression. Whether PTMs of plant HDACs alter their functions are largely unknown. In this study, we demonstrated that the <i>Arabidopsis</i> SUMO E3 ligase SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1) interacts with HISTONE DEACETYLASE 6 (HDA6) both in vitro and in vivo. Biochemical analyses indicated that HDA6 is not modified by SUMO1. Overexpression of <i>HDA6</i> in <i>siz1-3</i> background results in a decreased level of histone H3 acetylation, indicating that the activity of HDA6 is increased in <i>siz1-3</i> plants. Chromatin immunoprecipitation (ChIP) assays showed that SIZ1 represses HDA6 binding to its target genes <i>FLOWERING LOCUS C</i> (<i>FLC</i>) and <i>MADS AFFECTING FLOWERING 4</i> (<i>MAF4</i>), resulting in the upregulation of <i>FLC</i> and <i>MAF4</i> by increasing the level of histone H3 acetylation. Together, these findings indicate that the <i>Arabidopsis</i> SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates HDA6 function. |
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